A novel procedure for precise quantification of Schistosoma japonicum eggs in bovine feces.
Schistosomiasis japonica is a zoonosis with a number of mammalian species acting as reservoir hosts, including water buffaloes which can contribute up to 75% to human transmission in the People's Republic of China. Determining prevalence and intensity of Schistosoma japonicum in mammalian hosts...
Published in: | PLoS Neglected Tropical Diseases |
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Online Access: | https://doi.org/10.1371/journal.pntd.0001885 https://doaj.org/article/f4573e85e2984a889b7f9f8318adf60d |
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ftdoajarticles:oai:doaj.org/article:f4573e85e2984a889b7f9f8318adf60d 2023-05-15T15:10:40+02:00 A novel procedure for precise quantification of Schistosoma japonicum eggs in bovine feces. Bin Xu Catherine A Gordon Wei Hu Donald P McManus Hong-Gen Chen Darren J Gray Chuan Ju Xiao-Jun Zeng Geoffrey N Gobert Jun Ge Wei-Ming Lan Shu-Ying Xie Wei-Sheng Jiang Allen G Ross Luz P Acosta Remigio Olveda Zheng Feng 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001885 https://doaj.org/article/f4573e85e2984a889b7f9f8318adf60d EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3499414?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001885 https://doaj.org/article/f4573e85e2984a889b7f9f8318adf60d PLoS Neglected Tropical Diseases, Vol 6, Iss 11, p e1885 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001885 2022-12-31T12:57:40Z Schistosomiasis japonica is a zoonosis with a number of mammalian species acting as reservoir hosts, including water buffaloes which can contribute up to 75% to human transmission in the People's Republic of China. Determining prevalence and intensity of Schistosoma japonicum in mammalian hosts is important for calculating transmission rates and determining environmental contamination. A new procedure, the formalin-ethyl acetate sedimentation-digestion (FEA-SD) technique, for increased visualization of S. japonicum eggs in bovine feces, is described that is an effective technique for identifying and quantifying S. japonicum eggs in fecal samples from naturally infected Chinese water buffaloes and from carabao (water buffalo) in the Philippines. The procedure involves filtration, sedimentation, potassium hydroxide digestion and centrifugation steps prior to microscopy. Bulk debris, including the dense cellulosic material present in bovine feces, often obscures schistosome eggs with the result that prevalence and infection intensity based on direct visualization cannot be made accurately. This technique removes nearly 70% of debris from the fecal samples and renders the remaining debris translucent. It allows improved microscopic visualization of S. japonicum eggs and provides an accurate quantitative method for the estimation of infection in bovines and other ruminant reservoir hosts. We show that the FEA-SD technique could be of considerable value if applied as a surveillance tool for animal reservoirs of S. japonicum, particularly in areas with low to high infection intensity, or where, following control efforts, there is suspected elimination of schistosomiasis japonica. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 6 11 e1885 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Bin Xu Catherine A Gordon Wei Hu Donald P McManus Hong-Gen Chen Darren J Gray Chuan Ju Xiao-Jun Zeng Geoffrey N Gobert Jun Ge Wei-Ming Lan Shu-Ying Xie Wei-Sheng Jiang Allen G Ross Luz P Acosta Remigio Olveda Zheng Feng A novel procedure for precise quantification of Schistosoma japonicum eggs in bovine feces. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Schistosomiasis japonica is a zoonosis with a number of mammalian species acting as reservoir hosts, including water buffaloes which can contribute up to 75% to human transmission in the People's Republic of China. Determining prevalence and intensity of Schistosoma japonicum in mammalian hosts is important for calculating transmission rates and determining environmental contamination. A new procedure, the formalin-ethyl acetate sedimentation-digestion (FEA-SD) technique, for increased visualization of S. japonicum eggs in bovine feces, is described that is an effective technique for identifying and quantifying S. japonicum eggs in fecal samples from naturally infected Chinese water buffaloes and from carabao (water buffalo) in the Philippines. The procedure involves filtration, sedimentation, potassium hydroxide digestion and centrifugation steps prior to microscopy. Bulk debris, including the dense cellulosic material present in bovine feces, often obscures schistosome eggs with the result that prevalence and infection intensity based on direct visualization cannot be made accurately. This technique removes nearly 70% of debris from the fecal samples and renders the remaining debris translucent. It allows improved microscopic visualization of S. japonicum eggs and provides an accurate quantitative method for the estimation of infection in bovines and other ruminant reservoir hosts. We show that the FEA-SD technique could be of considerable value if applied as a surveillance tool for animal reservoirs of S. japonicum, particularly in areas with low to high infection intensity, or where, following control efforts, there is suspected elimination of schistosomiasis japonica. |
format |
Article in Journal/Newspaper |
author |
Bin Xu Catherine A Gordon Wei Hu Donald P McManus Hong-Gen Chen Darren J Gray Chuan Ju Xiao-Jun Zeng Geoffrey N Gobert Jun Ge Wei-Ming Lan Shu-Ying Xie Wei-Sheng Jiang Allen G Ross Luz P Acosta Remigio Olveda Zheng Feng |
author_facet |
Bin Xu Catherine A Gordon Wei Hu Donald P McManus Hong-Gen Chen Darren J Gray Chuan Ju Xiao-Jun Zeng Geoffrey N Gobert Jun Ge Wei-Ming Lan Shu-Ying Xie Wei-Sheng Jiang Allen G Ross Luz P Acosta Remigio Olveda Zheng Feng |
author_sort |
Bin Xu |
title |
A novel procedure for precise quantification of Schistosoma japonicum eggs in bovine feces. |
title_short |
A novel procedure for precise quantification of Schistosoma japonicum eggs in bovine feces. |
title_full |
A novel procedure for precise quantification of Schistosoma japonicum eggs in bovine feces. |
title_fullStr |
A novel procedure for precise quantification of Schistosoma japonicum eggs in bovine feces. |
title_full_unstemmed |
A novel procedure for precise quantification of Schistosoma japonicum eggs in bovine feces. |
title_sort |
novel procedure for precise quantification of schistosoma japonicum eggs in bovine feces. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001885 https://doaj.org/article/f4573e85e2984a889b7f9f8318adf60d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 11, p e1885 (2012) |
op_relation |
http://europepmc.org/articles/PMC3499414?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001885 https://doaj.org/article/f4573e85e2984a889b7f9f8318adf60d |
op_doi |
https://doi.org/10.1371/journal.pntd.0001885 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
6 |
container_issue |
11 |
container_start_page |
e1885 |
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1766341653262499840 |