Study on the Extraction Technology of Candida antarctica Lipase B by Foam Separation
Candida antarctica Lipase B (CALB) has a wide range of applications in many fields. In this study, Pichia pastoris was used to express CALB for fermentation tank culture. Sodium dodecyl sulfate (SDS) was used as a surfactant, and foam separation technology was used to explore the best experimental c...
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ftdoajarticles:oai:doaj.org/article:f4560239d80647b4be02c19baadafe3d 2023-05-15T13:45:15+02:00 Study on the Extraction Technology of Candida antarctica Lipase B by Foam Separation Wenyao Shao Ying Lin Yinghua Lu 2020-12-01T00:00:00Z https://doi.org/10.3390/pr9010014 https://doaj.org/article/f4560239d80647b4be02c19baadafe3d EN eng MDPI AG https://www.mdpi.com/2227-9717/9/1/14 https://doaj.org/toc/2227-9717 doi:10.3390/pr9010014 2227-9717 https://doaj.org/article/f4560239d80647b4be02c19baadafe3d Processes, Vol 9, Iss 14, p 14 (2020) foam separation fermentation lipase surfactant enrichment ratio recovery rate Chemical technology TP1-1185 Chemistry QD1-999 article 2020 ftdoajarticles https://doi.org/10.3390/pr9010014 2022-12-30T22:03:30Z Candida antarctica Lipase B (CALB) has a wide range of applications in many fields. In this study, Pichia pastoris was used to express CALB for fermentation tank culture. Sodium dodecyl sulfate (SDS) was used as a surfactant, and foam separation technology was used to explore the best experimental conditions for the harvest of CALB. The results showed that the optimal technological conditions for the foam separation and recovery of CALB were as follows: liquid volume was 150 mL, separating gas velocity was 600 mL/min, pH value was 7, and surfactant SDS concentration was 0.5 mg/mL. Under these conditions, the enrichment ratio of CALB was 0.95, and recovery rate R was 80.32%, respectively, indicating that the foam separation technology is feasible to extract lipase B. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Processes 9 1 14 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
foam separation fermentation lipase surfactant enrichment ratio recovery rate Chemical technology TP1-1185 Chemistry QD1-999 |
spellingShingle |
foam separation fermentation lipase surfactant enrichment ratio recovery rate Chemical technology TP1-1185 Chemistry QD1-999 Wenyao Shao Ying Lin Yinghua Lu Study on the Extraction Technology of Candida antarctica Lipase B by Foam Separation |
topic_facet |
foam separation fermentation lipase surfactant enrichment ratio recovery rate Chemical technology TP1-1185 Chemistry QD1-999 |
description |
Candida antarctica Lipase B (CALB) has a wide range of applications in many fields. In this study, Pichia pastoris was used to express CALB for fermentation tank culture. Sodium dodecyl sulfate (SDS) was used as a surfactant, and foam separation technology was used to explore the best experimental conditions for the harvest of CALB. The results showed that the optimal technological conditions for the foam separation and recovery of CALB were as follows: liquid volume was 150 mL, separating gas velocity was 600 mL/min, pH value was 7, and surfactant SDS concentration was 0.5 mg/mL. Under these conditions, the enrichment ratio of CALB was 0.95, and recovery rate R was 80.32%, respectively, indicating that the foam separation technology is feasible to extract lipase B. |
format |
Article in Journal/Newspaper |
author |
Wenyao Shao Ying Lin Yinghua Lu |
author_facet |
Wenyao Shao Ying Lin Yinghua Lu |
author_sort |
Wenyao Shao |
title |
Study on the Extraction Technology of Candida antarctica Lipase B by Foam Separation |
title_short |
Study on the Extraction Technology of Candida antarctica Lipase B by Foam Separation |
title_full |
Study on the Extraction Technology of Candida antarctica Lipase B by Foam Separation |
title_fullStr |
Study on the Extraction Technology of Candida antarctica Lipase B by Foam Separation |
title_full_unstemmed |
Study on the Extraction Technology of Candida antarctica Lipase B by Foam Separation |
title_sort |
study on the extraction technology of candida antarctica lipase b by foam separation |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/pr9010014 https://doaj.org/article/f4560239d80647b4be02c19baadafe3d |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Processes, Vol 9, Iss 14, p 14 (2020) |
op_relation |
https://www.mdpi.com/2227-9717/9/1/14 https://doaj.org/toc/2227-9717 doi:10.3390/pr9010014 2227-9717 https://doaj.org/article/f4560239d80647b4be02c19baadafe3d |
op_doi |
https://doi.org/10.3390/pr9010014 |
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