Curcuma angustifolia ameliorates carbon tetrachloride-induced hepatotoxicity in HepG2 cells and Swiss albino rats
Objective: To determine the antioxidant and hepatoprotective potential of methanol extract of rhizome of Curcuma angustifolia (MECA) against carbon tetrachloride (CCl4)-induced hepatic damage in vitro and in vivo. Methods: DPPH, ABTS and reducing power assays were performed to estimate the antioxida...
Published in: | Asian Pacific Journal of Tropical Medicine |
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:f3c81e31969844308283eedc5c878d61 2023-05-15T15:10:29+02:00 Curcuma angustifolia ameliorates carbon tetrachloride-induced hepatotoxicity in HepG2 cells and Swiss albino rats Sudipta Jena Asit Ray Diptirani Rath Ambika Sahoo Subhashree Singh Noohi Nasim Durga Madhab Kar Sanghamitra Nayak 2019-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.267585 https://doaj.org/article/f3c81e31969844308283eedc5c878d61 EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=9;spage=416;epage=424;aulast=Jena https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.267585 https://doaj.org/article/f3c81e31969844308283eedc5c878d61 Asian Pacific Journal of Tropical Medicine, Vol 12, Iss 9, Pp 416-424 (2019) curcuma angustifolia hepatoprotective activity carbon tetrachloride antioxidant activity hepg2 cells Arctic medicine. Tropical medicine RC955-962 article 2019 ftdoajarticles https://doi.org/10.4103/1995-7645.267585 2022-12-31T11:26:17Z Objective: To determine the antioxidant and hepatoprotective potential of methanol extract of rhizome of Curcuma angustifolia (MECA) against carbon tetrachloride (CCl4)-induced hepatic damage in vitro and in vivo. Methods: DPPH, ABTS and reducing power assays were performed to estimate the antioxidant effect of MECA. In vitro cytotoxicity of MECA against HepG2 cells was evaluated, whereas serum biochemical parameters and levels of antioxidative enzymes were measured in vivo and in vitro. Additionally, histopathological studies were estimated in order to investigate the hepatoprotective efficacy of MECA. Furthermore, GC-MS analysis of the extract was performed to identify the chemical components. Results: MECA exhibited strong antioxidant activity and attenuated CCl4-induced decrease in the viability of HepG2 cells. Additionally, MECA significantly restored the ALT, AST, ALP, TP and albumin level in comparison with the CCl4 group. After pre-treatment with MECA, effects of SOD, CAT and GSH were increased as well as lipid peroxidation amount decreased on CCl4-induced hepatotoxicity in in vitro and in vivo model. Furthermore, histopathological observation confirmed that MECA reduced liver injury induced by CCl4 in rats. GC-MS analysis confirmed the presence of bioactive constituents such as α-tocopherol (12.27%), phytol (7.61%), squalene (3.71%), β-sitosterol (2.19%), eugenol (2.59%), curcumenol (1.20%), β-elemene (1.00%) and eucalyptol (0.89%). Conclusions: MECA contains antioxidant and hepatoprotective constituents such asa-tocopherol, phytol, squalene and eugenol and exerts hepatoprotective effect both in vitro and in vivo. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 12 9 416 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
curcuma angustifolia hepatoprotective activity carbon tetrachloride antioxidant activity hepg2 cells Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
curcuma angustifolia hepatoprotective activity carbon tetrachloride antioxidant activity hepg2 cells Arctic medicine. Tropical medicine RC955-962 Sudipta Jena Asit Ray Diptirani Rath Ambika Sahoo Subhashree Singh Noohi Nasim Durga Madhab Kar Sanghamitra Nayak Curcuma angustifolia ameliorates carbon tetrachloride-induced hepatotoxicity in HepG2 cells and Swiss albino rats |
topic_facet |
curcuma angustifolia hepatoprotective activity carbon tetrachloride antioxidant activity hepg2 cells Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To determine the antioxidant and hepatoprotective potential of methanol extract of rhizome of Curcuma angustifolia (MECA) against carbon tetrachloride (CCl4)-induced hepatic damage in vitro and in vivo. Methods: DPPH, ABTS and reducing power assays were performed to estimate the antioxidant effect of MECA. In vitro cytotoxicity of MECA against HepG2 cells was evaluated, whereas serum biochemical parameters and levels of antioxidative enzymes were measured in vivo and in vitro. Additionally, histopathological studies were estimated in order to investigate the hepatoprotective efficacy of MECA. Furthermore, GC-MS analysis of the extract was performed to identify the chemical components. Results: MECA exhibited strong antioxidant activity and attenuated CCl4-induced decrease in the viability of HepG2 cells. Additionally, MECA significantly restored the ALT, AST, ALP, TP and albumin level in comparison with the CCl4 group. After pre-treatment with MECA, effects of SOD, CAT and GSH were increased as well as lipid peroxidation amount decreased on CCl4-induced hepatotoxicity in in vitro and in vivo model. Furthermore, histopathological observation confirmed that MECA reduced liver injury induced by CCl4 in rats. GC-MS analysis confirmed the presence of bioactive constituents such as α-tocopherol (12.27%), phytol (7.61%), squalene (3.71%), β-sitosterol (2.19%), eugenol (2.59%), curcumenol (1.20%), β-elemene (1.00%) and eucalyptol (0.89%). Conclusions: MECA contains antioxidant and hepatoprotective constituents such asa-tocopherol, phytol, squalene and eugenol and exerts hepatoprotective effect both in vitro and in vivo. |
format |
Article in Journal/Newspaper |
author |
Sudipta Jena Asit Ray Diptirani Rath Ambika Sahoo Subhashree Singh Noohi Nasim Durga Madhab Kar Sanghamitra Nayak |
author_facet |
Sudipta Jena Asit Ray Diptirani Rath Ambika Sahoo Subhashree Singh Noohi Nasim Durga Madhab Kar Sanghamitra Nayak |
author_sort |
Sudipta Jena |
title |
Curcuma angustifolia ameliorates carbon tetrachloride-induced hepatotoxicity in HepG2 cells and Swiss albino rats |
title_short |
Curcuma angustifolia ameliorates carbon tetrachloride-induced hepatotoxicity in HepG2 cells and Swiss albino rats |
title_full |
Curcuma angustifolia ameliorates carbon tetrachloride-induced hepatotoxicity in HepG2 cells and Swiss albino rats |
title_fullStr |
Curcuma angustifolia ameliorates carbon tetrachloride-induced hepatotoxicity in HepG2 cells and Swiss albino rats |
title_full_unstemmed |
Curcuma angustifolia ameliorates carbon tetrachloride-induced hepatotoxicity in HepG2 cells and Swiss albino rats |
title_sort |
curcuma angustifolia ameliorates carbon tetrachloride-induced hepatotoxicity in hepg2 cells and swiss albino rats |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/1995-7645.267585 https://doaj.org/article/f3c81e31969844308283eedc5c878d61 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 12, Iss 9, Pp 416-424 (2019) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=9;spage=416;epage=424;aulast=Jena https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.267585 https://doaj.org/article/f3c81e31969844308283eedc5c878d61 |
op_doi |
https://doi.org/10.4103/1995-7645.267585 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
12 |
container_issue |
9 |
container_start_page |
416 |
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1766341515573985280 |