New strategy to improve quality control of Montenegro skin test at the production level
Abstract INTRODUCTION: The production of the Montenegro antigen for skin test poses difficulties regarding quality control. Here, we propose that certain animal models reproducing a similar immune response to humans may be used in the quality control of Montenegro antigen production. METHODS: Fiftee...
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ftdoajarticles:oai:doaj.org/article:f5e5dbfc7195415590d2a3ab8f59315b 2023-05-15T15:06:36+02:00 New strategy to improve quality control of Montenegro skin test at the production level Deborah Carbonera Guedes João Carlos Minozzo Aline Kuhn Sbruzzi Pasquali Craig Faulds Carlos Ricardo Soccol Vanete Thomaz-Soccol https://doi.org/10.1590/0037-8682-0131-2017 https://doaj.org/article/f5e5dbfc7195415590d2a3ab8f59315b EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822017000600788&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0131-2017 https://doaj.org/article/f5e5dbfc7195415590d2a3ab8f59315b Revista da Sociedade Brasileira de Medicina Tropical, Vol 50, Iss 6, Pp 788-794 Cutaneous leishmaniasis diagnosis Montenegro skin test Cavia porcellus Arctic medicine. Tropical medicine RC955-962 article ftdoajarticles https://doi.org/10.1590/0037-8682-0131-2017 2022-12-30T23:07:13Z Abstract INTRODUCTION: The production of the Montenegro antigen for skin test poses difficulties regarding quality control. Here, we propose that certain animal models reproducing a similar immune response to humans may be used in the quality control of Montenegro antigen production. METHODS: Fifteen Cavia porcellus (guinea pigs) were immunized with Leishmania amazonensis or Leishmania braziliensis , and, after 30 days, they were skin tested with standard Montenegro antigen. To validate C. porcellus as an animal model for skin tests, eighteen Mesocricetus auratus (hamsters) were infected with L. amazonensis or L. braziliensis , and, after 45 days, they were skin tested with standard Montenegro antigen. RESULTS: Cavia porcellus immunized with L. amazonensis or L. braziliensis , and hamsters infected with the same species presented induration reactions when skin tested with standard Montenegro antigen 48-72h after the test. CONCLUSIONS: The comparison between immunization methods and immune response from the two animal species validated C. porcellus as a good model for Montenegro skin test, and the model showed strong potential as an in vivo model in the quality control of the production of Montenegro antigen. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 50 6 788 794 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
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Cutaneous leishmaniasis diagnosis Montenegro skin test Cavia porcellus Arctic medicine. Tropical medicine RC955-962 |
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Cutaneous leishmaniasis diagnosis Montenegro skin test Cavia porcellus Arctic medicine. Tropical medicine RC955-962 Deborah Carbonera Guedes João Carlos Minozzo Aline Kuhn Sbruzzi Pasquali Craig Faulds Carlos Ricardo Soccol Vanete Thomaz-Soccol New strategy to improve quality control of Montenegro skin test at the production level |
topic_facet |
Cutaneous leishmaniasis diagnosis Montenegro skin test Cavia porcellus Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract INTRODUCTION: The production of the Montenegro antigen for skin test poses difficulties regarding quality control. Here, we propose that certain animal models reproducing a similar immune response to humans may be used in the quality control of Montenegro antigen production. METHODS: Fifteen Cavia porcellus (guinea pigs) were immunized with Leishmania amazonensis or Leishmania braziliensis , and, after 30 days, they were skin tested with standard Montenegro antigen. To validate C. porcellus as an animal model for skin tests, eighteen Mesocricetus auratus (hamsters) were infected with L. amazonensis or L. braziliensis , and, after 45 days, they were skin tested with standard Montenegro antigen. RESULTS: Cavia porcellus immunized with L. amazonensis or L. braziliensis , and hamsters infected with the same species presented induration reactions when skin tested with standard Montenegro antigen 48-72h after the test. CONCLUSIONS: The comparison between immunization methods and immune response from the two animal species validated C. porcellus as a good model for Montenegro skin test, and the model showed strong potential as an in vivo model in the quality control of the production of Montenegro antigen. |
format |
Article in Journal/Newspaper |
author |
Deborah Carbonera Guedes João Carlos Minozzo Aline Kuhn Sbruzzi Pasquali Craig Faulds Carlos Ricardo Soccol Vanete Thomaz-Soccol |
author_facet |
Deborah Carbonera Guedes João Carlos Minozzo Aline Kuhn Sbruzzi Pasquali Craig Faulds Carlos Ricardo Soccol Vanete Thomaz-Soccol |
author_sort |
Deborah Carbonera Guedes |
title |
New strategy to improve quality control of Montenegro skin test at the production level |
title_short |
New strategy to improve quality control of Montenegro skin test at the production level |
title_full |
New strategy to improve quality control of Montenegro skin test at the production level |
title_fullStr |
New strategy to improve quality control of Montenegro skin test at the production level |
title_full_unstemmed |
New strategy to improve quality control of Montenegro skin test at the production level |
title_sort |
new strategy to improve quality control of montenegro skin test at the production level |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
url |
https://doi.org/10.1590/0037-8682-0131-2017 https://doaj.org/article/f5e5dbfc7195415590d2a3ab8f59315b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 50, Iss 6, Pp 788-794 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822017000600788&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0131-2017 https://doaj.org/article/f5e5dbfc7195415590d2a3ab8f59315b |
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https://doi.org/10.1590/0037-8682-0131-2017 |
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