The expression profile of a multi-stress inducible transient receptor potential vanilloid 4 (TRPV4) in Pacific oyster Crassostrea gigas
Transient receptor potential vanilloid 4 (TRPV4) is one of the major non-selective cation channel proteins, which plays a crucial role in sensing biotic and abiotic stresses, such as pathogen infection, temperature, mechanical pressure and osmotic pressure changes by regulating Ca2+ homeostasis. In...
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ftdoajarticles:oai:doaj.org/article:f3be7ac9969b432880de3e5ebfb17f3c 2023-05-15T15:57:56+02:00 The expression profile of a multi-stress inducible transient receptor potential vanilloid 4 (TRPV4) in Pacific oyster Crassostrea gigas Zhaoyu He Chuanyan Yang Dongli Jiang Xiangbo Wang Zhen Xing Simiao Yu Qian Yang Lingling Wang 2022-12-01T00:00:00Z https://doi.org/10.1016/j.fsirep.2022.100064 https://doaj.org/article/f3be7ac9969b432880de3e5ebfb17f3c EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2667011922000147 https://doaj.org/toc/2667-0119 2667-0119 doi:10.1016/j.fsirep.2022.100064 https://doaj.org/article/f3be7ac9969b432880de3e5ebfb17f3c Fish and Shellfish Immunology Reports, Vol 3, Iss , Pp 100064- (2022) Crassostrea gigas Transient receptor potential vanilloid 4 Expression profile High temperature stress Vibrio splendidus stimulation Zoology QL1-991 article 2022 ftdoajarticles https://doi.org/10.1016/j.fsirep.2022.100064 2022-12-30T22:25:44Z Transient receptor potential vanilloid 4 (TRPV4) is one of the major non-selective cation channel proteins, which plays a crucial role in sensing biotic and abiotic stresses, such as pathogen infection, temperature, mechanical pressure and osmotic pressure changes by regulating Ca2+ homeostasis. In the present study, a TRPV4 homologue was identified in Pacific oyster Crassostrea gigas, designated as CgTRPV4. The open reading frame (ORF) of CgTRPV4 was of 2298 bp encoding a putative polypeptide of 765 amino acid residues with three typical ankyrin domains and six conserved transmembrane domains of TRPV4 subfamily proteins, as well as multiple N-glycosylation sites, cAMP- and cGMP-dependent protein kinase phosphorylation sites, protein kinase C phosphorylation sites, casein kinase II phosphorylation sites, and prokaryotic membrane lipoprotein lipid attachment site. The deduced amino acid sequence of CgTRPV4 shared 20.5%-26.2% similarity with TRPV4s from other species. During the early ontogenesis stages of oyster, the mRNA transcripts of CgTRPV4 were detectable in all the stages with the highest expression level in fertilized eggs and the lowest in D-hinged larvae. In adult oyster, the CgTRPV4 mRNA could be detected in all the examined tissues, including gill, hepatopancreas, adductor muscle, labial palp, mantle and haemocyte, with the highest expression level in gill (45.08-fold of that in hepatopancreas, p < 0.05). In immunocytochemical assay, the CgTRPV4 positive signals were distributed in both endoplasmic reticulum and cytoplasmic membrane of oyster haemocytes. The mRNA expression of CgTRPV4 in gill was significantly up-regulated after high temperature stress at 30°C (p < 0.05) and after Vibrio splendidus stimulation (p < 0.05). These results indicated that CgTRPV4 was a classical member of TRPV4 family in oyster, which was induced by either biotic or abiotic stimulations and involved in mediating the stress response of oysters. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Fish and Shellfish Immunology Reports 3 100064 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Crassostrea gigas Transient receptor potential vanilloid 4 Expression profile High temperature stress Vibrio splendidus stimulation Zoology QL1-991 |
spellingShingle |
Crassostrea gigas Transient receptor potential vanilloid 4 Expression profile High temperature stress Vibrio splendidus stimulation Zoology QL1-991 Zhaoyu He Chuanyan Yang Dongli Jiang Xiangbo Wang Zhen Xing Simiao Yu Qian Yang Lingling Wang The expression profile of a multi-stress inducible transient receptor potential vanilloid 4 (TRPV4) in Pacific oyster Crassostrea gigas |
topic_facet |
Crassostrea gigas Transient receptor potential vanilloid 4 Expression profile High temperature stress Vibrio splendidus stimulation Zoology QL1-991 |
description |
Transient receptor potential vanilloid 4 (TRPV4) is one of the major non-selective cation channel proteins, which plays a crucial role in sensing biotic and abiotic stresses, such as pathogen infection, temperature, mechanical pressure and osmotic pressure changes by regulating Ca2+ homeostasis. In the present study, a TRPV4 homologue was identified in Pacific oyster Crassostrea gigas, designated as CgTRPV4. The open reading frame (ORF) of CgTRPV4 was of 2298 bp encoding a putative polypeptide of 765 amino acid residues with three typical ankyrin domains and six conserved transmembrane domains of TRPV4 subfamily proteins, as well as multiple N-glycosylation sites, cAMP- and cGMP-dependent protein kinase phosphorylation sites, protein kinase C phosphorylation sites, casein kinase II phosphorylation sites, and prokaryotic membrane lipoprotein lipid attachment site. The deduced amino acid sequence of CgTRPV4 shared 20.5%-26.2% similarity with TRPV4s from other species. During the early ontogenesis stages of oyster, the mRNA transcripts of CgTRPV4 were detectable in all the stages with the highest expression level in fertilized eggs and the lowest in D-hinged larvae. In adult oyster, the CgTRPV4 mRNA could be detected in all the examined tissues, including gill, hepatopancreas, adductor muscle, labial palp, mantle and haemocyte, with the highest expression level in gill (45.08-fold of that in hepatopancreas, p < 0.05). In immunocytochemical assay, the CgTRPV4 positive signals were distributed in both endoplasmic reticulum and cytoplasmic membrane of oyster haemocytes. The mRNA expression of CgTRPV4 in gill was significantly up-regulated after high temperature stress at 30°C (p < 0.05) and after Vibrio splendidus stimulation (p < 0.05). These results indicated that CgTRPV4 was a classical member of TRPV4 family in oyster, which was induced by either biotic or abiotic stimulations and involved in mediating the stress response of oysters. |
format |
Article in Journal/Newspaper |
author |
Zhaoyu He Chuanyan Yang Dongli Jiang Xiangbo Wang Zhen Xing Simiao Yu Qian Yang Lingling Wang |
author_facet |
Zhaoyu He Chuanyan Yang Dongli Jiang Xiangbo Wang Zhen Xing Simiao Yu Qian Yang Lingling Wang |
author_sort |
Zhaoyu He |
title |
The expression profile of a multi-stress inducible transient receptor potential vanilloid 4 (TRPV4) in Pacific oyster Crassostrea gigas |
title_short |
The expression profile of a multi-stress inducible transient receptor potential vanilloid 4 (TRPV4) in Pacific oyster Crassostrea gigas |
title_full |
The expression profile of a multi-stress inducible transient receptor potential vanilloid 4 (TRPV4) in Pacific oyster Crassostrea gigas |
title_fullStr |
The expression profile of a multi-stress inducible transient receptor potential vanilloid 4 (TRPV4) in Pacific oyster Crassostrea gigas |
title_full_unstemmed |
The expression profile of a multi-stress inducible transient receptor potential vanilloid 4 (TRPV4) in Pacific oyster Crassostrea gigas |
title_sort |
expression profile of a multi-stress inducible transient receptor potential vanilloid 4 (trpv4) in pacific oyster crassostrea gigas |
publisher |
Elsevier |
publishDate |
2022 |
url |
https://doi.org/10.1016/j.fsirep.2022.100064 https://doaj.org/article/f3be7ac9969b432880de3e5ebfb17f3c |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Fish and Shellfish Immunology Reports, Vol 3, Iss , Pp 100064- (2022) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2667011922000147 https://doaj.org/toc/2667-0119 2667-0119 doi:10.1016/j.fsirep.2022.100064 https://doaj.org/article/f3be7ac9969b432880de3e5ebfb17f3c |
op_doi |
https://doi.org/10.1016/j.fsirep.2022.100064 |
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Fish and Shellfish Immunology Reports |
container_volume |
3 |
container_start_page |
100064 |
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1766393642276093952 |