Syzygium aromaticum ethanol extract reduces AlCl3-induced neurotoxicity in mice brain through regulation of amyloid precursor protein and oxidative stress gene expression
Objective: To investigate the neuroprotective effects of Syzygium aromaticum (S. aromaticum) extract (500 mg/kg) on AlCl3 (300 mg/kg)-induced mouse model of oxidative stress and neurotoxicity. Methods: An ethanolic extract of S. aromaticum seeds was prepared and the active compounds were identified...
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ftdoajarticles:oai:doaj.org/article:f525838745794df4a5463052f516f583 2023-05-15T15:09:40+02:00 Syzygium aromaticum ethanol extract reduces AlCl3-induced neurotoxicity in mice brain through regulation of amyloid precursor protein and oxidative stress gene expression Sanila Amber Syed Adnan Ali Shah Touqeer Ahmed Saadia Zahid 2018-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.225019 https://doaj.org/article/f525838745794df4a5463052f516f583 EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=2;spage=123;epage=130;aulast=Amber https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.225019 https://doaj.org/article/f525838745794df4a5463052f516f583 Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 2, Pp 123-130 (2018) amyloid precursor protein oxidative stress aluminum neurodegeneration Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.4103/1995-7645.225019 2022-12-31T01:36:41Z Objective: To investigate the neuroprotective effects of Syzygium aromaticum (S. aromaticum) extract (500 mg/kg) on AlCl3 (300 mg/kg)-induced mouse model of oxidative stress and neurotoxicity. Methods: An ethanolic extract of S. aromaticum seeds was prepared and the active compounds were identified using nuclear magnetic resonance spectroscopy. BALB/ c mice were divided into five groups (negative control, AlCl3-treated, self-recovery, AlCl3 + S. aromaticum, S. aromaticum only; n=10) and treated with AlCl3 and S. aromaticum extract. Expression of oxidative markers [Superoxide dismutase 1 (SOD1) and peroxiredoxin 6 (Prdx6)] and amyloid precursor protein (APP) in the hippocampus and cortex was evaluated via PCR. Histopathological assessment was performed to investigate the extent of neurodegeneration. Results: It was observed that AlCl3 exposure increased the expression of APP770 while simultaneously down regulated the expression of APP695. AlCl3 also induced a significant decrease (P<0.05) and an increase (P<0.05) in the expression level of SOD1 and Prdx6, respectively. A substantial decrease substantial (P<0.05) in the density of Nissl substance was also observed in cortex of the mice treated with AlCl3. Interestingly, treatment with S. aromaticum extract normalized the alterations in the expression level of SOD1, Prdx6 and APP isoforms and improved the neuronal structural damage. Conclusions: The results showed that S. aromaticum is a promising antioxidant and a neuroprotective agent. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 11 2 123 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
amyloid precursor protein oxidative stress aluminum neurodegeneration Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
amyloid precursor protein oxidative stress aluminum neurodegeneration Arctic medicine. Tropical medicine RC955-962 Sanila Amber Syed Adnan Ali Shah Touqeer Ahmed Saadia Zahid Syzygium aromaticum ethanol extract reduces AlCl3-induced neurotoxicity in mice brain through regulation of amyloid precursor protein and oxidative stress gene expression |
topic_facet |
amyloid precursor protein oxidative stress aluminum neurodegeneration Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To investigate the neuroprotective effects of Syzygium aromaticum (S. aromaticum) extract (500 mg/kg) on AlCl3 (300 mg/kg)-induced mouse model of oxidative stress and neurotoxicity. Methods: An ethanolic extract of S. aromaticum seeds was prepared and the active compounds were identified using nuclear magnetic resonance spectroscopy. BALB/ c mice were divided into five groups (negative control, AlCl3-treated, self-recovery, AlCl3 + S. aromaticum, S. aromaticum only; n=10) and treated with AlCl3 and S. aromaticum extract. Expression of oxidative markers [Superoxide dismutase 1 (SOD1) and peroxiredoxin 6 (Prdx6)] and amyloid precursor protein (APP) in the hippocampus and cortex was evaluated via PCR. Histopathological assessment was performed to investigate the extent of neurodegeneration. Results: It was observed that AlCl3 exposure increased the expression of APP770 while simultaneously down regulated the expression of APP695. AlCl3 also induced a significant decrease (P<0.05) and an increase (P<0.05) in the expression level of SOD1 and Prdx6, respectively. A substantial decrease substantial (P<0.05) in the density of Nissl substance was also observed in cortex of the mice treated with AlCl3. Interestingly, treatment with S. aromaticum extract normalized the alterations in the expression level of SOD1, Prdx6 and APP isoforms and improved the neuronal structural damage. Conclusions: The results showed that S. aromaticum is a promising antioxidant and a neuroprotective agent. |
format |
Article in Journal/Newspaper |
author |
Sanila Amber Syed Adnan Ali Shah Touqeer Ahmed Saadia Zahid |
author_facet |
Sanila Amber Syed Adnan Ali Shah Touqeer Ahmed Saadia Zahid |
author_sort |
Sanila Amber |
title |
Syzygium aromaticum ethanol extract reduces AlCl3-induced neurotoxicity in mice brain through regulation of amyloid precursor protein and oxidative stress gene expression |
title_short |
Syzygium aromaticum ethanol extract reduces AlCl3-induced neurotoxicity in mice brain through regulation of amyloid precursor protein and oxidative stress gene expression |
title_full |
Syzygium aromaticum ethanol extract reduces AlCl3-induced neurotoxicity in mice brain through regulation of amyloid precursor protein and oxidative stress gene expression |
title_fullStr |
Syzygium aromaticum ethanol extract reduces AlCl3-induced neurotoxicity in mice brain through regulation of amyloid precursor protein and oxidative stress gene expression |
title_full_unstemmed |
Syzygium aromaticum ethanol extract reduces AlCl3-induced neurotoxicity in mice brain through regulation of amyloid precursor protein and oxidative stress gene expression |
title_sort |
syzygium aromaticum ethanol extract reduces alcl3-induced neurotoxicity in mice brain through regulation of amyloid precursor protein and oxidative stress gene expression |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/1995-7645.225019 https://doaj.org/article/f525838745794df4a5463052f516f583 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 2, Pp 123-130 (2018) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=2;spage=123;epage=130;aulast=Amber https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.225019 https://doaj.org/article/f525838745794df4a5463052f516f583 |
op_doi |
https://doi.org/10.4103/1995-7645.225019 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
11 |
container_issue |
2 |
container_start_page |
123 |
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1766340816660332544 |