Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica)
In this study, for the first time, we report the identification and characterization of culturable fast-growing bacteria isolated from the sea-affected temporary meltwater ponds (MPs) in the East Antarctica area of the Vecherny region (−67.656317, 46.175058) of the Thala Hills Oasis, Enderby Land. W...
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2022
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ftdoajarticles:oai:doaj.org/article:f48a0ef4e241452cba3e0371a8fba6af 2023-08-27T04:04:56+02:00 Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica) Volha Akulava Uladzislau Miamin Katsiaryna Akhremchuk Leonid Valentovich Andrey Dolgikh Volha Shapaval 2022-07-01T00:00:00Z https://doi.org/10.3390/biology11081143 https://doaj.org/article/f48a0ef4e241452cba3e0371a8fba6af EN eng MDPI AG https://www.mdpi.com/2079-7737/11/8/1143 https://doaj.org/toc/2079-7737 doi:10.3390/biology11081143 2079-7737 https://doaj.org/article/f48a0ef4e241452cba3e0371a8fba6af Biology, Vol 11, Iss 1143, p 1143 (2022) meltwater ponds Antarctic bacteria 16S rRNA gene sequencing plate-based assays thermotolerance enzymatic activity Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.3390/biology11081143 2023-08-06T00:44:19Z In this study, for the first time, we report the identification and characterization of culturable fast-growing bacteria isolated from the sea-affected temporary meltwater ponds (MPs) in the East Antarctica area of the Vecherny region (−67.656317, 46.175058) of the Thala Hills Oasis, Enderby Land. Water samples from the studied MPs showed alkaline pH (from 8.0 to 10.1) and highly varied total dissolved solids (86–94,000 mg/L). In total, twenty-nine bacterial isolates were retrieved from the studied MPs. The phylogenetic analysis based on 16S rRNA gene sequence similarities showed that the isolated bacteria belong to the phyla Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes and the twelve genera Pseudomonas , Shewanella , Acinetobacter , Sporosarcina , Facklamia , Carnobacterium , Arthrobacter , Brachybacterium , Micrococcus , Agrococcus , Leifsonia , and Flavobacterium . Most of the isolated bacteria were psychrotrophs and showed the production of one or more extracellular enzymes. Lipolytic and proteolytic activities were more prevalent among the isolates. Five isolates from the Actinobacteria phylum and one isolate from the Bacteroidetes phylum had strong pigmentation. Antibiotic susceptibility testing revealed that most of the isolates are resistant to at least one antibiotic, and seven isolates showed multi-resistance. Article in Journal/Newspaper Antarc* Antarctic Antarctica East Antarctica Enderby Land Directory of Open Access Journals: DOAJ Articles Antarctic East Antarctica Thala Hills ENVELOPE(46.000,46.000,-67.667,-67.667) Biology 11 8 1143 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
meltwater ponds Antarctic bacteria 16S rRNA gene sequencing plate-based assays thermotolerance enzymatic activity Biology (General) QH301-705.5 |
spellingShingle |
meltwater ponds Antarctic bacteria 16S rRNA gene sequencing plate-based assays thermotolerance enzymatic activity Biology (General) QH301-705.5 Volha Akulava Uladzislau Miamin Katsiaryna Akhremchuk Leonid Valentovich Andrey Dolgikh Volha Shapaval Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica) |
topic_facet |
meltwater ponds Antarctic bacteria 16S rRNA gene sequencing plate-based assays thermotolerance enzymatic activity Biology (General) QH301-705.5 |
description |
In this study, for the first time, we report the identification and characterization of culturable fast-growing bacteria isolated from the sea-affected temporary meltwater ponds (MPs) in the East Antarctica area of the Vecherny region (−67.656317, 46.175058) of the Thala Hills Oasis, Enderby Land. Water samples from the studied MPs showed alkaline pH (from 8.0 to 10.1) and highly varied total dissolved solids (86–94,000 mg/L). In total, twenty-nine bacterial isolates were retrieved from the studied MPs. The phylogenetic analysis based on 16S rRNA gene sequence similarities showed that the isolated bacteria belong to the phyla Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes and the twelve genera Pseudomonas , Shewanella , Acinetobacter , Sporosarcina , Facklamia , Carnobacterium , Arthrobacter , Brachybacterium , Micrococcus , Agrococcus , Leifsonia , and Flavobacterium . Most of the isolated bacteria were psychrotrophs and showed the production of one or more extracellular enzymes. Lipolytic and proteolytic activities were more prevalent among the isolates. Five isolates from the Actinobacteria phylum and one isolate from the Bacteroidetes phylum had strong pigmentation. Antibiotic susceptibility testing revealed that most of the isolates are resistant to at least one antibiotic, and seven isolates showed multi-resistance. |
format |
Article in Journal/Newspaper |
author |
Volha Akulava Uladzislau Miamin Katsiaryna Akhremchuk Leonid Valentovich Andrey Dolgikh Volha Shapaval |
author_facet |
Volha Akulava Uladzislau Miamin Katsiaryna Akhremchuk Leonid Valentovich Andrey Dolgikh Volha Shapaval |
author_sort |
Volha Akulava |
title |
Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica) |
title_short |
Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica) |
title_full |
Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica) |
title_fullStr |
Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica) |
title_full_unstemmed |
Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica) |
title_sort |
isolation, physiological characterization, and antibiotic susceptibility testing of fast-growing bacteria from the sea-affected temporary meltwater ponds in the thala hills oasis (enderby land, east antarctica) |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/biology11081143 https://doaj.org/article/f48a0ef4e241452cba3e0371a8fba6af |
long_lat |
ENVELOPE(46.000,46.000,-67.667,-67.667) |
geographic |
Antarctic East Antarctica Thala Hills |
geographic_facet |
Antarctic East Antarctica Thala Hills |
genre |
Antarc* Antarctic Antarctica East Antarctica Enderby Land |
genre_facet |
Antarc* Antarctic Antarctica East Antarctica Enderby Land |
op_source |
Biology, Vol 11, Iss 1143, p 1143 (2022) |
op_relation |
https://www.mdpi.com/2079-7737/11/8/1143 https://doaj.org/toc/2079-7737 doi:10.3390/biology11081143 2079-7737 https://doaj.org/article/f48a0ef4e241452cba3e0371a8fba6af |
op_doi |
https://doi.org/10.3390/biology11081143 |
container_title |
Biology |
container_volume |
11 |
container_issue |
8 |
container_start_page |
1143 |
_version_ |
1775354687929712640 |