Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis
In this study, five cyanobacteria strains ( Alkalinema aff. pantanalense LEGE15481, Cyanobium gracile LEGE12431, Nodosilinea ( Leptolyngbya ) antarctica LEGE13457, Cuspidothrix issatschenkoi LEGE03282 and Leptolyngbya -like sp. LEGE13412) from the Blue Biotechnology and Ecotoxicology Culture Collect...
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ftdoajarticles:oai:doaj.org/article:f4747a7e2d5f4112a40ccaeccef83370 2023-05-15T13:57:23+02:00 Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis Graciliana Lopes Duarte Clarinha Vitor Vasconcelos 2020-02-01T00:00:00Z https://doi.org/10.3390/microorganisms8020302 https://doaj.org/article/f4747a7e2d5f4112a40ccaeccef83370 EN eng MDPI AG https://www.mdpi.com/2076-2607/8/2/302 https://doaj.org/toc/2076-2607 2076-2607 doi:10.3390/microorganisms8020302 https://doaj.org/article/f4747a7e2d5f4112a40ccaeccef83370 Microorganisms, Vol 8, Iss 2, p 302 (2020) carotenoids cyanobacteria inflammation keratinocytes oxidative stress psoriasis Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.3390/microorganisms8020302 2022-12-31T04:06:53Z In this study, five cyanobacteria strains ( Alkalinema aff. pantanalense LEGE15481, Cyanobium gracile LEGE12431, Nodosilinea ( Leptolyngbya ) antarctica LEGE13457, Cuspidothrix issatschenkoi LEGE03282 and Leptolyngbya -like sp. LEGE13412) from the Blue Biotechnology and Ecotoxicology Culture Collection (LEGE CC) of CIIMAR were explored for their biotechnological potential in the treatment of psoriasis. Different extracts were characterized for their pigment profile by HPLC-PDA. The antioxidant potential of the extracts was assessed against the superoxide anion radical (O 2 •- ). Their anti-inflammatory and antiproliferative potential was assessed in vitro using the macrophages RAW 264.7 and the human keratinocytes HaCaT as cell-line models, respectively. Terrestrial and freshwater strains presented the highest carotenoid content (33193−63926 μg/g dry extract), with all- trans-β -carotene, zeaxanthin, echinenone and lutein derivatives being the most abundant carotenoids. Acetone was the most effective solvent for pigment extraction. The acetone extracts presented the lowest IC 50 values (0.29−0.38 mg dry extract/mL) regarding O 2 •- scavenging, and revealed anti-inflammatory potential, with N. antarctica LEGE13457, A. pantanalense LEGE15481 and Leptolyngbya -like sp. LEGE13412 reducing the nitric oxide (NO) in RAW 264.7 cell culture medium in about 25% ( p < 0.05). With the exception of A. pantanalense LEGE15481, all the extracts significantly reduced keratinocyte proliferation ( p < 0.05), demonstrating a selective toxicity among the different cell lines. Overall, Leptolyngbya -like sp. LEGE13412 and N. antarctica LEGE13457 seem promising for further exploitation in the framework of psoriasis, due to their antioxidant, anti-inflammatory and antiproliferative potential. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Microorganisms 8 2 302 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
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topic |
carotenoids cyanobacteria inflammation keratinocytes oxidative stress psoriasis Biology (General) QH301-705.5 |
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carotenoids cyanobacteria inflammation keratinocytes oxidative stress psoriasis Biology (General) QH301-705.5 Graciliana Lopes Duarte Clarinha Vitor Vasconcelos Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis |
topic_facet |
carotenoids cyanobacteria inflammation keratinocytes oxidative stress psoriasis Biology (General) QH301-705.5 |
description |
In this study, five cyanobacteria strains ( Alkalinema aff. pantanalense LEGE15481, Cyanobium gracile LEGE12431, Nodosilinea ( Leptolyngbya ) antarctica LEGE13457, Cuspidothrix issatschenkoi LEGE03282 and Leptolyngbya -like sp. LEGE13412) from the Blue Biotechnology and Ecotoxicology Culture Collection (LEGE CC) of CIIMAR were explored for their biotechnological potential in the treatment of psoriasis. Different extracts were characterized for their pigment profile by HPLC-PDA. The antioxidant potential of the extracts was assessed against the superoxide anion radical (O 2 •- ). Their anti-inflammatory and antiproliferative potential was assessed in vitro using the macrophages RAW 264.7 and the human keratinocytes HaCaT as cell-line models, respectively. Terrestrial and freshwater strains presented the highest carotenoid content (33193−63926 μg/g dry extract), with all- trans-β -carotene, zeaxanthin, echinenone and lutein derivatives being the most abundant carotenoids. Acetone was the most effective solvent for pigment extraction. The acetone extracts presented the lowest IC 50 values (0.29−0.38 mg dry extract/mL) regarding O 2 •- scavenging, and revealed anti-inflammatory potential, with N. antarctica LEGE13457, A. pantanalense LEGE15481 and Leptolyngbya -like sp. LEGE13412 reducing the nitric oxide (NO) in RAW 264.7 cell culture medium in about 25% ( p < 0.05). With the exception of A. pantanalense LEGE15481, all the extracts significantly reduced keratinocyte proliferation ( p < 0.05), demonstrating a selective toxicity among the different cell lines. Overall, Leptolyngbya -like sp. LEGE13412 and N. antarctica LEGE13457 seem promising for further exploitation in the framework of psoriasis, due to their antioxidant, anti-inflammatory and antiproliferative potential. |
format |
Article in Journal/Newspaper |
author |
Graciliana Lopes Duarte Clarinha Vitor Vasconcelos |
author_facet |
Graciliana Lopes Duarte Clarinha Vitor Vasconcelos |
author_sort |
Graciliana Lopes |
title |
Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis |
title_short |
Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis |
title_full |
Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis |
title_fullStr |
Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis |
title_full_unstemmed |
Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis |
title_sort |
carotenoids from cyanobacteria: a biotechnological approach for the topical treatment of psoriasis |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/microorganisms8020302 https://doaj.org/article/f4747a7e2d5f4112a40ccaeccef83370 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Microorganisms, Vol 8, Iss 2, p 302 (2020) |
op_relation |
https://www.mdpi.com/2076-2607/8/2/302 https://doaj.org/toc/2076-2607 2076-2607 doi:10.3390/microorganisms8020302 https://doaj.org/article/f4747a7e2d5f4112a40ccaeccef83370 |
op_doi |
https://doi.org/10.3390/microorganisms8020302 |
container_title |
Microorganisms |
container_volume |
8 |
container_issue |
2 |
container_start_page |
302 |
_version_ |
1766265064856223744 |