Whole Community Metatranscriptomes and Lipidomes Reveal Diverse Responses Among Antarctic Phytoplankton to Changing Ice Conditions
The transition from winter to spring represents a major shift in the basal energy source for the Antarctic marine ecosystem from lipids and other sources of stored energy to sunlight. Because sea ice imposes a strong control on the transmission of sunlight into the water column during the polar spri...
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2021
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Online Access: | https://doi.org/10.3389/fmars.2021.593566 https://doaj.org/article/f47df1b6aa5240ff91047b19a9151d02 |
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ftdoajarticles:oai:doaj.org/article:f47df1b6aa5240ff91047b19a9151d02 2023-05-15T14:02:16+02:00 Whole Community Metatranscriptomes and Lipidomes Reveal Diverse Responses Among Antarctic Phytoplankton to Changing Ice Conditions Jeff S. Bowman Benjamin A. S. Van Mooy Daniel P. Lowenstein Helen F. Fredricks Colleen M. Hansel Rebecca Gast James R. Collins Nicole Couto Hugh W. Ducklow 2021-02-01T00:00:00Z https://doi.org/10.3389/fmars.2021.593566 https://doaj.org/article/f47df1b6aa5240ff91047b19a9151d02 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmars.2021.593566/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2021.593566 https://doaj.org/article/f47df1b6aa5240ff91047b19a9151d02 Frontiers in Marine Science, Vol 8 (2021) Antarctica phytoplankton lipids metatranscriptomics Palmer LTER project Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2021 ftdoajarticles https://doi.org/10.3389/fmars.2021.593566 2022-12-31T15:54:57Z The transition from winter to spring represents a major shift in the basal energy source for the Antarctic marine ecosystem from lipids and other sources of stored energy to sunlight. Because sea ice imposes a strong control on the transmission of sunlight into the water column during the polar spring, we hypothesized that the timing of the sea ice retreat influences the timing of the transition from stored energy to photosynthesis. To test the influence of sea ice on water column microbial energy utilization we took advantage of unique sea ice conditions in Arthur Harbor, an embayment near Palmer Station on the western Antarctic Peninsula, during the 2015 spring–summer seasonal transition. Over a 5-week period we sampled water from below land-fast sea ice, in the marginal ice zone at nearby Palmer Station B, and conducted an ice removal experiment with incubations of water collected below the land-fast ice. Whole-community metatranscriptomes were paired with lipidomics to better understand how lipid production and utilization was influenced by light conditions. We identified several different phytoplankton taxa that responded similarly to light by the number of genes up-regulated, and in the transcriptional complexity of this response. We applied a principal components analysis to these data to reduce their dimensionality, revealing that each of these taxa exhibited a strikingly different pattern of gene up-regulation. By correlating the changes in lipid concentration to the first principal component of log fold-change for each taxa we could make predictions about which taxa were associated with different changes in the community lipidome. We found that genes coding for the catabolism of triacylglycerol storage lipids were expressed early on in phytoplankton associated with a Fragilariopsis kerguelensis reference transcriptome. Phytoplankton associated with a Corethron pennatum reference transcriptome occupied an adjacent niche, responding favorably to higher light conditions than F. kerguelensis. Other diatom ... Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica Sea ice Directory of Open Access Journals: DOAJ Articles Antarctic Antarctic Peninsula Arthur Harbor ENVELOPE(-64.067,-64.067,-64.767,-64.767) Palmer Station ENVELOPE(-64.050,-64.050,-64.770,-64.770) Palmer-Station ENVELOPE(-64.050,-64.050,-64.770,-64.770) The Antarctic Frontiers in Marine Science 8 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctica phytoplankton lipids metatranscriptomics Palmer LTER project Science Q General. Including nature conservation geographical distribution QH1-199.5 |
spellingShingle |
Antarctica phytoplankton lipids metatranscriptomics Palmer LTER project Science Q General. Including nature conservation geographical distribution QH1-199.5 Jeff S. Bowman Benjamin A. S. Van Mooy Daniel P. Lowenstein Helen F. Fredricks Colleen M. Hansel Rebecca Gast James R. Collins Nicole Couto Hugh W. Ducklow Whole Community Metatranscriptomes and Lipidomes Reveal Diverse Responses Among Antarctic Phytoplankton to Changing Ice Conditions |
topic_facet |
Antarctica phytoplankton lipids metatranscriptomics Palmer LTER project Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
The transition from winter to spring represents a major shift in the basal energy source for the Antarctic marine ecosystem from lipids and other sources of stored energy to sunlight. Because sea ice imposes a strong control on the transmission of sunlight into the water column during the polar spring, we hypothesized that the timing of the sea ice retreat influences the timing of the transition from stored energy to photosynthesis. To test the influence of sea ice on water column microbial energy utilization we took advantage of unique sea ice conditions in Arthur Harbor, an embayment near Palmer Station on the western Antarctic Peninsula, during the 2015 spring–summer seasonal transition. Over a 5-week period we sampled water from below land-fast sea ice, in the marginal ice zone at nearby Palmer Station B, and conducted an ice removal experiment with incubations of water collected below the land-fast ice. Whole-community metatranscriptomes were paired with lipidomics to better understand how lipid production and utilization was influenced by light conditions. We identified several different phytoplankton taxa that responded similarly to light by the number of genes up-regulated, and in the transcriptional complexity of this response. We applied a principal components analysis to these data to reduce their dimensionality, revealing that each of these taxa exhibited a strikingly different pattern of gene up-regulation. By correlating the changes in lipid concentration to the first principal component of log fold-change for each taxa we could make predictions about which taxa were associated with different changes in the community lipidome. We found that genes coding for the catabolism of triacylglycerol storage lipids were expressed early on in phytoplankton associated with a Fragilariopsis kerguelensis reference transcriptome. Phytoplankton associated with a Corethron pennatum reference transcriptome occupied an adjacent niche, responding favorably to higher light conditions than F. kerguelensis. Other diatom ... |
format |
Article in Journal/Newspaper |
author |
Jeff S. Bowman Benjamin A. S. Van Mooy Daniel P. Lowenstein Helen F. Fredricks Colleen M. Hansel Rebecca Gast James R. Collins Nicole Couto Hugh W. Ducklow |
author_facet |
Jeff S. Bowman Benjamin A. S. Van Mooy Daniel P. Lowenstein Helen F. Fredricks Colleen M. Hansel Rebecca Gast James R. Collins Nicole Couto Hugh W. Ducklow |
author_sort |
Jeff S. Bowman |
title |
Whole Community Metatranscriptomes and Lipidomes Reveal Diverse Responses Among Antarctic Phytoplankton to Changing Ice Conditions |
title_short |
Whole Community Metatranscriptomes and Lipidomes Reveal Diverse Responses Among Antarctic Phytoplankton to Changing Ice Conditions |
title_full |
Whole Community Metatranscriptomes and Lipidomes Reveal Diverse Responses Among Antarctic Phytoplankton to Changing Ice Conditions |
title_fullStr |
Whole Community Metatranscriptomes and Lipidomes Reveal Diverse Responses Among Antarctic Phytoplankton to Changing Ice Conditions |
title_full_unstemmed |
Whole Community Metatranscriptomes and Lipidomes Reveal Diverse Responses Among Antarctic Phytoplankton to Changing Ice Conditions |
title_sort |
whole community metatranscriptomes and lipidomes reveal diverse responses among antarctic phytoplankton to changing ice conditions |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doi.org/10.3389/fmars.2021.593566 https://doaj.org/article/f47df1b6aa5240ff91047b19a9151d02 |
long_lat |
ENVELOPE(-64.067,-64.067,-64.767,-64.767) ENVELOPE(-64.050,-64.050,-64.770,-64.770) ENVELOPE(-64.050,-64.050,-64.770,-64.770) |
geographic |
Antarctic Antarctic Peninsula Arthur Harbor Palmer Station Palmer-Station The Antarctic |
geographic_facet |
Antarctic Antarctic Peninsula Arthur Harbor Palmer Station Palmer-Station The Antarctic |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica Sea ice |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica Sea ice |
op_source |
Frontiers in Marine Science, Vol 8 (2021) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmars.2021.593566/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2021.593566 https://doaj.org/article/f47df1b6aa5240ff91047b19a9151d02 |
op_doi |
https://doi.org/10.3389/fmars.2021.593566 |
container_title |
Frontiers in Marine Science |
container_volume |
8 |
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1766272422209650688 |