Antidiabetic effect of combination of fractionated-extracts of Andrographis paniculata and Centella asiatica: In vitro study
Objective: To examine the effect of combination of Andrographis paniculata herb fraction (AHF) and Centella asiatica herb fraction (CHF) on PPARγ and GLUT4 mRNA expressions in 3T3-L1 adipocyte, and its effect on insulin-stimulated glucose uptake. Methods: 3T3-L1 adipocyte cells were used to investig...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:f5b592c68833491a9450426710727381 2023-05-15T15:07:11+02:00 Antidiabetic effect of combination of fractionated-extracts of Andrographis paniculata and Centella asiatica: In vitro study LOM. Fitrawan R Ariastuti R R Tjandrawinata A E Nugroho S Pramono 2018-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.245957 https://doaj.org/article/f5b592c68833491a9450426710727381 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=11;spage=527;epage=532;aulast=Fitrawan https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.245957 https://doaj.org/article/f5b592c68833491a9450426710727381 Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 11, Pp 527-532 (2018) andrographis paniculata centella asiatica glut4 pparγ 3t3-l1 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2018 ftdoajarticles https://doi.org/10.4103/2221-1691.245957 2022-12-31T01:23:21Z Objective: To examine the effect of combination of Andrographis paniculata herb fraction (AHF) and Centella asiatica herb fraction (CHF) on PPARγ and GLUT4 mRNA expressions in 3T3-L1 adipocyte, and its effect on insulin-stimulated glucose uptake. Methods: 3T3-L1 adipocyte cells were used to investigate gene expression of PPAR γ and GLUT4 proteins by reverse transcription-polymerase chain reaction method. The adipocyte cells were differentiated by using insulin, dexamethasone and 3-isobutyl-1-methylxanthine from 3T3-L1 cells. Pioglitazone, AHF, CHF and the combination of both herbs were evaluated on glucose uptake activity, PPARγ and GLUT4 mRNA expressions in 3T3-L1 adipocyte. Results: The results showed that combination of AHF at 30 μg/mL and CHF at 10 μg/mL could enhance insulin-stimulated glucose uptake. The combination also increased PPARγ and GLUT4 mRNA expressions significantly in comparison to those of negative control (DMSO). These effects were equal in comparison to those of pioglitazone (0.02 μM) and its single extracts. Conclusions: The combination of AHF and CHF can increase glucose uptake and insulin sensitivity through up-regulation of PPARγ and GLUT4 mRNA expressions in 3T3-L1 adipocyte. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 8 11 527 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
andrographis paniculata centella asiatica glut4 pparγ 3t3-l1 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
andrographis paniculata centella asiatica glut4 pparγ 3t3-l1 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 LOM. Fitrawan R Ariastuti R R Tjandrawinata A E Nugroho S Pramono Antidiabetic effect of combination of fractionated-extracts of Andrographis paniculata and Centella asiatica: In vitro study |
topic_facet |
andrographis paniculata centella asiatica glut4 pparγ 3t3-l1 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To examine the effect of combination of Andrographis paniculata herb fraction (AHF) and Centella asiatica herb fraction (CHF) on PPARγ and GLUT4 mRNA expressions in 3T3-L1 adipocyte, and its effect on insulin-stimulated glucose uptake. Methods: 3T3-L1 adipocyte cells were used to investigate gene expression of PPAR γ and GLUT4 proteins by reverse transcription-polymerase chain reaction method. The adipocyte cells were differentiated by using insulin, dexamethasone and 3-isobutyl-1-methylxanthine from 3T3-L1 cells. Pioglitazone, AHF, CHF and the combination of both herbs were evaluated on glucose uptake activity, PPARγ and GLUT4 mRNA expressions in 3T3-L1 adipocyte. Results: The results showed that combination of AHF at 30 μg/mL and CHF at 10 μg/mL could enhance insulin-stimulated glucose uptake. The combination also increased PPARγ and GLUT4 mRNA expressions significantly in comparison to those of negative control (DMSO). These effects were equal in comparison to those of pioglitazone (0.02 μM) and its single extracts. Conclusions: The combination of AHF and CHF can increase glucose uptake and insulin sensitivity through up-regulation of PPARγ and GLUT4 mRNA expressions in 3T3-L1 adipocyte. |
format |
Article in Journal/Newspaper |
author |
LOM. Fitrawan R Ariastuti R R Tjandrawinata A E Nugroho S Pramono |
author_facet |
LOM. Fitrawan R Ariastuti R R Tjandrawinata A E Nugroho S Pramono |
author_sort |
LOM. Fitrawan |
title |
Antidiabetic effect of combination of fractionated-extracts of Andrographis paniculata and Centella asiatica: In vitro study |
title_short |
Antidiabetic effect of combination of fractionated-extracts of Andrographis paniculata and Centella asiatica: In vitro study |
title_full |
Antidiabetic effect of combination of fractionated-extracts of Andrographis paniculata and Centella asiatica: In vitro study |
title_fullStr |
Antidiabetic effect of combination of fractionated-extracts of Andrographis paniculata and Centella asiatica: In vitro study |
title_full_unstemmed |
Antidiabetic effect of combination of fractionated-extracts of Andrographis paniculata and Centella asiatica: In vitro study |
title_sort |
antidiabetic effect of combination of fractionated-extracts of andrographis paniculata and centella asiatica: in vitro study |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/2221-1691.245957 https://doaj.org/article/f5b592c68833491a9450426710727381 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 11, Pp 527-532 (2018) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=11;spage=527;epage=532;aulast=Fitrawan https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.245957 https://doaj.org/article/f5b592c68833491a9450426710727381 |
op_doi |
https://doi.org/10.4103/2221-1691.245957 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
8 |
container_issue |
11 |
container_start_page |
527 |
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1766338740925497344 |