Molecular detection of HPV 16 and 18 in cervical samples of patients from Belo Horizonte, Minas Gerais, Brazil Detecção molecular de HPV 16 e 18 em amostras cervicais de pacientes de Belo Horizonte, Minas Gerais, Brasil
PURPOSE: The aim of this study was to investigate the frequency of HPV infection and the types 16 and 18 in cervical samples from patients attended at two public health services of the city of Belo Horizonte, MG. METHODS: Cervical samples from 174 patients were collected for cytopathological and mol...
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ftdoajarticles:oai:doaj.org/article:f40ce6721d5c4ac1ae0facd7ddef9193 2024-09-09T19:28:39+00:00 Molecular detection of HPV 16 and 18 in cervical samples of patients from Belo Horizonte, Minas Gerais, Brazil Detecção molecular de HPV 16 e 18 em amostras cervicais de pacientes de Belo Horizonte, Minas Gerais, Brasil Taíse Palmeiras Freitas Bianca Bianco do Carmo Francisco Danilo Ferreira Paula Lucas Fonseca Rodrigues Ana Paula Fernandes Paula Ávila Fernandes 2007-10-01T00:00:00Z https://doi.org/10.1590/S0036-46652007000500005 https://doaj.org/article/f40ce6721d5c4ac1ae0facd7ddef9193 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652007000500005 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652007000500005 0036-4665 1678-9946 https://doaj.org/article/f40ce6721d5c4ac1ae0facd7ddef9193 Revista do Instituto de Medicina Tropical de São Paulo, Vol 49, Iss 5, Pp 297-301 (2007) HPV Cervical cancer PCR Genotyping Cytology Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2007 ftdoajarticles https://doi.org/10.1590/S0036-46652007000500005 2024-08-05T17:49:30Z PURPOSE: The aim of this study was to investigate the frequency of HPV infection and the types 16 and 18 in cervical samples from patients attended at two public health services of the city of Belo Horizonte, MG. METHODS: Cervical samples from 174 patients were collected for cytopathological and molecular tests. HPV infection was searched by PCR utilizing MY09 and MY11 primers or HPV 16 and HPV 18 specific primers. RESULTS: Amongst the 174 samples analyzed, 20.7% presented squamous intraepithelial and/or invasive lesions detected on cytopathological analysis and of those, 94.4% were infected by HPV. HPV 16 was found in 20% of the cases of low-grade squamous intraepithelial lesions and in 40% and 50% of high-grade squamous intraepithelial lesion and squamous invasive carcinoma, respectively. HPV 18 was detected in 6.7% of the low-grade lesion samples and in two HPV16 co-infected samples. In 50% of the cases of high-grade lesion, the HPV type was not determined. CONCLUSION: The HPV 16 was the virus type more frequently detected. However, more than 50% of the positive samples at the cytopathological analysis were negative for HPV 16 and 18, indicating that possibly other virus types are present in relative high frequencies in the studied population. OBJETIVOS: O objetivo deste estudo foi investigar a freqüência da infecção por HPV e dos tipos 16 e 18 em amostras cervicais de pacientes atendidas em dois serviços públicos da cidade de Belo Horizonte-MG. MÉTODOS: Amostras cervicais de 174 pacientes foram coletadas para estudo citopatológico e molecular. A pesquisa da infecção por HPV foi feita através da PCR utilizando os oligonucleotídeos MY09/MY11. Os tipos virais 16 e 18 foram pesquisados através da utilização de oligonucleotídeos específicos. RESULTADOS: Dentre as 174 amostras analisadas, 20,7% apresentaram lesões escamosas intra-epiteliais e/ou invasoras detectadas na análise citopatológica, das quais 94,4% mostraram infecção por HPV. O HPV 16 foi encontrado em torno de 20% dos casos de lesão escamosa ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 49 5 297 301 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
HPV Cervical cancer PCR Genotyping Cytology Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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HPV Cervical cancer PCR Genotyping Cytology Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Taíse Palmeiras Freitas Bianca Bianco do Carmo Francisco Danilo Ferreira Paula Lucas Fonseca Rodrigues Ana Paula Fernandes Paula Ávila Fernandes Molecular detection of HPV 16 and 18 in cervical samples of patients from Belo Horizonte, Minas Gerais, Brazil Detecção molecular de HPV 16 e 18 em amostras cervicais de pacientes de Belo Horizonte, Minas Gerais, Brasil |
topic_facet |
HPV Cervical cancer PCR Genotyping Cytology Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
PURPOSE: The aim of this study was to investigate the frequency of HPV infection and the types 16 and 18 in cervical samples from patients attended at two public health services of the city of Belo Horizonte, MG. METHODS: Cervical samples from 174 patients were collected for cytopathological and molecular tests. HPV infection was searched by PCR utilizing MY09 and MY11 primers or HPV 16 and HPV 18 specific primers. RESULTS: Amongst the 174 samples analyzed, 20.7% presented squamous intraepithelial and/or invasive lesions detected on cytopathological analysis and of those, 94.4% were infected by HPV. HPV 16 was found in 20% of the cases of low-grade squamous intraepithelial lesions and in 40% and 50% of high-grade squamous intraepithelial lesion and squamous invasive carcinoma, respectively. HPV 18 was detected in 6.7% of the low-grade lesion samples and in two HPV16 co-infected samples. In 50% of the cases of high-grade lesion, the HPV type was not determined. CONCLUSION: The HPV 16 was the virus type more frequently detected. However, more than 50% of the positive samples at the cytopathological analysis were negative for HPV 16 and 18, indicating that possibly other virus types are present in relative high frequencies in the studied population. OBJETIVOS: O objetivo deste estudo foi investigar a freqüência da infecção por HPV e dos tipos 16 e 18 em amostras cervicais de pacientes atendidas em dois serviços públicos da cidade de Belo Horizonte-MG. MÉTODOS: Amostras cervicais de 174 pacientes foram coletadas para estudo citopatológico e molecular. A pesquisa da infecção por HPV foi feita através da PCR utilizando os oligonucleotídeos MY09/MY11. Os tipos virais 16 e 18 foram pesquisados através da utilização de oligonucleotídeos específicos. RESULTADOS: Dentre as 174 amostras analisadas, 20,7% apresentaram lesões escamosas intra-epiteliais e/ou invasoras detectadas na análise citopatológica, das quais 94,4% mostraram infecção por HPV. O HPV 16 foi encontrado em torno de 20% dos casos de lesão escamosa ... |
format |
Article in Journal/Newspaper |
author |
Taíse Palmeiras Freitas Bianca Bianco do Carmo Francisco Danilo Ferreira Paula Lucas Fonseca Rodrigues Ana Paula Fernandes Paula Ávila Fernandes |
author_facet |
Taíse Palmeiras Freitas Bianca Bianco do Carmo Francisco Danilo Ferreira Paula Lucas Fonseca Rodrigues Ana Paula Fernandes Paula Ávila Fernandes |
author_sort |
Taíse Palmeiras Freitas |
title |
Molecular detection of HPV 16 and 18 in cervical samples of patients from Belo Horizonte, Minas Gerais, Brazil Detecção molecular de HPV 16 e 18 em amostras cervicais de pacientes de Belo Horizonte, Minas Gerais, Brasil |
title_short |
Molecular detection of HPV 16 and 18 in cervical samples of patients from Belo Horizonte, Minas Gerais, Brazil Detecção molecular de HPV 16 e 18 em amostras cervicais de pacientes de Belo Horizonte, Minas Gerais, Brasil |
title_full |
Molecular detection of HPV 16 and 18 in cervical samples of patients from Belo Horizonte, Minas Gerais, Brazil Detecção molecular de HPV 16 e 18 em amostras cervicais de pacientes de Belo Horizonte, Minas Gerais, Brasil |
title_fullStr |
Molecular detection of HPV 16 and 18 in cervical samples of patients from Belo Horizonte, Minas Gerais, Brazil Detecção molecular de HPV 16 e 18 em amostras cervicais de pacientes de Belo Horizonte, Minas Gerais, Brasil |
title_full_unstemmed |
Molecular detection of HPV 16 and 18 in cervical samples of patients from Belo Horizonte, Minas Gerais, Brazil Detecção molecular de HPV 16 e 18 em amostras cervicais de pacientes de Belo Horizonte, Minas Gerais, Brasil |
title_sort |
molecular detection of hpv 16 and 18 in cervical samples of patients from belo horizonte, minas gerais, brazil detecção molecular de hpv 16 e 18 em amostras cervicais de pacientes de belo horizonte, minas gerais, brasil |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2007 |
url |
https://doi.org/10.1590/S0036-46652007000500005 https://doaj.org/article/f40ce6721d5c4ac1ae0facd7ddef9193 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 49, Iss 5, Pp 297-301 (2007) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652007000500005 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652007000500005 0036-4665 1678-9946 https://doaj.org/article/f40ce6721d5c4ac1ae0facd7ddef9193 |
op_doi |
https://doi.org/10.1590/S0036-46652007000500005 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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49 |
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