Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice
Abstract INTRODUCTION: Salmonella enterica serotype Enteritidis (S. Enteritidis) is a cause of food-borne human illness. Given the prevalence of antibiotic resistance of Salmonella Enteritidis and the lack of antibiotic efficacy in future years, its replacement with other agents is necessary. One of...
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ftdoajarticles:oai:doaj.org/article:f2c67fa6c717465e9906f3fcbd676e36 2023-05-15T15:05:26+02:00 Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice Mohammad Mehdi Soltan Dallal Farhad Nikkhahi Mahmoud Alimohammadi Masoumeh Douraghi Zahra Rajabi Abbas Rahimi Foroushani Akram Azimi Fatemeh Fardsanei 2019-11-01T00:00:00Z https://doi.org/10.1590/0037-8682-0290-2019 https://doaj.org/article/f2c67fa6c717465e9906f3fcbd676e36 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100342&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0290-2019 https://doaj.org/article/f2c67fa6c717465e9906f3fcbd676e36 Revista da Sociedade Brasileira de Medicina Tropical, Vol 52 (2019) Salmonella enterica serotype Enteritidis Phage SE20 Pulsed-field gel electrophoresis Mice Sewage Arctic medicine. Tropical medicine RC955-962 article 2019 ftdoajarticles https://doi.org/10.1590/0037-8682-0290-2019 2022-12-30T23:24:18Z Abstract INTRODUCTION: Salmonella enterica serotype Enteritidis (S. Enteritidis) is a cause of food-borne human illness. Given the prevalence of antibiotic resistance of Salmonella Enteritidis and the lack of antibiotic efficacy in future years, its replacement with other agents is necessary. One of the most useful agents is bacteriophages. METHODS S. Enteritidis was identified using a multiplex polymerase chain reaction assay. The effective bacteriophages were isolated from hospital wastewater samples. The effects of the bacteriophages were evaluated both in vitro and in vivo. RESULTS The phage SE20 belonged to the Podoviridae family, and the genome size was 40 kb. The evaluation of phage SE20 at variable pH ranges showed its susceptibility to pH < 3 and pH > 12. The animal model showed that mice infected with S. Enteritidis developed hepatomegaly and splenomegaly, but did not experience gastrointestinal complications after receiving the bacteriophages. CONCLUSIONS The results of this study suggest that phage SE20 is a promising candidate for controlling salmonellosis caused by Salmonella Enteritidis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 52 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Salmonella enterica serotype Enteritidis Phage SE20 Pulsed-field gel electrophoresis Mice Sewage Arctic medicine. Tropical medicine RC955-962 |
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Salmonella enterica serotype Enteritidis Phage SE20 Pulsed-field gel electrophoresis Mice Sewage Arctic medicine. Tropical medicine RC955-962 Mohammad Mehdi Soltan Dallal Farhad Nikkhahi Mahmoud Alimohammadi Masoumeh Douraghi Zahra Rajabi Abbas Rahimi Foroushani Akram Azimi Fatemeh Fardsanei Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice |
topic_facet |
Salmonella enterica serotype Enteritidis Phage SE20 Pulsed-field gel electrophoresis Mice Sewage Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract INTRODUCTION: Salmonella enterica serotype Enteritidis (S. Enteritidis) is a cause of food-borne human illness. Given the prevalence of antibiotic resistance of Salmonella Enteritidis and the lack of antibiotic efficacy in future years, its replacement with other agents is necessary. One of the most useful agents is bacteriophages. METHODS S. Enteritidis was identified using a multiplex polymerase chain reaction assay. The effective bacteriophages were isolated from hospital wastewater samples. The effects of the bacteriophages were evaluated both in vitro and in vivo. RESULTS The phage SE20 belonged to the Podoviridae family, and the genome size was 40 kb. The evaluation of phage SE20 at variable pH ranges showed its susceptibility to pH < 3 and pH > 12. The animal model showed that mice infected with S. Enteritidis developed hepatomegaly and splenomegaly, but did not experience gastrointestinal complications after receiving the bacteriophages. CONCLUSIONS The results of this study suggest that phage SE20 is a promising candidate for controlling salmonellosis caused by Salmonella Enteritidis. |
format |
Article in Journal/Newspaper |
author |
Mohammad Mehdi Soltan Dallal Farhad Nikkhahi Mahmoud Alimohammadi Masoumeh Douraghi Zahra Rajabi Abbas Rahimi Foroushani Akram Azimi Fatemeh Fardsanei |
author_facet |
Mohammad Mehdi Soltan Dallal Farhad Nikkhahi Mahmoud Alimohammadi Masoumeh Douraghi Zahra Rajabi Abbas Rahimi Foroushani Akram Azimi Fatemeh Fardsanei |
author_sort |
Mohammad Mehdi Soltan Dallal |
title |
Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice |
title_short |
Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice |
title_full |
Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice |
title_fullStr |
Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice |
title_full_unstemmed |
Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice |
title_sort |
phage therapy as an approach to control salmonella enterica serotype enteritidis infection in mice |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2019 |
url |
https://doi.org/10.1590/0037-8682-0290-2019 https://doaj.org/article/f2c67fa6c717465e9906f3fcbd676e36 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 52 (2019) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100342&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0290-2019 https://doaj.org/article/f2c67fa6c717465e9906f3fcbd676e36 |
op_doi |
https://doi.org/10.1590/0037-8682-0290-2019 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
52 |
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