Antibacterial properties of lucifensin in Lucilia sericata maggots after septic injury
Objective: To investigate the antibacterial properties of lucifensin in maggots of Lucilia sericata after septic injury. Methods: In our preliminary study we have shown that injuring the maggots with a needle soaked in lipopolysaccharide solution induced within 24 h lucifensin expression in the fat...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2014
|
Subjects: | |
Online Access: | https://doi.org/10.12980/APJTB.4.2014C1134 https://doaj.org/article/f420147309084eed9f54e580fb0e37e6 |
id |
ftdoajarticles:oai:doaj.org/article:f420147309084eed9f54e580fb0e37e6 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:f420147309084eed9f54e580fb0e37e6 2023-05-15T15:05:07+02:00 Antibacterial properties of lucifensin in Lucilia sericata maggots after septic injury Ivana Valachova Emanuel Prochazka Jana Bohova Petr Novak Peter Takac Juraj Majtan 2014-05-01T00:00:00Z https://doi.org/10.12980/APJTB.4.2014C1134 https://doaj.org/article/f420147309084eed9f54e580fb0e37e6 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115303269 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C1134 https://doaj.org/article/f420147309084eed9f54e580fb0e37e6 Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 5, Pp 358-361 (2014) Lucilia sericata Wound bacteria Defensin Lucifensin Immune-challenge Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.12980/APJTB.4.2014C1134 2022-12-30T22:33:39Z Objective: To investigate the antibacterial properties of lucifensin in maggots of Lucilia sericata after septic injury. Methods: In our preliminary study we have shown that injuring the maggots with a needle soaked in lipopolysaccharide solution induced within 24 h lucifensin expression in the fat body and in the grease coupler of the salivary glands. It is assumed that lucifensin is secreted solely from this tissue into the haemolymph (similar to other insect defensins) and not into secreted/excreted products. We used highâperformance liquid chromatography fractionation and radial diffusion assay to investigate the antibacterial properties of haemolymph extracted from larvae after septic injury. Results: After septic injury, production of lucifensin in the haemolymph is increased. This led to higher antibacterial activity of such haemolymph in comparison to non-stimulated larvae. Coclusions: These results suggest that beside the previously demonstrated role of lucifensin in the debridement therapy, lucifensin is simultaneously important as a part of the systematic immune response. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 5 358 361 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Lucilia sericata Wound bacteria Defensin Lucifensin Immune-challenge Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Lucilia sericata Wound bacteria Defensin Lucifensin Immune-challenge Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Ivana Valachova Emanuel Prochazka Jana Bohova Petr Novak Peter Takac Juraj Majtan Antibacterial properties of lucifensin in Lucilia sericata maggots after septic injury |
topic_facet |
Lucilia sericata Wound bacteria Defensin Lucifensin Immune-challenge Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the antibacterial properties of lucifensin in maggots of Lucilia sericata after septic injury. Methods: In our preliminary study we have shown that injuring the maggots with a needle soaked in lipopolysaccharide solution induced within 24 h lucifensin expression in the fat body and in the grease coupler of the salivary glands. It is assumed that lucifensin is secreted solely from this tissue into the haemolymph (similar to other insect defensins) and not into secreted/excreted products. We used highâperformance liquid chromatography fractionation and radial diffusion assay to investigate the antibacterial properties of haemolymph extracted from larvae after septic injury. Results: After septic injury, production of lucifensin in the haemolymph is increased. This led to higher antibacterial activity of such haemolymph in comparison to non-stimulated larvae. Coclusions: These results suggest that beside the previously demonstrated role of lucifensin in the debridement therapy, lucifensin is simultaneously important as a part of the systematic immune response. |
format |
Article in Journal/Newspaper |
author |
Ivana Valachova Emanuel Prochazka Jana Bohova Petr Novak Peter Takac Juraj Majtan |
author_facet |
Ivana Valachova Emanuel Prochazka Jana Bohova Petr Novak Peter Takac Juraj Majtan |
author_sort |
Ivana Valachova |
title |
Antibacterial properties of lucifensin in Lucilia sericata maggots after septic injury |
title_short |
Antibacterial properties of lucifensin in Lucilia sericata maggots after septic injury |
title_full |
Antibacterial properties of lucifensin in Lucilia sericata maggots after septic injury |
title_fullStr |
Antibacterial properties of lucifensin in Lucilia sericata maggots after septic injury |
title_full_unstemmed |
Antibacterial properties of lucifensin in Lucilia sericata maggots after septic injury |
title_sort |
antibacterial properties of lucifensin in lucilia sericata maggots after septic injury |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.12980/APJTB.4.2014C1134 https://doaj.org/article/f420147309084eed9f54e580fb0e37e6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 5, Pp 358-361 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115303269 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C1134 https://doaj.org/article/f420147309084eed9f54e580fb0e37e6 |
op_doi |
https://doi.org/10.12980/APJTB.4.2014C1134 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_issue |
5 |
container_start_page |
358 |
op_container_end_page |
361 |
_version_ |
1766336863333777408 |