Detection of Trypanosoma spp. in Bandicota indica from the Thai-Myanmar border area, Mae Sot District Tak Province, Thailand
Objective: To investigate the prevalence of trypanosome infection and their phylogeny in Bandicota indica rats from the cadmium-contaminated area of Mae Sot and the Myanmar border. Methods: Blood samples were taken from 100 animals, and parasite infection was examined by light microscopy observation...
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Wolters Kluwer Medknow Publications
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Online Access: | https://doi.org/10.4103/1995-7645.269907 https://doaj.org/article/f5c4e2e68e6e4dc3a7cc66aa33055540 |
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ftdoajarticles:oai:doaj.org/article:f5c4e2e68e6e4dc3a7cc66aa33055540 2023-05-15T15:05:18+02:00 Detection of Trypanosoma spp. in Bandicota indica from the Thai-Myanmar border area, Mae Sot District Tak Province, Thailand Phuangphet Waree Molee Natthiya Sakulsak Somchai Saengamnatdej 2019-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.269907 https://doaj.org/article/f5c4e2e68e6e4dc3a7cc66aa33055540 EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=10;spage=457;epage=462;aulast=Molee https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.269907 https://doaj.org/article/f5c4e2e68e6e4dc3a7cc66aa33055540 Asian Pacific Journal of Tropical Medicine, Vol 12, Iss 10, Pp 457-462 (2019) trypanosoma spp polymerase chain reaction phylogenetic analysis zoonoses bandicota indica Arctic medicine. Tropical medicine RC955-962 article 2019 ftdoajarticles https://doi.org/10.4103/1995-7645.269907 2022-12-31T09:28:44Z Objective: To investigate the prevalence of trypanosome infection and their phylogeny in Bandicota indica rats from the cadmium-contaminated area of Mae Sot and the Myanmar border. Methods: Blood samples were taken from 100 animals, and parasite infection was examined by light microscopy observation and polymerase chain reaction (PCR) studies. Results: Trypanosoma spp. infection was found in 20% of the thin blood smear samples. PCR showed positive 623 bp DNA bands in 21 samples (21%). The sequencing analysis showed that all of the samples (100%) had the Trypanasoma lewisi 18S ribosomal RNA gene. Phylogenetic analysis confirmed that these 16 isolates of Trypanosoma spp. were closely related to Trypanasoma lewisi. Conclusions: Molecular detection using PCR is as effective as conventional light microscopy analysis. This study confirms that trypanosomal infection in rodents is still high; therefore, fleas as their vectors need to be controlled in order to prevent transmission to humans. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 12 10 457 |
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ftdoajarticles |
language |
English |
topic |
trypanosoma spp polymerase chain reaction phylogenetic analysis zoonoses bandicota indica Arctic medicine. Tropical medicine RC955-962 |
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trypanosoma spp polymerase chain reaction phylogenetic analysis zoonoses bandicota indica Arctic medicine. Tropical medicine RC955-962 Phuangphet Waree Molee Natthiya Sakulsak Somchai Saengamnatdej Detection of Trypanosoma spp. in Bandicota indica from the Thai-Myanmar border area, Mae Sot District Tak Province, Thailand |
topic_facet |
trypanosoma spp polymerase chain reaction phylogenetic analysis zoonoses bandicota indica Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To investigate the prevalence of trypanosome infection and their phylogeny in Bandicota indica rats from the cadmium-contaminated area of Mae Sot and the Myanmar border. Methods: Blood samples were taken from 100 animals, and parasite infection was examined by light microscopy observation and polymerase chain reaction (PCR) studies. Results: Trypanosoma spp. infection was found in 20% of the thin blood smear samples. PCR showed positive 623 bp DNA bands in 21 samples (21%). The sequencing analysis showed that all of the samples (100%) had the Trypanasoma lewisi 18S ribosomal RNA gene. Phylogenetic analysis confirmed that these 16 isolates of Trypanosoma spp. were closely related to Trypanasoma lewisi. Conclusions: Molecular detection using PCR is as effective as conventional light microscopy analysis. This study confirms that trypanosomal infection in rodents is still high; therefore, fleas as their vectors need to be controlled in order to prevent transmission to humans. |
format |
Article in Journal/Newspaper |
author |
Phuangphet Waree Molee Natthiya Sakulsak Somchai Saengamnatdej |
author_facet |
Phuangphet Waree Molee Natthiya Sakulsak Somchai Saengamnatdej |
author_sort |
Phuangphet Waree Molee |
title |
Detection of Trypanosoma spp. in Bandicota indica from the Thai-Myanmar border area, Mae Sot District Tak Province, Thailand |
title_short |
Detection of Trypanosoma spp. in Bandicota indica from the Thai-Myanmar border area, Mae Sot District Tak Province, Thailand |
title_full |
Detection of Trypanosoma spp. in Bandicota indica from the Thai-Myanmar border area, Mae Sot District Tak Province, Thailand |
title_fullStr |
Detection of Trypanosoma spp. in Bandicota indica from the Thai-Myanmar border area, Mae Sot District Tak Province, Thailand |
title_full_unstemmed |
Detection of Trypanosoma spp. in Bandicota indica from the Thai-Myanmar border area, Mae Sot District Tak Province, Thailand |
title_sort |
detection of trypanosoma spp. in bandicota indica from the thai-myanmar border area, mae sot district tak province, thailand |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/1995-7645.269907 https://doaj.org/article/f5c4e2e68e6e4dc3a7cc66aa33055540 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 12, Iss 10, Pp 457-462 (2019) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=10;spage=457;epage=462;aulast=Molee https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.269907 https://doaj.org/article/f5c4e2e68e6e4dc3a7cc66aa33055540 |
op_doi |
https://doi.org/10.4103/1995-7645.269907 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
12 |
container_issue |
10 |
container_start_page |
457 |
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1766337034114301952 |