Biochemical and proteomics analyses of antioxidant enzymes reveal the potential stress tolerance in Rhododendron chrysanthum Pall
Abstract Background Rhododendron chrysanthum Pall., an endangered species with significant ornamental and medicinal value, is endemic to the Changbai Mountain of China and can also serve as a significant plant resource for investigating the stress tolerance in plants. Proteomics is an effective anal...
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ftdoajarticles:oai:doaj.org/article:f349af2cd77549aeadbe187b03aba444 2023-05-15T18:40:42+02:00 Biochemical and proteomics analyses of antioxidant enzymes reveal the potential stress tolerance in Rhododendron chrysanthum Pall Xiaofu Zhou Silin Chen Hui Wu Yi Yang Hongwei Xu 2017-05-01T00:00:00Z https://doi.org/10.1186/s13062-017-0181-6 https://doaj.org/article/f349af2cd77549aeadbe187b03aba444 EN eng BMC http://link.springer.com/article/10.1186/s13062-017-0181-6 https://doaj.org/toc/1745-6150 doi:10.1186/s13062-017-0181-6 1745-6150 https://doaj.org/article/f349af2cd77549aeadbe187b03aba444 Biology Direct, Vol 12, Iss 1, Pp 1-9 (2017) Rhododendron chrysanthum Pall Proteomics Antioxidant enzymes Stress tolerance Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1186/s13062-017-0181-6 2022-12-31T00:38:43Z Abstract Background Rhododendron chrysanthum Pall., an endangered species with significant ornamental and medicinal value, is endemic to the Changbai Mountain of China and can also serve as a significant plant resource for investigating the stress tolerance in plants. Proteomics is an effective analytical tool that provides significant information about plant metabolism and gene expression. However, no proteomics data have been reported for R. chrysanthum previously. In alpine tundra, the abiotic stress will lead to a severe over-accumulation of reactive oxygen species (ROS). Many alpine plants overcome the severe stresses and protect themselves from the oxidative damage by increasing the ratio and activity of antioxidant enzymes. Results In our study, wild type and domesticated Rhododendron chrysanthum Pall. were used as experimental and control groups, respectively. Proteomics method combined with biochemical approach were applied for the stress tolerance investigation of R. chrysanthum at both protein and molecular level. A total of 1,395 proteins were identified, among which 137 proteins were up-regulate in the experimental group. The activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidases (APXs), and glutathione peroxidase (GPX) were significantly higher and the expression of APXs and GPX were also increased in the experimental group. Moreover, the interaction network analysis of these enzymes also reveals that the antioxidant enzymes play important roles in the stress resistance in plants. Conclusions This is the first report of the proteome of Rhododendron chrysanthum Pall., and the data reinforce the notion that the antioxidant system plays a significant role in plant stress survival. Our results also verified that R. chrysanthum is highly resistant to abiotic stress and can serve as a significant resource for investigating stress tolerance in plants. Reviewers This article was reviewed by George V. (Yura) Shpakovski and Ramanathan Sowdhamini. Article in Journal/Newspaper Tundra Directory of Open Access Journals: DOAJ Articles Yura ENVELOPE(41.812,41.812,64.328,64.328) Biology Direct 12 1 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Rhododendron chrysanthum Pall Proteomics Antioxidant enzymes Stress tolerance Biology (General) QH301-705.5 |
spellingShingle |
Rhododendron chrysanthum Pall Proteomics Antioxidant enzymes Stress tolerance Biology (General) QH301-705.5 Xiaofu Zhou Silin Chen Hui Wu Yi Yang Hongwei Xu Biochemical and proteomics analyses of antioxidant enzymes reveal the potential stress tolerance in Rhododendron chrysanthum Pall |
topic_facet |
Rhododendron chrysanthum Pall Proteomics Antioxidant enzymes Stress tolerance Biology (General) QH301-705.5 |
description |
Abstract Background Rhododendron chrysanthum Pall., an endangered species with significant ornamental and medicinal value, is endemic to the Changbai Mountain of China and can also serve as a significant plant resource for investigating the stress tolerance in plants. Proteomics is an effective analytical tool that provides significant information about plant metabolism and gene expression. However, no proteomics data have been reported for R. chrysanthum previously. In alpine tundra, the abiotic stress will lead to a severe over-accumulation of reactive oxygen species (ROS). Many alpine plants overcome the severe stresses and protect themselves from the oxidative damage by increasing the ratio and activity of antioxidant enzymes. Results In our study, wild type and domesticated Rhododendron chrysanthum Pall. were used as experimental and control groups, respectively. Proteomics method combined with biochemical approach were applied for the stress tolerance investigation of R. chrysanthum at both protein and molecular level. A total of 1,395 proteins were identified, among which 137 proteins were up-regulate in the experimental group. The activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidases (APXs), and glutathione peroxidase (GPX) were significantly higher and the expression of APXs and GPX were also increased in the experimental group. Moreover, the interaction network analysis of these enzymes also reveals that the antioxidant enzymes play important roles in the stress resistance in plants. Conclusions This is the first report of the proteome of Rhododendron chrysanthum Pall., and the data reinforce the notion that the antioxidant system plays a significant role in plant stress survival. Our results also verified that R. chrysanthum is highly resistant to abiotic stress and can serve as a significant resource for investigating stress tolerance in plants. Reviewers This article was reviewed by George V. (Yura) Shpakovski and Ramanathan Sowdhamini. |
format |
Article in Journal/Newspaper |
author |
Xiaofu Zhou Silin Chen Hui Wu Yi Yang Hongwei Xu |
author_facet |
Xiaofu Zhou Silin Chen Hui Wu Yi Yang Hongwei Xu |
author_sort |
Xiaofu Zhou |
title |
Biochemical and proteomics analyses of antioxidant enzymes reveal the potential stress tolerance in Rhododendron chrysanthum Pall |
title_short |
Biochemical and proteomics analyses of antioxidant enzymes reveal the potential stress tolerance in Rhododendron chrysanthum Pall |
title_full |
Biochemical and proteomics analyses of antioxidant enzymes reveal the potential stress tolerance in Rhododendron chrysanthum Pall |
title_fullStr |
Biochemical and proteomics analyses of antioxidant enzymes reveal the potential stress tolerance in Rhododendron chrysanthum Pall |
title_full_unstemmed |
Biochemical and proteomics analyses of antioxidant enzymes reveal the potential stress tolerance in Rhododendron chrysanthum Pall |
title_sort |
biochemical and proteomics analyses of antioxidant enzymes reveal the potential stress tolerance in rhododendron chrysanthum pall |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s13062-017-0181-6 https://doaj.org/article/f349af2cd77549aeadbe187b03aba444 |
long_lat |
ENVELOPE(41.812,41.812,64.328,64.328) |
geographic |
Yura |
geographic_facet |
Yura |
genre |
Tundra |
genre_facet |
Tundra |
op_source |
Biology Direct, Vol 12, Iss 1, Pp 1-9 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s13062-017-0181-6 https://doaj.org/toc/1745-6150 doi:10.1186/s13062-017-0181-6 1745-6150 https://doaj.org/article/f349af2cd77549aeadbe187b03aba444 |
op_doi |
https://doi.org/10.1186/s13062-017-0181-6 |
container_title |
Biology Direct |
container_volume |
12 |
container_issue |
1 |
_version_ |
1766230112659832832 |