Polyketide and benzopyran compounds of an endophytic fungus isolated from C innamomum mollissimum: biological activity and structure
Objective: To study bioactivity and compounds produced by an endophytic Phoma sp. fungus isolated from the medicinal plant Cinnamomum mollissimum. Methods: Compounds produced by the fungus were extracted from fungal broth culture with ethyl acetate. This was followed by bioactivity profiling of the...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:f25aa20862ea483b85f01f3f0ecc290f 2023-05-15T15:09:23+02:00 Polyketide and benzopyran compounds of an endophytic fungus isolated from C innamomum mollissimum: biological activity and structure Carolina Santiago Lin Sun Murray Herbert Gibson Munro Jacinta Santhanam 2014-08-01T00:00:00Z https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0030 https://doaj.org/article/f25aa20862ea483b85f01f3f0ecc290f EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115300782 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014APJTB-2014-0030 https://doaj.org/article/f25aa20862ea483b85f01f3f0ecc290f Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 8, Pp 627-632 (2014) Endophytic fungi HPLC bioactivity profiling Cinnamomum mollissimum Antimicrobial Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0030 2022-12-31T03:00:21Z Objective: To study bioactivity and compounds produced by an endophytic Phoma sp. fungus isolated from the medicinal plant Cinnamomum mollissimum. Methods: Compounds produced by the fungus were extracted from fungal broth culture with ethyl acetate. This was followed by bioactivity profiling of the crude extract fractions obtained via high performance liquid chromatography. The fractions were tested for cytotoxicity to P388 murine leukemic cells and antimicrobial activity against bacteria and pathogenic fungi. Compounds purified from active fractions which showed antibacterial, antifungal and cytotoxic activities were identified using capillary nuclear magnetic resonance analysis, mass spectrometry and admission to AntiMarin database. Results: Three known compounds, namely 4-hydroxymellein, 4,8-dihydroxy-6-methoxy-3-methyl-3,4-dihydro-1H-isochromen-1-one and 1-(2,6-dihydroxyphenyl) ethanone, were isolated from the fungus. The polyketide compound 4-hydroxymellein showed high inhibitory activity against P388 murine leukemic cells (94.6%) and the bacteria Bacillus subtilis (97.3%). Meanwhile, 4,8-dihydroxy-6-methoxy-3-methyl-3,4-dihydro-1H-isochromen-1-one, a benzopyran compound, demonstrated moderate inhibitory activity against P388 murine leukemic cells (48.8%) and the fungus Aspergillus niger (56.1%). The second polyketide compound, 1 (2,6-dihydroxyphenyl) ethanone was inactive against the tested targets. Conclusions: These findings demonstrate the potential of endophytes as producers of pharmacologically important compounds, including polyketides which are major secondary metabolites in fungi. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 8 627 632 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Endophytic fungi HPLC bioactivity profiling Cinnamomum mollissimum Antimicrobial Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Endophytic fungi HPLC bioactivity profiling Cinnamomum mollissimum Antimicrobial Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Carolina Santiago Lin Sun Murray Herbert Gibson Munro Jacinta Santhanam Polyketide and benzopyran compounds of an endophytic fungus isolated from C innamomum mollissimum: biological activity and structure |
topic_facet |
Endophytic fungi HPLC bioactivity profiling Cinnamomum mollissimum Antimicrobial Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To study bioactivity and compounds produced by an endophytic Phoma sp. fungus isolated from the medicinal plant Cinnamomum mollissimum. Methods: Compounds produced by the fungus were extracted from fungal broth culture with ethyl acetate. This was followed by bioactivity profiling of the crude extract fractions obtained via high performance liquid chromatography. The fractions were tested for cytotoxicity to P388 murine leukemic cells and antimicrobial activity against bacteria and pathogenic fungi. Compounds purified from active fractions which showed antibacterial, antifungal and cytotoxic activities were identified using capillary nuclear magnetic resonance analysis, mass spectrometry and admission to AntiMarin database. Results: Three known compounds, namely 4-hydroxymellein, 4,8-dihydroxy-6-methoxy-3-methyl-3,4-dihydro-1H-isochromen-1-one and 1-(2,6-dihydroxyphenyl) ethanone, were isolated from the fungus. The polyketide compound 4-hydroxymellein showed high inhibitory activity against P388 murine leukemic cells (94.6%) and the bacteria Bacillus subtilis (97.3%). Meanwhile, 4,8-dihydroxy-6-methoxy-3-methyl-3,4-dihydro-1H-isochromen-1-one, a benzopyran compound, demonstrated moderate inhibitory activity against P388 murine leukemic cells (48.8%) and the fungus Aspergillus niger (56.1%). The second polyketide compound, 1 (2,6-dihydroxyphenyl) ethanone was inactive against the tested targets. Conclusions: These findings demonstrate the potential of endophytes as producers of pharmacologically important compounds, including polyketides which are major secondary metabolites in fungi. |
format |
Article in Journal/Newspaper |
author |
Carolina Santiago Lin Sun Murray Herbert Gibson Munro Jacinta Santhanam |
author_facet |
Carolina Santiago Lin Sun Murray Herbert Gibson Munro Jacinta Santhanam |
author_sort |
Carolina Santiago |
title |
Polyketide and benzopyran compounds of an endophytic fungus isolated from C innamomum mollissimum: biological activity and structure |
title_short |
Polyketide and benzopyran compounds of an endophytic fungus isolated from C innamomum mollissimum: biological activity and structure |
title_full |
Polyketide and benzopyran compounds of an endophytic fungus isolated from C innamomum mollissimum: biological activity and structure |
title_fullStr |
Polyketide and benzopyran compounds of an endophytic fungus isolated from C innamomum mollissimum: biological activity and structure |
title_full_unstemmed |
Polyketide and benzopyran compounds of an endophytic fungus isolated from C innamomum mollissimum: biological activity and structure |
title_sort |
polyketide and benzopyran compounds of an endophytic fungus isolated from c innamomum mollissimum: biological activity and structure |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0030 https://doaj.org/article/f25aa20862ea483b85f01f3f0ecc290f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 8, Pp 627-632 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115300782 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014APJTB-2014-0030 https://doaj.org/article/f25aa20862ea483b85f01f3f0ecc290f |
op_doi |
https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0030 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_issue |
8 |
container_start_page |
627 |
op_container_end_page |
632 |
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1766340590773993472 |