Severe hemorrhagic fever in strain 13/N guinea pigs infected with Lujo virus.
Lujo virus (LUJV) is a novel member of the Arenaviridae family that was first identified in 2008 after an outbreak of severe hemorrhagic fever (HF). In what was a small but rapidly progressing outbreak, this previously unknown virus was transmitted from the critically ill index patient to 4 attendin...
Published in: | PLOS Neglected Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:f3f60ea17bdd4573b053e1114c5e9293 2023-05-15T15:11:50+02:00 Severe hemorrhagic fever in strain 13/N guinea pigs infected with Lujo virus. Brian H Bird Kimberly A Dodd Bobbie R Erickson César G Albariño Ayan K Chakrabarti Laura K McMullan Eric Bergeron Ute Ströeher Deborah Cannon Brock Martin JoAnn D Coleman-McCray Stuart T Nichol Christina F Spiropoulou 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001801 https://doaj.org/article/f3f60ea17bdd4573b053e1114c5e9293 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3429401?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 doi:10.1371/journal.pntd.0001801 1935-2727 1935-2735 https://doaj.org/article/f3f60ea17bdd4573b053e1114c5e9293 PLoS Neglected Tropical Diseases, Vol 6, Iss 8, p e1801 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001801 2022-12-31T02:04:12Z Lujo virus (LUJV) is a novel member of the Arenaviridae family that was first identified in 2008 after an outbreak of severe hemorrhagic fever (HF). In what was a small but rapidly progressing outbreak, this previously unknown virus was transmitted from the critically ill index patient to 4 attending healthcare workers. Four persons died during this outbreak, for a total case fatality of 80% (4/5). The suspected rodent source of the initial exposure to LUJV remains a mystery. Because of the ease of transmission, high case fatality, and novel nature of LUJV, we sought to establish an animal model of LUJV HF. Initial attempts in mice failed, but infection of inbred strain 13/N guinea pigs resulted in lethal disease. A total of 41 adult strain 13/N guinea pigs were infected with either wild-type LUJV or a full-length recombinant LUJV. Results demonstrated that strain 13/N guinea pigs provide an excellent model of severe and lethal LUJV HF that closely resembles what is known of the human disease. All infected animals experienced consistent weight loss (3-5% per day) and clinical illness characterized by ocular discharge, ruffled fur, hunched posture, and lethargy. Uniform lethality occurred by 11-16 days post-infection. All animals developed disseminated LUJV infection in various organs (liver, spleen, lung, and kidney), and leukopenia, lymphopenia, thrombocytopenia, coagulopathy, and elevated transaminase levels. Serial euthanasia studies revealed a temporal pattern of virus dissemination and increasing severity of disease, primarily targeting the liver, spleen, lungs, and lower gastrointestinal tract. Establishing an animal LUJV model is an important first step towards understanding the high pathogenicity of LUJV and developing vaccines and antiviral therapeutic drugs for this highly transmissible and lethal emerging pathogen. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 6 8 e1801 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Brian H Bird Kimberly A Dodd Bobbie R Erickson César G Albariño Ayan K Chakrabarti Laura K McMullan Eric Bergeron Ute Ströeher Deborah Cannon Brock Martin JoAnn D Coleman-McCray Stuart T Nichol Christina F Spiropoulou Severe hemorrhagic fever in strain 13/N guinea pigs infected with Lujo virus. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Lujo virus (LUJV) is a novel member of the Arenaviridae family that was first identified in 2008 after an outbreak of severe hemorrhagic fever (HF). In what was a small but rapidly progressing outbreak, this previously unknown virus was transmitted from the critically ill index patient to 4 attending healthcare workers. Four persons died during this outbreak, for a total case fatality of 80% (4/5). The suspected rodent source of the initial exposure to LUJV remains a mystery. Because of the ease of transmission, high case fatality, and novel nature of LUJV, we sought to establish an animal model of LUJV HF. Initial attempts in mice failed, but infection of inbred strain 13/N guinea pigs resulted in lethal disease. A total of 41 adult strain 13/N guinea pigs were infected with either wild-type LUJV or a full-length recombinant LUJV. Results demonstrated that strain 13/N guinea pigs provide an excellent model of severe and lethal LUJV HF that closely resembles what is known of the human disease. All infected animals experienced consistent weight loss (3-5% per day) and clinical illness characterized by ocular discharge, ruffled fur, hunched posture, and lethargy. Uniform lethality occurred by 11-16 days post-infection. All animals developed disseminated LUJV infection in various organs (liver, spleen, lung, and kidney), and leukopenia, lymphopenia, thrombocytopenia, coagulopathy, and elevated transaminase levels. Serial euthanasia studies revealed a temporal pattern of virus dissemination and increasing severity of disease, primarily targeting the liver, spleen, lungs, and lower gastrointestinal tract. Establishing an animal LUJV model is an important first step towards understanding the high pathogenicity of LUJV and developing vaccines and antiviral therapeutic drugs for this highly transmissible and lethal emerging pathogen. |
format |
Article in Journal/Newspaper |
author |
Brian H Bird Kimberly A Dodd Bobbie R Erickson César G Albariño Ayan K Chakrabarti Laura K McMullan Eric Bergeron Ute Ströeher Deborah Cannon Brock Martin JoAnn D Coleman-McCray Stuart T Nichol Christina F Spiropoulou |
author_facet |
Brian H Bird Kimberly A Dodd Bobbie R Erickson César G Albariño Ayan K Chakrabarti Laura K McMullan Eric Bergeron Ute Ströeher Deborah Cannon Brock Martin JoAnn D Coleman-McCray Stuart T Nichol Christina F Spiropoulou |
author_sort |
Brian H Bird |
title |
Severe hemorrhagic fever in strain 13/N guinea pigs infected with Lujo virus. |
title_short |
Severe hemorrhagic fever in strain 13/N guinea pigs infected with Lujo virus. |
title_full |
Severe hemorrhagic fever in strain 13/N guinea pigs infected with Lujo virus. |
title_fullStr |
Severe hemorrhagic fever in strain 13/N guinea pigs infected with Lujo virus. |
title_full_unstemmed |
Severe hemorrhagic fever in strain 13/N guinea pigs infected with Lujo virus. |
title_sort |
severe hemorrhagic fever in strain 13/n guinea pigs infected with lujo virus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001801 https://doaj.org/article/f3f60ea17bdd4573b053e1114c5e9293 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 8, p e1801 (2012) |
op_relation |
http://europepmc.org/articles/PMC3429401?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 doi:10.1371/journal.pntd.0001801 1935-2727 1935-2735 https://doaj.org/article/f3f60ea17bdd4573b053e1114c5e9293 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001801 |
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PLOS Neglected Tropical Diseases |
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6 |
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8 |
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e1801 |
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