Temporal changes in pathology and viral RNA distribution in guinea pigs following separate infection with two New World Arenaviruses.
Numerous arenaviruses have been identified throughout the Americas and a subset of these viruses cause viral hemorrhagic fever in humans. This study compared the pathology and viral RNA distribution in Hartley guinea pigs challenged with two human-disease causing New World arenaviruses, Junin virus...
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ftdoajarticles:oai:doaj.org/article:f59f6fbec6614cfcbee25bdb996bc623 2023-11-05T03:39:50+01:00 Temporal changes in pathology and viral RNA distribution in guinea pigs following separate infection with two New World Arenaviruses. Curtis Cline Xiankun Zeng Todd M Bell Carl Shaia Paul Facemire Janice Williams Neil Davis April Babka Edwin Picado Colin Fitzpatrick Joseph W Golden 2023-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011620 https://doaj.org/article/f59f6fbec6614cfcbee25bdb996bc623 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0011620 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011620 https://doaj.org/article/f59f6fbec6614cfcbee25bdb996bc623 PLoS Neglected Tropical Diseases, Vol 17, Iss 9, p e0011620 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011620 2023-10-08T00:37:57Z Numerous arenaviruses have been identified throughout the Americas and a subset of these viruses cause viral hemorrhagic fever in humans. This study compared the pathology and viral RNA distribution in Hartley guinea pigs challenged with two human-disease causing New World arenaviruses, Junin virus (JUNV) or Guanarito virus (GTOV). Histopathologic analysis and RNA in situ hybridization revealed similar pathology and viral RNA distribution for both groups of animals challenged with either JUNV or GTOV on days 3, 7, 10 and 12 post exposure (PE). Gross lesions were first observed on day 7 and primarily involved the lungs and liver. The most severe histologic lesions occurred in the lymph nodes, spleen, and thymus and included lymphoid depletion and necrosis which increased in severity over time. Extensive necrosis was also observed in the bone marrow on day 12. Minimal to mild inflammation with and without necrosis was observed in the choroid plexus of the brain, choroid of the eye, intestinal tract, lung and adrenal gland. Significant liver lesions were rare, consisting predominantly of hepatocyte vacuolation. Viral RNA labeling was identified in nearly all organs examined, was often extensive in certain organs and generally increased over time starting on day 7. Our data demonstrate the guinea pig may serve as a useful model to study New World arenavirus infection in humans and for the evaluation and development of medical countermeasures. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles PLOS Neglected Tropical Diseases 17 9 e0011620 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Curtis Cline Xiankun Zeng Todd M Bell Carl Shaia Paul Facemire Janice Williams Neil Davis April Babka Edwin Picado Colin Fitzpatrick Joseph W Golden Temporal changes in pathology and viral RNA distribution in guinea pigs following separate infection with two New World Arenaviruses. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Numerous arenaviruses have been identified throughout the Americas and a subset of these viruses cause viral hemorrhagic fever in humans. This study compared the pathology and viral RNA distribution in Hartley guinea pigs challenged with two human-disease causing New World arenaviruses, Junin virus (JUNV) or Guanarito virus (GTOV). Histopathologic analysis and RNA in situ hybridization revealed similar pathology and viral RNA distribution for both groups of animals challenged with either JUNV or GTOV on days 3, 7, 10 and 12 post exposure (PE). Gross lesions were first observed on day 7 and primarily involved the lungs and liver. The most severe histologic lesions occurred in the lymph nodes, spleen, and thymus and included lymphoid depletion and necrosis which increased in severity over time. Extensive necrosis was also observed in the bone marrow on day 12. Minimal to mild inflammation with and without necrosis was observed in the choroid plexus of the brain, choroid of the eye, intestinal tract, lung and adrenal gland. Significant liver lesions were rare, consisting predominantly of hepatocyte vacuolation. Viral RNA labeling was identified in nearly all organs examined, was often extensive in certain organs and generally increased over time starting on day 7. Our data demonstrate the guinea pig may serve as a useful model to study New World arenavirus infection in humans and for the evaluation and development of medical countermeasures. |
format |
Article in Journal/Newspaper |
author |
Curtis Cline Xiankun Zeng Todd M Bell Carl Shaia Paul Facemire Janice Williams Neil Davis April Babka Edwin Picado Colin Fitzpatrick Joseph W Golden |
author_facet |
Curtis Cline Xiankun Zeng Todd M Bell Carl Shaia Paul Facemire Janice Williams Neil Davis April Babka Edwin Picado Colin Fitzpatrick Joseph W Golden |
author_sort |
Curtis Cline |
title |
Temporal changes in pathology and viral RNA distribution in guinea pigs following separate infection with two New World Arenaviruses. |
title_short |
Temporal changes in pathology and viral RNA distribution in guinea pigs following separate infection with two New World Arenaviruses. |
title_full |
Temporal changes in pathology and viral RNA distribution in guinea pigs following separate infection with two New World Arenaviruses. |
title_fullStr |
Temporal changes in pathology and viral RNA distribution in guinea pigs following separate infection with two New World Arenaviruses. |
title_full_unstemmed |
Temporal changes in pathology and viral RNA distribution in guinea pigs following separate infection with two New World Arenaviruses. |
title_sort |
temporal changes in pathology and viral rna distribution in guinea pigs following separate infection with two new world arenaviruses. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pntd.0011620 https://doaj.org/article/f59f6fbec6614cfcbee25bdb996bc623 |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 9, p e0011620 (2023) |
op_relation |
https://doi.org/10.1371/journal.pntd.0011620 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011620 https://doaj.org/article/f59f6fbec6614cfcbee25bdb996bc623 |
op_doi |
https://doi.org/10.1371/journal.pntd.0011620 |
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PLOS Neglected Tropical Diseases |
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17 |
container_issue |
9 |
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e0011620 |
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1781695753832366080 |