The Microwave-assisted Synthesis of Polyethersulfone (PES) as A Matrix in Immobilization of Candida antarctica Lipase B (Cal-B)
Candida antarctica lipase B (Cal-B) has been widely used in the hydrolysis reaction. However, it has some weaknesses, such as: forming of the heavy emulsion during the process, which is difficult to resolve and has no reusability. Therefore, it needs to be immobilized into a suitable matrix. One of...
Published in: | Bulletin of Chemical Reaction Engineering & Catalysis |
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Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS)
2017
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Online Access: | https://doi.org/10.9767/bcrec.12.3.774.343-350 https://doaj.org/article/f4687944c58d46709ae850601d3cdeeb |
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ftdoajarticles:oai:doaj.org/article:f4687944c58d46709ae850601d3cdeeb 2023-10-09T21:46:04+02:00 The Microwave-assisted Synthesis of Polyethersulfone (PES) as A Matrix in Immobilization of Candida antarctica Lipase B (Cal-B) Khusna Widhyahrini Nurrahmi Handayani Deana Wahyuningrum Santi Nurbaiti Cynthia Linaya Radiman 2017-12-01T00:00:00Z https://doi.org/10.9767/bcrec.12.3.774.343-350 https://doaj.org/article/f4687944c58d46709ae850601d3cdeeb EN ID eng ind Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS) https://journal.bcrec.id/index.php/bcrec/article/view/774 https://doaj.org/toc/1978-2993 1978-2993 doi:10.9767/bcrec.12.3.774.343-350 https://doaj.org/article/f4687944c58d46709ae850601d3cdeeb Bulletin of Chemical Reaction Engineering & Catalysis, Vol 12, Iss 3, Pp 343-350 (2017) polyethersulfone (pes) microwave assisted organic synthesis (maos) immobilization lipase candida antarctica lipase b (cal-b) Chemical engineering TP155-156 article 2017 ftdoajarticles https://doi.org/10.9767/bcrec.12.3.774.343-350 2023-09-24T00:35:30Z Candida antarctica lipase B (Cal-B) has been widely used in the hydrolysis reaction. However, it has some weaknesses, such as: forming of the heavy emulsion during the process, which is difficult to resolve and has no reusability. Therefore, it needs to be immobilized into a suitable matrix. One of the suitable supporting materials is polyethersulfone (PES) and its synthesis becames the objective of this paper. The PES was synthesized via a polycondensation reaction between hydroquinone and 4,4'-dichlorodiphenylsulfonein N-methylpyrrolidone (NMP) as solvent using Microwave Assisted Organic Synthesis (MAOS) method at170 °C for 66 minutes using an irradiation power of 300 watt. The synthesized PES was characterized by FTIR and 1H-NMR (500 MHz, DMSO-d6). Then the PES membrane was prepared from 20 % of the optimized mixtures of PES, PSf (polysulfone), and PEG (polyethylene glycol) dissolved in 80 % NMP. The Cal-B was immobilized on the PES membrane by mixing it in a shaker at 30 °C and 100 rpm for 24 h using phosphate buffered saline (PBS). The identification of the immobilized Cal-B was done by using FTIR-ATR spectroscopy and SEM micrographs. The results of Lowry assay showed that the ‘Cal-B immobilized’ blended-membrane has a loading capacity of 91 mg/cm2 in a membrane surface area of 17.34 cm2. In this work, the activity of immobilized Cal-B was twice higher than the native enzyme in p-NP (p-Nitrophenolpalmitate) hydrolyzing. The results indicated that the synthesized PES showed a good performance when used as a matrix in the immobilization of Cal-B. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Lowry ENVELOPE(-64.150,-64.150,-84.550,-84.550) Bulletin of Chemical Reaction Engineering & Catalysis 12 3 343 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Indonesian |
topic |
polyethersulfone (pes) microwave assisted organic synthesis (maos) immobilization lipase candida antarctica lipase b (cal-b) Chemical engineering TP155-156 |
spellingShingle |
polyethersulfone (pes) microwave assisted organic synthesis (maos) immobilization lipase candida antarctica lipase b (cal-b) Chemical engineering TP155-156 Khusna Widhyahrini Nurrahmi Handayani Deana Wahyuningrum Santi Nurbaiti Cynthia Linaya Radiman The Microwave-assisted Synthesis of Polyethersulfone (PES) as A Matrix in Immobilization of Candida antarctica Lipase B (Cal-B) |
topic_facet |
polyethersulfone (pes) microwave assisted organic synthesis (maos) immobilization lipase candida antarctica lipase b (cal-b) Chemical engineering TP155-156 |
description |
Candida antarctica lipase B (Cal-B) has been widely used in the hydrolysis reaction. However, it has some weaknesses, such as: forming of the heavy emulsion during the process, which is difficult to resolve and has no reusability. Therefore, it needs to be immobilized into a suitable matrix. One of the suitable supporting materials is polyethersulfone (PES) and its synthesis becames the objective of this paper. The PES was synthesized via a polycondensation reaction between hydroquinone and 4,4'-dichlorodiphenylsulfonein N-methylpyrrolidone (NMP) as solvent using Microwave Assisted Organic Synthesis (MAOS) method at170 °C for 66 minutes using an irradiation power of 300 watt. The synthesized PES was characterized by FTIR and 1H-NMR (500 MHz, DMSO-d6). Then the PES membrane was prepared from 20 % of the optimized mixtures of PES, PSf (polysulfone), and PEG (polyethylene glycol) dissolved in 80 % NMP. The Cal-B was immobilized on the PES membrane by mixing it in a shaker at 30 °C and 100 rpm for 24 h using phosphate buffered saline (PBS). The identification of the immobilized Cal-B was done by using FTIR-ATR spectroscopy and SEM micrographs. The results of Lowry assay showed that the ‘Cal-B immobilized’ blended-membrane has a loading capacity of 91 mg/cm2 in a membrane surface area of 17.34 cm2. In this work, the activity of immobilized Cal-B was twice higher than the native enzyme in p-NP (p-Nitrophenolpalmitate) hydrolyzing. The results indicated that the synthesized PES showed a good performance when used as a matrix in the immobilization of Cal-B. |
format |
Article in Journal/Newspaper |
author |
Khusna Widhyahrini Nurrahmi Handayani Deana Wahyuningrum Santi Nurbaiti Cynthia Linaya Radiman |
author_facet |
Khusna Widhyahrini Nurrahmi Handayani Deana Wahyuningrum Santi Nurbaiti Cynthia Linaya Radiman |
author_sort |
Khusna Widhyahrini |
title |
The Microwave-assisted Synthesis of Polyethersulfone (PES) as A Matrix in Immobilization of Candida antarctica Lipase B (Cal-B) |
title_short |
The Microwave-assisted Synthesis of Polyethersulfone (PES) as A Matrix in Immobilization of Candida antarctica Lipase B (Cal-B) |
title_full |
The Microwave-assisted Synthesis of Polyethersulfone (PES) as A Matrix in Immobilization of Candida antarctica Lipase B (Cal-B) |
title_fullStr |
The Microwave-assisted Synthesis of Polyethersulfone (PES) as A Matrix in Immobilization of Candida antarctica Lipase B (Cal-B) |
title_full_unstemmed |
The Microwave-assisted Synthesis of Polyethersulfone (PES) as A Matrix in Immobilization of Candida antarctica Lipase B (Cal-B) |
title_sort |
microwave-assisted synthesis of polyethersulfone (pes) as a matrix in immobilization of candida antarctica lipase b (cal-b) |
publisher |
Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS) |
publishDate |
2017 |
url |
https://doi.org/10.9767/bcrec.12.3.774.343-350 https://doaj.org/article/f4687944c58d46709ae850601d3cdeeb |
long_lat |
ENVELOPE(-64.150,-64.150,-84.550,-84.550) |
geographic |
Lowry |
geographic_facet |
Lowry |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Bulletin of Chemical Reaction Engineering & Catalysis, Vol 12, Iss 3, Pp 343-350 (2017) |
op_relation |
https://journal.bcrec.id/index.php/bcrec/article/view/774 https://doaj.org/toc/1978-2993 1978-2993 doi:10.9767/bcrec.12.3.774.343-350 https://doaj.org/article/f4687944c58d46709ae850601d3cdeeb |
op_doi |
https://doi.org/10.9767/bcrec.12.3.774.343-350 |
container_title |
Bulletin of Chemical Reaction Engineering & Catalysis |
container_volume |
12 |
container_issue |
3 |
container_start_page |
343 |
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1779321726257070080 |