CD8+ T cells as a source of IFN-γ production in human cutaneous leishmaniasis.
BACKGROUND: In human leishmaniasis Th1/Th2 dichotomy similar to murine model is not clearly defined and surrogate marker(s) of protection is not yet known. In this study, Th1/Th2 cytokines (IL-5, IL-10, IL-13 and IFN-γ) profile induced by purified CD4(+)/CD8(+) T cells in response to Leishmania anti...
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ftdoajarticles:oai:doaj.org/article:f333415484cd41e38cb3fd35a98d03a4 2023-05-15T15:15:05+02:00 CD8+ T cells as a source of IFN-γ production in human cutaneous leishmaniasis. Mahmoud Nateghi Rostami Hossein Keshavarz Rosita Edalat Abdolfattah Sarrafnejad Tahereh Shahrestani Fereidoun Mahboudi Ali Khamesipour 2010-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000845 https://doaj.org/article/f333415484cd41e38cb3fd35a98d03a4 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2953482?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000845 https://doaj.org/article/f333415484cd41e38cb3fd35a98d03a4 PLoS Neglected Tropical Diseases, Vol 4, Iss 10, p e845 (2010) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2010 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000845 2022-12-31T03:38:12Z BACKGROUND: In human leishmaniasis Th1/Th2 dichotomy similar to murine model is not clearly defined and surrogate marker(s) of protection is not yet known. In this study, Th1/Th2 cytokines (IL-5, IL-10, IL-13 and IFN-γ) profile induced by purified CD4(+)/CD8(+) T cells in response to Leishmania antigens were assessed at transcript and protein levels in 14 volunteers with a history of self-healing cutaneous leishmaniasis (HCL) and compared with 18 healthy control volunteers. METHODOLOGY/PRINCIPAL FINDINGS: CD4(+)/CD8(+)/CD14(+) cells were purified from peripheral blood using magnetic beads; CD4(+)/CD8(+) T cells were co-cultured with autologous CD14(+) monocytes in the presence of soluble Leishmania antigens (SLA). Stimulation of either CD4(+) T cells or CD8(+) T cells of HCL volunteers with SLA induced a significantly (P<0.05) higher IFN-γ production compared with the cells of controls. Upregulation of IFN-γ gene expression in CD4(+) cells (P<0.001) and CD8(+) cells (P = 0.006) of HCL volunteers was significantly more than that of controls. Significantly (P<0.05) higher fold-expression of IFN-γ gene was seen in CD4(+) cells than in CD8(+) cells. In HCL volunteers a significantly (P = 0.014) higher number of CD4(+) cells were positive for intracellular IFN-γ production than CD8(+) cells. CONCLUSIONS/SIGNIFICANCE: Collectively, the volunteers have shown maintenance of specific long-term immune responses characterized by a strong reaction to leishmanin skin test and IFN-γ production. The dominant IFN-γ response was the result of expansion of both CD4(+) and CD8(+) T cells. The results suggested that immune response in protected individuals with a history of zoonotic cutaneous leishmaniasis (ZCL) due to L. major is mediated not only through the expansion of antigen-specific IFN-γ producing CD4(+) Th1 cells, but also through IFN-γ producing CD8(+) T cells. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 4 10 e845 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Mahmoud Nateghi Rostami Hossein Keshavarz Rosita Edalat Abdolfattah Sarrafnejad Tahereh Shahrestani Fereidoun Mahboudi Ali Khamesipour CD8+ T cells as a source of IFN-γ production in human cutaneous leishmaniasis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: In human leishmaniasis Th1/Th2 dichotomy similar to murine model is not clearly defined and surrogate marker(s) of protection is not yet known. In this study, Th1/Th2 cytokines (IL-5, IL-10, IL-13 and IFN-γ) profile induced by purified CD4(+)/CD8(+) T cells in response to Leishmania antigens were assessed at transcript and protein levels in 14 volunteers with a history of self-healing cutaneous leishmaniasis (HCL) and compared with 18 healthy control volunteers. METHODOLOGY/PRINCIPAL FINDINGS: CD4(+)/CD8(+)/CD14(+) cells were purified from peripheral blood using magnetic beads; CD4(+)/CD8(+) T cells were co-cultured with autologous CD14(+) monocytes in the presence of soluble Leishmania antigens (SLA). Stimulation of either CD4(+) T cells or CD8(+) T cells of HCL volunteers with SLA induced a significantly (P<0.05) higher IFN-γ production compared with the cells of controls. Upregulation of IFN-γ gene expression in CD4(+) cells (P<0.001) and CD8(+) cells (P = 0.006) of HCL volunteers was significantly more than that of controls. Significantly (P<0.05) higher fold-expression of IFN-γ gene was seen in CD4(+) cells than in CD8(+) cells. In HCL volunteers a significantly (P = 0.014) higher number of CD4(+) cells were positive for intracellular IFN-γ production than CD8(+) cells. CONCLUSIONS/SIGNIFICANCE: Collectively, the volunteers have shown maintenance of specific long-term immune responses characterized by a strong reaction to leishmanin skin test and IFN-γ production. The dominant IFN-γ response was the result of expansion of both CD4(+) and CD8(+) T cells. The results suggested that immune response in protected individuals with a history of zoonotic cutaneous leishmaniasis (ZCL) due to L. major is mediated not only through the expansion of antigen-specific IFN-γ producing CD4(+) Th1 cells, but also through IFN-γ producing CD8(+) T cells. |
format |
Article in Journal/Newspaper |
author |
Mahmoud Nateghi Rostami Hossein Keshavarz Rosita Edalat Abdolfattah Sarrafnejad Tahereh Shahrestani Fereidoun Mahboudi Ali Khamesipour |
author_facet |
Mahmoud Nateghi Rostami Hossein Keshavarz Rosita Edalat Abdolfattah Sarrafnejad Tahereh Shahrestani Fereidoun Mahboudi Ali Khamesipour |
author_sort |
Mahmoud Nateghi Rostami |
title |
CD8+ T cells as a source of IFN-γ production in human cutaneous leishmaniasis. |
title_short |
CD8+ T cells as a source of IFN-γ production in human cutaneous leishmaniasis. |
title_full |
CD8+ T cells as a source of IFN-γ production in human cutaneous leishmaniasis. |
title_fullStr |
CD8+ T cells as a source of IFN-γ production in human cutaneous leishmaniasis. |
title_full_unstemmed |
CD8+ T cells as a source of IFN-γ production in human cutaneous leishmaniasis. |
title_sort |
cd8+ t cells as a source of ifn-γ production in human cutaneous leishmaniasis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2010 |
url |
https://doi.org/10.1371/journal.pntd.0000845 https://doaj.org/article/f333415484cd41e38cb3fd35a98d03a4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 4, Iss 10, p e845 (2010) |
op_relation |
http://europepmc.org/articles/PMC2953482?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000845 https://doaj.org/article/f333415484cd41e38cb3fd35a98d03a4 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000845 |
container_title |
PLoS Neglected Tropical Diseases |
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4 |
container_issue |
10 |
container_start_page |
e845 |
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1766345458961088512 |