Pyriproxyfen treated surface exposure exhibits reproductive disruption in dengue vector Aedes aegypti.
Background Reduced susceptibility of mosquito vectors to currently used insecticides hampers control interventions. Recently pyriproxyfen, an insect growth regulator has been demonstrated to effectively reduce the reproductive potential in vector mosquitoes. Methods Pyriproxyfen (PPF), in different...
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ftdoajarticles:oai:doaj.org/article:f44e95788cc14357a36b7fde7a6cad0d 2023-05-15T15:16:18+02:00 Pyriproxyfen treated surface exposure exhibits reproductive disruption in dengue vector Aedes aegypti. Kavita Yadav Sunil Dhiman B N Acharya Rama Rao Ghorpade Devanathan Sukumaran 2019-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007842 https://doaj.org/article/f44e95788cc14357a36b7fde7a6cad0d EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007842 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007842 https://doaj.org/article/f44e95788cc14357a36b7fde7a6cad0d PLoS Neglected Tropical Diseases, Vol 13, Iss 11, p e0007842 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007842 2022-12-31T07:48:33Z Background Reduced susceptibility of mosquito vectors to currently used insecticides hampers control interventions. Recently pyriproxyfen, an insect growth regulator has been demonstrated to effectively reduce the reproductive potential in vector mosquitoes. Methods Pyriproxyfen (PPF), in different concentrations (0.75%, 0.075%, 0.0075%, and 0.00075%) was applied on papers and Indian wild type Aedes aegypti female mosquitoes (N ≥ 20 for each treatment) were exposed onto it as per WHO guidelines, to study the reproductive disruption. PPF concentration on treated papers was quantitatively cross-determined using HPLC method. Reduction in fecundity, fertility and adult emergence in exposed female Ae. aegypti was determined. Abnormal development in ovary and eggs of exposed females was studied microscopically after different time intervals. Results Eggs laid, eggs hatched, pupae formed and adults emerged per female exposed in both before blood meal and after blood meal groups declined significantly from lowest to highest concentration of PPF (F ≥ 5.2; p < 0.02). Adult emergence inhibition in females exposed to PPF before and after blood meal groups ranged from 58.8% [OR = 0.18 (95% CI = 0.09-0.36)] to 79.2% [OR = 0.04 (95% CI = 0.02-0.10)] and 64.4% [OR = 0.12 (95% CI = 0.05-0.28)] to 77.1% [OR = 0.05 (95% CI = 0.02-0.14)] respectively in different concentrations. The probit model used suggested that FI50 (50% fertility inhibition) and EI50 (50% emergence inhibition) were 0.002% (p = 0.82) and 0.0001% (p = 0.99) for females exposed before blood meal, while 0.01% (p = 0.63) and <0.0001% (p = 0.98) for the females exposed after blood meal, respectively. The eggs laid by the females exposed to PPF-treated surface showed altered body organization, desegmentation and disoriented abdominal and cervical regions in the developing embryo. Quantification of PPF on impregnated papers showed that it was uniformly distributed throughout the matrix. Conclusions The present study has shown that tarsal contact to PPF-treated ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Indian PLOS Neglected Tropical Diseases 13 11 e0007842 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Kavita Yadav Sunil Dhiman B N Acharya Rama Rao Ghorpade Devanathan Sukumaran Pyriproxyfen treated surface exposure exhibits reproductive disruption in dengue vector Aedes aegypti. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Reduced susceptibility of mosquito vectors to currently used insecticides hampers control interventions. Recently pyriproxyfen, an insect growth regulator has been demonstrated to effectively reduce the reproductive potential in vector mosquitoes. Methods Pyriproxyfen (PPF), in different concentrations (0.75%, 0.075%, 0.0075%, and 0.00075%) was applied on papers and Indian wild type Aedes aegypti female mosquitoes (N ≥ 20 for each treatment) were exposed onto it as per WHO guidelines, to study the reproductive disruption. PPF concentration on treated papers was quantitatively cross-determined using HPLC method. Reduction in fecundity, fertility and adult emergence in exposed female Ae. aegypti was determined. Abnormal development in ovary and eggs of exposed females was studied microscopically after different time intervals. Results Eggs laid, eggs hatched, pupae formed and adults emerged per female exposed in both before blood meal and after blood meal groups declined significantly from lowest to highest concentration of PPF (F ≥ 5.2; p < 0.02). Adult emergence inhibition in females exposed to PPF before and after blood meal groups ranged from 58.8% [OR = 0.18 (95% CI = 0.09-0.36)] to 79.2% [OR = 0.04 (95% CI = 0.02-0.10)] and 64.4% [OR = 0.12 (95% CI = 0.05-0.28)] to 77.1% [OR = 0.05 (95% CI = 0.02-0.14)] respectively in different concentrations. The probit model used suggested that FI50 (50% fertility inhibition) and EI50 (50% emergence inhibition) were 0.002% (p = 0.82) and 0.0001% (p = 0.99) for females exposed before blood meal, while 0.01% (p = 0.63) and <0.0001% (p = 0.98) for the females exposed after blood meal, respectively. The eggs laid by the females exposed to PPF-treated surface showed altered body organization, desegmentation and disoriented abdominal and cervical regions in the developing embryo. Quantification of PPF on impregnated papers showed that it was uniformly distributed throughout the matrix. Conclusions The present study has shown that tarsal contact to PPF-treated ... |
format |
Article in Journal/Newspaper |
author |
Kavita Yadav Sunil Dhiman B N Acharya Rama Rao Ghorpade Devanathan Sukumaran |
author_facet |
Kavita Yadav Sunil Dhiman B N Acharya Rama Rao Ghorpade Devanathan Sukumaran |
author_sort |
Kavita Yadav |
title |
Pyriproxyfen treated surface exposure exhibits reproductive disruption in dengue vector Aedes aegypti. |
title_short |
Pyriproxyfen treated surface exposure exhibits reproductive disruption in dengue vector Aedes aegypti. |
title_full |
Pyriproxyfen treated surface exposure exhibits reproductive disruption in dengue vector Aedes aegypti. |
title_fullStr |
Pyriproxyfen treated surface exposure exhibits reproductive disruption in dengue vector Aedes aegypti. |
title_full_unstemmed |
Pyriproxyfen treated surface exposure exhibits reproductive disruption in dengue vector Aedes aegypti. |
title_sort |
pyriproxyfen treated surface exposure exhibits reproductive disruption in dengue vector aedes aegypti. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007842 https://doaj.org/article/f44e95788cc14357a36b7fde7a6cad0d |
geographic |
Arctic Indian |
geographic_facet |
Arctic Indian |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 11, p e0007842 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007842 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007842 https://doaj.org/article/f44e95788cc14357a36b7fde7a6cad0d |
op_doi |
https://doi.org/10.1371/journal.pntd.0007842 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
11 |
container_start_page |
e0007842 |
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1766346593197359104 |