Selective activation of TCR- γδ + cells in endemic Burkitt's lymphoma
Abstract Background The overlap in geographical distribution of Plasmodium falciparum malaria and endemic Burkitt's lymphoma (eBL) – an aggressive Epstein-Barr virus (EBV)-associated B-cell tumour occurring almost exclusively in the tropics – strongly suggests a link between the two diseases. I...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2007
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1475-2875-6-69 https://doaj.org/article/f4e57bf1650c47db8916beaa941a94ca |
id |
ftdoajarticles:oai:doaj.org/article:f4e57bf1650c47db8916beaa941a94ca |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:f4e57bf1650c47db8916beaa941a94ca 2023-05-15T15:16:10+02:00 Selective activation of TCR- γδ + cells in endemic Burkitt's lymphoma Hviid Lars Tetteh John Welbeck Jennifer E Futagbi Godfred Akanmori Bartholomew D 2007-05-01T00:00:00Z https://doi.org/10.1186/1475-2875-6-69 https://doaj.org/article/f4e57bf1650c47db8916beaa941a94ca EN eng BMC http://www.malariajournal.com/content/6/1/69 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-6-69 1475-2875 https://doaj.org/article/f4e57bf1650c47db8916beaa941a94ca Malaria Journal, Vol 6, Iss 1, p 69 (2007) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2007 ftdoajarticles https://doi.org/10.1186/1475-2875-6-69 2022-12-31T01:09:53Z Abstract Background The overlap in geographical distribution of Plasmodium falciparum malaria and endemic Burkitt's lymphoma (eBL) – an aggressive Epstein-Barr virus (EBV)-associated B-cell tumour occurring almost exclusively in the tropics – strongly suggests a link between the two diseases. It is suspected that the polyclonal B-cell activation in P. falciparum malaria may precipitate a breakdown in homeostatic T-cell control of EBV-immortalized B-cell proliferation. Previous studies have suggested that a particular T-cell subset, characterized by expression of V δ 1 + γδ T-cell receptors, is important for maintaining B-cell homeostasis, both in P. falciparum - exposed populations and in individuals subject to polyclonal B-cell activation of other aetiology. The objective of the present study was, therefore, to characterize lymphocyte phenotypes and to investigate possible differences in T-cell subset composition and activation status in P. falciparum -exposed Ghanaian children with and without eBL. Methods Venous blood samples in heparin from 21 eBL patients (mean age: 7.0 years; range: 3–11 years), referred to the Burkitt's Tumour Centre at Korle-Bu Teaching Hospital, Accra and 15 healthy, age and sex matched children, were stained with fluorescein isothiocyanate (FITC)-, phycoerythrin (PE)-, R-phycoerythrin (RPE)- and RPE-Cy5-conjugated antibodies (CD3, CD4, CD8, CD25, CD69, CD95, HLA-DR, TCR- γδ , V δ 1, V δ 3, V γ 9 and B-cells) and acquired on a flow cytometer. Results A reduction in the proportion of CD3 + cells in eBL patients, due mainly to perturbations among TCR- γδ + cells was observed. In contrast, the proportions of CD4 + or CD8 + cells were relatively unaffected, as were the mean numbers of peripheral blood mononuclear cells. Conclusion Selective changes in numbers and activation status of TCR- γδ + cells occurs in Ghanaian children with eBL, a pattern which is similar to P. falciparum -induced changes. The data supports the hypothesis of a regulatory role for V δ 1 + TcR- γδ T-cells in ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 6 1 69 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Hviid Lars Tetteh John Welbeck Jennifer E Futagbi Godfred Akanmori Bartholomew D Selective activation of TCR- γδ + cells in endemic Burkitt's lymphoma |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The overlap in geographical distribution of Plasmodium falciparum malaria and endemic Burkitt's lymphoma (eBL) – an aggressive Epstein-Barr virus (EBV)-associated B-cell tumour occurring almost exclusively in the tropics – strongly suggests a link between the two diseases. It is suspected that the polyclonal B-cell activation in P. falciparum malaria may precipitate a breakdown in homeostatic T-cell control of EBV-immortalized B-cell proliferation. Previous studies have suggested that a particular T-cell subset, characterized by expression of V δ 1 + γδ T-cell receptors, is important for maintaining B-cell homeostasis, both in P. falciparum - exposed populations and in individuals subject to polyclonal B-cell activation of other aetiology. The objective of the present study was, therefore, to characterize lymphocyte phenotypes and to investigate possible differences in T-cell subset composition and activation status in P. falciparum -exposed Ghanaian children with and without eBL. Methods Venous blood samples in heparin from 21 eBL patients (mean age: 7.0 years; range: 3–11 years), referred to the Burkitt's Tumour Centre at Korle-Bu Teaching Hospital, Accra and 15 healthy, age and sex matched children, were stained with fluorescein isothiocyanate (FITC)-, phycoerythrin (PE)-, R-phycoerythrin (RPE)- and RPE-Cy5-conjugated antibodies (CD3, CD4, CD8, CD25, CD69, CD95, HLA-DR, TCR- γδ , V δ 1, V δ 3, V γ 9 and B-cells) and acquired on a flow cytometer. Results A reduction in the proportion of CD3 + cells in eBL patients, due mainly to perturbations among TCR- γδ + cells was observed. In contrast, the proportions of CD4 + or CD8 + cells were relatively unaffected, as were the mean numbers of peripheral blood mononuclear cells. Conclusion Selective changes in numbers and activation status of TCR- γδ + cells occurs in Ghanaian children with eBL, a pattern which is similar to P. falciparum -induced changes. The data supports the hypothesis of a regulatory role for V δ 1 + TcR- γδ T-cells in ... |
format |
Article in Journal/Newspaper |
author |
Hviid Lars Tetteh John Welbeck Jennifer E Futagbi Godfred Akanmori Bartholomew D |
author_facet |
Hviid Lars Tetteh John Welbeck Jennifer E Futagbi Godfred Akanmori Bartholomew D |
author_sort |
Hviid Lars |
title |
Selective activation of TCR- γδ + cells in endemic Burkitt's lymphoma |
title_short |
Selective activation of TCR- γδ + cells in endemic Burkitt's lymphoma |
title_full |
Selective activation of TCR- γδ + cells in endemic Burkitt's lymphoma |
title_fullStr |
Selective activation of TCR- γδ + cells in endemic Burkitt's lymphoma |
title_full_unstemmed |
Selective activation of TCR- γδ + cells in endemic Burkitt's lymphoma |
title_sort |
selective activation of tcr- γδ + cells in endemic burkitt's lymphoma |
publisher |
BMC |
publishDate |
2007 |
url |
https://doi.org/10.1186/1475-2875-6-69 https://doaj.org/article/f4e57bf1650c47db8916beaa941a94ca |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 6, Iss 1, p 69 (2007) |
op_relation |
http://www.malariajournal.com/content/6/1/69 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-6-69 1475-2875 https://doaj.org/article/f4e57bf1650c47db8916beaa941a94ca |
op_doi |
https://doi.org/10.1186/1475-2875-6-69 |
container_title |
Malaria Journal |
container_volume |
6 |
container_issue |
1 |
container_start_page |
69 |
_version_ |
1766346465904427008 |