Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells.
Clinical manifestations in onchocerciasis range from generalized onchocerciasis (GEO) to the rare but severe hyperreactive (HO)/sowda form. Since disease pathogenesis is associated with host inflammatory reactions, we investigated whether Th17 responses could be related to aggravated pathology in HO...
Published in: | PLoS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0003414 https://doaj.org/article/f2da924f6cba47eaa0e98b3bbc948e8c |
id |
ftdoajarticles:oai:doaj.org/article:f2da924f6cba47eaa0e98b3bbc948e8c |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:f2da924f6cba47eaa0e98b3bbc948e8c 2023-05-15T15:08:09+02:00 Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells. Gnatoulma Katawa Laura E Layland Alex Y Debrah Charlotte von Horn Linda Batsa Alexander Kwarteng Sandra Arriens David W Taylor Sabine Specht Achim Hoerauf Tomabu Adjobimey 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003414 https://doaj.org/article/f2da924f6cba47eaa0e98b3bbc948e8c EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4288720?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003414 https://doaj.org/article/f2da924f6cba47eaa0e98b3bbc948e8c PLoS Neglected Tropical Diseases, Vol 9, Iss 1, p e3414 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003414 2022-12-31T05:33:50Z Clinical manifestations in onchocerciasis range from generalized onchocerciasis (GEO) to the rare but severe hyperreactive (HO)/sowda form. Since disease pathogenesis is associated with host inflammatory reactions, we investigated whether Th17 responses could be related to aggravated pathology in HO. Using flow cytometry, filarial-specific cytokine responses and PCR arrays, we compared the immune cell profiles, including Th subsets, in individuals presenting the two polar forms of infection and endemic normals (EN). In addition to elevated frequencies of memory CD4+ T cells, individuals with HO showed accentuated Th17 and Th2 profiles but decreased CD4+CD25hiFoxp3+ regulatory T cells. These profiles included increased IL-17A+, IL-4+, RORC2+ and GATA3+CD4+ T cell populations. Flow cytometry data was further confirmed using a PCR array since Th17-related genes (IL-17 family members, IL-6, IL-1β and IL-22) and Th2-related (IL-4, IL-13, STAT6) genes were all significantly up-regulated in HO individuals. In addition, stronger Onchocerca volvulus-specific Th2 responses, especially IL-13, were observed in vitro in hyperreactive individuals when compared to GEO or EN groups. This study provides initial evidence that elevated frequencies of Th17 and Th2 cells form part of the immune network instigating the development of severe onchocerciasis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 9 1 e3414 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Gnatoulma Katawa Laura E Layland Alex Y Debrah Charlotte von Horn Linda Batsa Alexander Kwarteng Sandra Arriens David W Taylor Sabine Specht Achim Hoerauf Tomabu Adjobimey Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Clinical manifestations in onchocerciasis range from generalized onchocerciasis (GEO) to the rare but severe hyperreactive (HO)/sowda form. Since disease pathogenesis is associated with host inflammatory reactions, we investigated whether Th17 responses could be related to aggravated pathology in HO. Using flow cytometry, filarial-specific cytokine responses and PCR arrays, we compared the immune cell profiles, including Th subsets, in individuals presenting the two polar forms of infection and endemic normals (EN). In addition to elevated frequencies of memory CD4+ T cells, individuals with HO showed accentuated Th17 and Th2 profiles but decreased CD4+CD25hiFoxp3+ regulatory T cells. These profiles included increased IL-17A+, IL-4+, RORC2+ and GATA3+CD4+ T cell populations. Flow cytometry data was further confirmed using a PCR array since Th17-related genes (IL-17 family members, IL-6, IL-1β and IL-22) and Th2-related (IL-4, IL-13, STAT6) genes were all significantly up-regulated in HO individuals. In addition, stronger Onchocerca volvulus-specific Th2 responses, especially IL-13, were observed in vitro in hyperreactive individuals when compared to GEO or EN groups. This study provides initial evidence that elevated frequencies of Th17 and Th2 cells form part of the immune network instigating the development of severe onchocerciasis. |
format |
Article in Journal/Newspaper |
author |
Gnatoulma Katawa Laura E Layland Alex Y Debrah Charlotte von Horn Linda Batsa Alexander Kwarteng Sandra Arriens David W Taylor Sabine Specht Achim Hoerauf Tomabu Adjobimey |
author_facet |
Gnatoulma Katawa Laura E Layland Alex Y Debrah Charlotte von Horn Linda Batsa Alexander Kwarteng Sandra Arriens David W Taylor Sabine Specht Achim Hoerauf Tomabu Adjobimey |
author_sort |
Gnatoulma Katawa |
title |
Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells. |
title_short |
Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells. |
title_full |
Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells. |
title_fullStr |
Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells. |
title_full_unstemmed |
Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells. |
title_sort |
hyperreactive onchocerciasis is characterized by a combination of th17-th2 immune responses and reduced regulatory t cells. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0003414 https://doaj.org/article/f2da924f6cba47eaa0e98b3bbc948e8c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 1, p e3414 (2015) |
op_relation |
http://europepmc.org/articles/PMC4288720?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003414 https://doaj.org/article/f2da924f6cba47eaa0e98b3bbc948e8c |
op_doi |
https://doi.org/10.1371/journal.pntd.0003414 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
9 |
container_issue |
1 |
container_start_page |
e3414 |
_version_ |
1766339559760592896 |