Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate Reatividade de anticorpos de soros humanos a antígenos de Paracoccidioides brasiliensis tratados com metaperiodato sódico
In this study, we evaluated the profile of anti-Paracoccidioides brasiliensis immunoglobulin isotypes in serum from patients with the acute and chronic forms of paracoccidioidomycosis, using the whole Paracoccidioides brasiliensis antigen and the antigen treated with sodium metaperiodate. All the im...
Published in: | Revista da Sociedade Brasileira de Medicina Tropical |
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Sociedade Brasileira de Medicina Tropical (SBMT)
2008
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ftdoajarticles:oai:doaj.org/article:f5b56dd4e6ce4a86bf8bb65a126f29dd 2023-05-15T15:17:32+02:00 Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate Reatividade de anticorpos de soros humanos a antígenos de Paracoccidioides brasiliensis tratados com metaperiodato sódico Ana Paula Ferreira Thadeu Côrrea Rosângela Cunha Marcos José Marques Maria Angela Montesano Maria Aparecida Souza Henrique Couto Teixeira 2008-08-01T00:00:00Z https://doi.org/10.1590/S0037-86822008000400001 https://doaj.org/article/f5b56dd4e6ce4a86bf8bb65a126f29dd EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822008000400001 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 doi:10.1590/S0037-86822008000400001 0037-8682 1678-9849 https://doaj.org/article/f5b56dd4e6ce4a86bf8bb65a126f29dd Revista da Sociedade Brasileira de Medicina Tropical, Vol 41, Iss 4, Pp 325-329 (2008) Paracoccidiodomicose Antígenos Metaperiodato Diagnóstico Paracoccidioidomycosis Antigens Metaperiodate Diagnosis Arctic medicine. Tropical medicine RC955-962 article 2008 ftdoajarticles https://doi.org/10.1590/S0037-86822008000400001 2022-12-30T21:27:33Z In this study, we evaluated the profile of anti-Paracoccidioides brasiliensis immunoglobulin isotypes in serum from patients with the acute and chronic forms of paracoccidioidomycosis, using the whole Paracoccidioides brasiliensis antigen and the antigen treated with sodium metaperiodate. All the immunoglobulin isotypes present in the serum from patients with the acute and chronic forms of paracoccidioidomycosis presented higher reactivity towards the whole antigen than to the antigen treated with metaperiodate (P < 0.05). The reactivity of IgG and IgM to the antigen treated with metaperiodate was greater in serum from patients with the acute form of the disease (P < 0.05), while IgA was more reactive in serum from patients with the chronic form (P < 0.05). There was greater reactivity of IgG1 and IgG2 to the whole antigen and the antigen treated with metaperiodate in the serum from patients with paracoccidioidomycosis than there was in serum from patients with other parasitic infections (P < 0.05). Furthermore, IgG1 from patients with the acute form recognized the 19kDa, 27kDa and 31kDa antigens in the western blot test. Thus, the results suggest that modifications to the epitopes of Paracoccidioides brasiliensis antigens may help to improve the immunodiagnosis of paracoccidioidomycosis. Neste trabalho, foi avaliado o perfil de isotipos de imunoglobulinas anti-Paracoccidioides brasiliensis em soros de pacientes com formas crônica e aguda de paracoccidiodomicoses usando antígeno total e tratado com meta-periodato. Todos os tipos de imunoglobulinas presentes nos soros de pacientes com formas aguda e crônica apresentaram alta reatividade ao antígeno total quando comparado ao tratado com meta-periodato (P < 0,05). Houve maior reatividade de IgG e IgM anti-antígeno tratado com meta-periodato em soros de pacientes com forma aguda da doença (P < 0,05), enquanto IgA foi mais reativa em soros da forma crônica (P < 0,05). Houve maior reatividade de IgG1 e IgG2 com antígeno total e tratado com ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Alta Aguda ENVELOPE(-26.750,-26.750,-76.150,-76.150) Revista da Sociedade Brasileira de Medicina Tropical 41 4 325 329 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Paracoccidiodomicose Antígenos Metaperiodato Diagnóstico Paracoccidioidomycosis Antigens Metaperiodate Diagnosis Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Paracoccidiodomicose Antígenos Metaperiodato Diagnóstico Paracoccidioidomycosis Antigens Metaperiodate Diagnosis Arctic medicine. Tropical medicine RC955-962 Ana Paula Ferreira Thadeu Côrrea Rosângela Cunha Marcos José Marques Maria Angela Montesano Maria Aparecida Souza Henrique Couto Teixeira Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate Reatividade de anticorpos de soros humanos a antígenos de Paracoccidioides brasiliensis tratados com metaperiodato sódico |
topic_facet |
Paracoccidiodomicose Antígenos Metaperiodato Diagnóstico Paracoccidioidomycosis Antigens Metaperiodate Diagnosis Arctic medicine. Tropical medicine RC955-962 |
description |
In this study, we evaluated the profile of anti-Paracoccidioides brasiliensis immunoglobulin isotypes in serum from patients with the acute and chronic forms of paracoccidioidomycosis, using the whole Paracoccidioides brasiliensis antigen and the antigen treated with sodium metaperiodate. All the immunoglobulin isotypes present in the serum from patients with the acute and chronic forms of paracoccidioidomycosis presented higher reactivity towards the whole antigen than to the antigen treated with metaperiodate (P < 0.05). The reactivity of IgG and IgM to the antigen treated with metaperiodate was greater in serum from patients with the acute form of the disease (P < 0.05), while IgA was more reactive in serum from patients with the chronic form (P < 0.05). There was greater reactivity of IgG1 and IgG2 to the whole antigen and the antigen treated with metaperiodate in the serum from patients with paracoccidioidomycosis than there was in serum from patients with other parasitic infections (P < 0.05). Furthermore, IgG1 from patients with the acute form recognized the 19kDa, 27kDa and 31kDa antigens in the western blot test. Thus, the results suggest that modifications to the epitopes of Paracoccidioides brasiliensis antigens may help to improve the immunodiagnosis of paracoccidioidomycosis. Neste trabalho, foi avaliado o perfil de isotipos de imunoglobulinas anti-Paracoccidioides brasiliensis em soros de pacientes com formas crônica e aguda de paracoccidiodomicoses usando antígeno total e tratado com meta-periodato. Todos os tipos de imunoglobulinas presentes nos soros de pacientes com formas aguda e crônica apresentaram alta reatividade ao antígeno total quando comparado ao tratado com meta-periodato (P < 0,05). Houve maior reatividade de IgG e IgM anti-antígeno tratado com meta-periodato em soros de pacientes com forma aguda da doença (P < 0,05), enquanto IgA foi mais reativa em soros da forma crônica (P < 0,05). Houve maior reatividade de IgG1 e IgG2 com antígeno total e tratado com ... |
format |
Article in Journal/Newspaper |
author |
Ana Paula Ferreira Thadeu Côrrea Rosângela Cunha Marcos José Marques Maria Angela Montesano Maria Aparecida Souza Henrique Couto Teixeira |
author_facet |
Ana Paula Ferreira Thadeu Côrrea Rosângela Cunha Marcos José Marques Maria Angela Montesano Maria Aparecida Souza Henrique Couto Teixeira |
author_sort |
Ana Paula Ferreira |
title |
Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate Reatividade de anticorpos de soros humanos a antígenos de Paracoccidioides brasiliensis tratados com metaperiodato sódico |
title_short |
Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate Reatividade de anticorpos de soros humanos a antígenos de Paracoccidioides brasiliensis tratados com metaperiodato sódico |
title_full |
Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate Reatividade de anticorpos de soros humanos a antígenos de Paracoccidioides brasiliensis tratados com metaperiodato sódico |
title_fullStr |
Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate Reatividade de anticorpos de soros humanos a antígenos de Paracoccidioides brasiliensis tratados com metaperiodato sódico |
title_full_unstemmed |
Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate Reatividade de anticorpos de soros humanos a antígenos de Paracoccidioides brasiliensis tratados com metaperiodato sódico |
title_sort |
human serum antibody reactivity towards paracoccidioides brasiliensis antigens treated with sodium metaperiodate reatividade de anticorpos de soros humanos a antígenos de paracoccidioides brasiliensis tratados com metaperiodato sódico |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2008 |
url |
https://doi.org/10.1590/S0037-86822008000400001 https://doaj.org/article/f5b56dd4e6ce4a86bf8bb65a126f29dd |
long_lat |
ENVELOPE(-26.750,-26.750,-76.150,-76.150) |
geographic |
Arctic Alta Aguda |
geographic_facet |
Arctic Alta Aguda |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 41, Iss 4, Pp 325-329 (2008) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822008000400001 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 doi:10.1590/S0037-86822008000400001 0037-8682 1678-9849 https://doaj.org/article/f5b56dd4e6ce4a86bf8bb65a126f29dd |
op_doi |
https://doi.org/10.1590/S0037-86822008000400001 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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41 |
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325 |
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329 |
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