Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica)

In this study, for the first time, we report the identification and characterization of culturable fast-growing bacteria isolated from the sea-affected temporary meltwater ponds (MPs) in the East Antarctica area of the Vecherny region (−67.656317, 46.175058) of the Thala Hills Oasis, Enderby Land. W...

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Bibliographic Details
Published in:Biology
Main Authors: Volha Akulava, Uladzislau Miamin, Katsiaryna Akhremchuk, Leonid Valentovich, Andrey Dolgikh, Volha Shapaval
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2022
Subjects:
meltwater ponds
Antarctic bacteria
16S rRNA gene sequencing
plate-based assays
thermotolerance
enzymatic activity
Biology (General)
QH301-705.5
Antarctic
East Antarctica
Thala Hills
Antarc*
Antarctica
Enderby Land
Online Access:https://doi.org/10.3390/biology11081143
https://doaj.org/article/f48a0ef4e241452cba3e0371a8fba6af
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Summary:In this study, for the first time, we report the identification and characterization of culturable fast-growing bacteria isolated from the sea-affected temporary meltwater ponds (MPs) in the East Antarctica area of the Vecherny region (−67.656317, 46.175058) of the Thala Hills Oasis, Enderby Land. Water samples from the studied MPs showed alkaline pH (from 8.0 to 10.1) and highly varied total dissolved solids (86–94,000 mg/L). In total, twenty-nine bacterial isolates were retrieved from the studied MPs. The phylogenetic analysis based on 16S rRNA gene sequence similarities showed that the isolated bacteria belong to the phyla Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes and the twelve genera Pseudomonas , Shewanella , Acinetobacter , Sporosarcina , Facklamia , Carnobacterium , Arthrobacter , Brachybacterium , Micrococcus , Agrococcus , Leifsonia , and Flavobacterium . Most of the isolated bacteria were psychrotrophs and showed the production of one or more extracellular enzymes. Lipolytic and proteolytic activities were more prevalent among the isolates. Five isolates from the Actinobacteria phylum and one isolate from the Bacteroidetes phylum had strong pigmentation. Antibiotic susceptibility testing revealed that most of the isolates are resistant to at least one antibiotic, and seven isolates showed multi-resistance.

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