Cohort study of the association of antibody levels to AMA1, MSP1 19 , MSP3 and GLURP with protection from clinical malaria in Ghanaian children
Abstract Background Antigen-specific antibody-mediated immune responses play an important role in natural protection against clinical malaria, but conflicting estimates of this association have emerged from immuno-epidemiological studies in different geographical settings. This study was aimed at as...
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ftdoajarticles:oai:doaj.org/article:f39180b187694fc6b5c25412beea41e4 2023-05-15T15:18:02+02:00 Cohort study of the association of antibody levels to AMA1, MSP1 19 , MSP3 and GLURP with protection from clinical malaria in Ghanaian children Bosomprah Samuel Milligan Paul Remarque Ed Lamptey Helena Kusi Kwadwo Aikins Anastasia Dodoo Daniel Chilengi Roma Osei Yaa Difie Akanmori Bartholomew Theisen Michael 2008-07-01T00:00:00Z https://doi.org/10.1186/1475-2875-7-142 https://doaj.org/article/f39180b187694fc6b5c25412beea41e4 EN eng BMC http://www.malariajournal.com/content/7/1/142 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-142 1475-2875 https://doaj.org/article/f39180b187694fc6b5c25412beea41e4 Malaria Journal, Vol 7, Iss 1, p 142 (2008) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2008 ftdoajarticles https://doi.org/10.1186/1475-2875-7-142 2022-12-30T22:29:18Z Abstract Background Antigen-specific antibody-mediated immune responses play an important role in natural protection against clinical malaria, but conflicting estimates of this association have emerged from immuno-epidemiological studies in different geographical settings. This study was aimed at assessing in a standardized manner the relationship between the antibody responses to four malaria vaccine candidate antigens and protection from clinical malaria, in a cohort of Ghanaian children. Methods Standardized ELISA protocols were used to measure isotype and IgG subclass levels to Apical Membrane Antigen 1 (AMA1), Merozoite Surface Protein 1–19 (MSP1 19 ), Merozoite Surface Protein 3 (MSP3) and Glutamate Rich Protein (GLURP) antigens in plasma samples from 352 Ghanaian children, aged three to 10 years with subsequent malaria surveillance for nine months. This is one of a series of studies in different epidemiological settings using the same standardized ELISA protocols to permit comparisons of results from different laboratories. Results The incidence rate of malaria was 0.35 episodes per child per year. Isotype and IgG subclasses for all antigens investigated increased with age, while the risk of malaria decreased with age. After adjusting for age, higher levels of IgG to GLURP, MSP1 19 , MSP3 and IgM to MSP1 19 , MSP3 and AMA1 were associated with decreased malaria incidence. Of the IgG subclasses, only IgG1 to MSP1 19 was associated with reduced incidence of clinical malaria. A previous study in the same location failed to find an association of antibodies to MSP1 19 with clinical malaria. The disagreement may be due to differences in reagents, ELISA and analytical procedures used in the two studies. When IgG, IgM and IgG subclass levels for all four antigens were included in a combined model, only IgG1 [(0.80 (0.67–0.97), p = 0.018)] and IgM [(0.48 (0.32–0.72), p < 0.001)] to MSP1 19 were independently associated with protection from malaria. Conclusion Using standardized procedures, the study has ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 7 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Bosomprah Samuel Milligan Paul Remarque Ed Lamptey Helena Kusi Kwadwo Aikins Anastasia Dodoo Daniel Chilengi Roma Osei Yaa Difie Akanmori Bartholomew Theisen Michael Cohort study of the association of antibody levels to AMA1, MSP1 19 , MSP3 and GLURP with protection from clinical malaria in Ghanaian children |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Antigen-specific antibody-mediated immune responses play an important role in natural protection against clinical malaria, but conflicting estimates of this association have emerged from immuno-epidemiological studies in different geographical settings. This study was aimed at assessing in a standardized manner the relationship between the antibody responses to four malaria vaccine candidate antigens and protection from clinical malaria, in a cohort of Ghanaian children. Methods Standardized ELISA protocols were used to measure isotype and IgG subclass levels to Apical Membrane Antigen 1 (AMA1), Merozoite Surface Protein 1–19 (MSP1 19 ), Merozoite Surface Protein 3 (MSP3) and Glutamate Rich Protein (GLURP) antigens in plasma samples from 352 Ghanaian children, aged three to 10 years with subsequent malaria surveillance for nine months. This is one of a series of studies in different epidemiological settings using the same standardized ELISA protocols to permit comparisons of results from different laboratories. Results The incidence rate of malaria was 0.35 episodes per child per year. Isotype and IgG subclasses for all antigens investigated increased with age, while the risk of malaria decreased with age. After adjusting for age, higher levels of IgG to GLURP, MSP1 19 , MSP3 and IgM to MSP1 19 , MSP3 and AMA1 were associated with decreased malaria incidence. Of the IgG subclasses, only IgG1 to MSP1 19 was associated with reduced incidence of clinical malaria. A previous study in the same location failed to find an association of antibodies to MSP1 19 with clinical malaria. The disagreement may be due to differences in reagents, ELISA and analytical procedures used in the two studies. When IgG, IgM and IgG subclass levels for all four antigens were included in a combined model, only IgG1 [(0.80 (0.67–0.97), p = 0.018)] and IgM [(0.48 (0.32–0.72), p < 0.001)] to MSP1 19 were independently associated with protection from malaria. Conclusion Using standardized procedures, the study has ... |
format |
Article in Journal/Newspaper |
author |
Bosomprah Samuel Milligan Paul Remarque Ed Lamptey Helena Kusi Kwadwo Aikins Anastasia Dodoo Daniel Chilengi Roma Osei Yaa Difie Akanmori Bartholomew Theisen Michael |
author_facet |
Bosomprah Samuel Milligan Paul Remarque Ed Lamptey Helena Kusi Kwadwo Aikins Anastasia Dodoo Daniel Chilengi Roma Osei Yaa Difie Akanmori Bartholomew Theisen Michael |
author_sort |
Bosomprah Samuel |
title |
Cohort study of the association of antibody levels to AMA1, MSP1 19 , MSP3 and GLURP with protection from clinical malaria in Ghanaian children |
title_short |
Cohort study of the association of antibody levels to AMA1, MSP1 19 , MSP3 and GLURP with protection from clinical malaria in Ghanaian children |
title_full |
Cohort study of the association of antibody levels to AMA1, MSP1 19 , MSP3 and GLURP with protection from clinical malaria in Ghanaian children |
title_fullStr |
Cohort study of the association of antibody levels to AMA1, MSP1 19 , MSP3 and GLURP with protection from clinical malaria in Ghanaian children |
title_full_unstemmed |
Cohort study of the association of antibody levels to AMA1, MSP1 19 , MSP3 and GLURP with protection from clinical malaria in Ghanaian children |
title_sort |
cohort study of the association of antibody levels to ama1, msp1 19 , msp3 and glurp with protection from clinical malaria in ghanaian children |
publisher |
BMC |
publishDate |
2008 |
url |
https://doi.org/10.1186/1475-2875-7-142 https://doaj.org/article/f39180b187694fc6b5c25412beea41e4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 7, Iss 1, p 142 (2008) |
op_relation |
http://www.malariajournal.com/content/7/1/142 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-142 1475-2875 https://doaj.org/article/f39180b187694fc6b5c25412beea41e4 |
op_doi |
https://doi.org/10.1186/1475-2875-7-142 |
container_title |
Malaria Journal |
container_volume |
7 |
container_issue |
1 |
_version_ |
1766348265107750912 |