REPRODUCIBILITY OF ALKALINE ANTIGENS OF Leishmania major-LIKE AND Leishmania (V.) braziliensis EVALUATED BY IgG-ELISA. COMPARISON OF ANTIGENS ADDED OF A PROTEIN INHIBITOR (PMSF) OR NOT
This paper deals with the analysis of 10 batches of L.major-like and L.(V.) braziliensis antigens added or not of a proteases inhibitor evaluated by means of an IgG-ELISA on three consecutive days using positive standard sera from patients with diagnosis of American Leishmaniasis previously tested f...
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Universidade de São Paulo (USP)
1998
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ftdoajarticles:oai:doaj.org/article:f20241566ffb447f8edc333335659a0d 2024-09-09T19:28:33+00:00 REPRODUCIBILITY OF ALKALINE ANTIGENS OF Leishmania major-LIKE AND Leishmania (V.) braziliensis EVALUATED BY IgG-ELISA. COMPARISON OF ANTIGENS ADDED OF A PROTEIN INHIBITOR (PMSF) OR NOT Beatriz Julieta Celeste Maria Carolina Soares Guimarães José Maria Pacheco de Souza Denise P. Bergamaschi 1998-09-01T00:00:00Z https://doi.org/10.1590/S0036-46651998000500004 https://doaj.org/article/f20241566ffb447f8edc333335659a0d EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651998000500004 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651998000500004 0036-4665 1678-9946 https://doaj.org/article/f20241566ffb447f8edc333335659a0d Revista do Instituto de Medicina Tropical de São Paulo, Vol 40, Iss 5 (1998) Reproducibility of serological tests Alkaline antigens Leishmania major-like Leishmania (V.) braziliensis IgG-ELISA Protein inhibitor (PMSF) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1998 ftdoajarticles https://doi.org/10.1590/S0036-46651998000500004 2024-08-05T17:49:31Z This paper deals with the analysis of 10 batches of L.major-like and L.(V.) braziliensis antigens added or not of a proteases inhibitor evaluated by means of an IgG-ELISA on three consecutive days using positive standard sera from patients with diagnosis of American Leishmaniasis previously tested for the presence of IgG antibodies by means of ELISA. The statistical analysis showed that for L. (V.) braziliensis the PMSF-containing antigen did not show any difference among batches or days of testing; the L.(V.) braziliensis antigen without PMSF showed statistical significance for differences among batches and a two-way ANOVA showed significant differences between antigens. L.major-like antigen prepared with or without PMSF showed differences among batches; all 3 days of testing displayed differences for the PMSF antigen but only for days 1 and 2 for the antigen without inhibitor. A two-way ANOVA showed differences among batches of the antigens but not for antigens with and without the protein inhibitor. According to the statistical analysis the L.major-like antigen added or not of PMSF has shown that it is the choice antigen for mucocutaneous leishmaniasis serology. Neste trabalho estudou-se o comportamento de 10 partidas de antígenos alcalinos de L.major-like e L.(V.) braziliensis adicionados ou não de um inibidor de proteases (PMSF) avaliados em tres dias consecutivos por meio de ELISA-IgG empregando soros padrão positivo de pacientes com diagnóstico de leishmaniose mucocutânea previamente testados para a presença de anticorpos anti-leishmania por ELISA. A análise estatística mostrou que para o antígeno de L.(V.) braziliensis adicionado de PMSF não houve diferença significante entre as partidas ou dias de teste. Para o antígeno sem PMSF houve diferença entre as partidas mas não entre os dias de teste. Uma ANOVA bi-caudal mostrou diferenças entre os antígenos com e sem PMSF. Os antígeno de L.major-like preparados com e sem adição de PMSF mostraram diferenças significativas entre as partidas; os tres dias de ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 40 5 287 290 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Reproducibility of serological tests Alkaline antigens Leishmania major-like Leishmania (V.) braziliensis IgG-ELISA Protein inhibitor (PMSF) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Reproducibility of serological tests Alkaline antigens Leishmania major-like Leishmania (V.) braziliensis IgG-ELISA Protein inhibitor (PMSF) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Beatriz Julieta Celeste Maria Carolina Soares Guimarães José Maria Pacheco de Souza Denise P. Bergamaschi REPRODUCIBILITY OF ALKALINE ANTIGENS OF Leishmania major-LIKE AND Leishmania (V.) braziliensis EVALUATED BY IgG-ELISA. COMPARISON OF ANTIGENS ADDED OF A PROTEIN INHIBITOR (PMSF) OR NOT |
topic_facet |
Reproducibility of serological tests Alkaline antigens Leishmania major-like Leishmania (V.) braziliensis IgG-ELISA Protein inhibitor (PMSF) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
This paper deals with the analysis of 10 batches of L.major-like and L.(V.) braziliensis antigens added or not of a proteases inhibitor evaluated by means of an IgG-ELISA on three consecutive days using positive standard sera from patients with diagnosis of American Leishmaniasis previously tested for the presence of IgG antibodies by means of ELISA. The statistical analysis showed that for L. (V.) braziliensis the PMSF-containing antigen did not show any difference among batches or days of testing; the L.(V.) braziliensis antigen without PMSF showed statistical significance for differences among batches and a two-way ANOVA showed significant differences between antigens. L.major-like antigen prepared with or without PMSF showed differences among batches; all 3 days of testing displayed differences for the PMSF antigen but only for days 1 and 2 for the antigen without inhibitor. A two-way ANOVA showed differences among batches of the antigens but not for antigens with and without the protein inhibitor. According to the statistical analysis the L.major-like antigen added or not of PMSF has shown that it is the choice antigen for mucocutaneous leishmaniasis serology. Neste trabalho estudou-se o comportamento de 10 partidas de antígenos alcalinos de L.major-like e L.(V.) braziliensis adicionados ou não de um inibidor de proteases (PMSF) avaliados em tres dias consecutivos por meio de ELISA-IgG empregando soros padrão positivo de pacientes com diagnóstico de leishmaniose mucocutânea previamente testados para a presença de anticorpos anti-leishmania por ELISA. A análise estatística mostrou que para o antígeno de L.(V.) braziliensis adicionado de PMSF não houve diferença significante entre as partidas ou dias de teste. Para o antígeno sem PMSF houve diferença entre as partidas mas não entre os dias de teste. Uma ANOVA bi-caudal mostrou diferenças entre os antígenos com e sem PMSF. Os antígeno de L.major-like preparados com e sem adição de PMSF mostraram diferenças significativas entre as partidas; os tres dias de ... |
format |
Article in Journal/Newspaper |
author |
Beatriz Julieta Celeste Maria Carolina Soares Guimarães José Maria Pacheco de Souza Denise P. Bergamaschi |
author_facet |
Beatriz Julieta Celeste Maria Carolina Soares Guimarães José Maria Pacheco de Souza Denise P. Bergamaschi |
author_sort |
Beatriz Julieta Celeste |
title |
REPRODUCIBILITY OF ALKALINE ANTIGENS OF Leishmania major-LIKE AND Leishmania (V.) braziliensis EVALUATED BY IgG-ELISA. COMPARISON OF ANTIGENS ADDED OF A PROTEIN INHIBITOR (PMSF) OR NOT |
title_short |
REPRODUCIBILITY OF ALKALINE ANTIGENS OF Leishmania major-LIKE AND Leishmania (V.) braziliensis EVALUATED BY IgG-ELISA. COMPARISON OF ANTIGENS ADDED OF A PROTEIN INHIBITOR (PMSF) OR NOT |
title_full |
REPRODUCIBILITY OF ALKALINE ANTIGENS OF Leishmania major-LIKE AND Leishmania (V.) braziliensis EVALUATED BY IgG-ELISA. COMPARISON OF ANTIGENS ADDED OF A PROTEIN INHIBITOR (PMSF) OR NOT |
title_fullStr |
REPRODUCIBILITY OF ALKALINE ANTIGENS OF Leishmania major-LIKE AND Leishmania (V.) braziliensis EVALUATED BY IgG-ELISA. COMPARISON OF ANTIGENS ADDED OF A PROTEIN INHIBITOR (PMSF) OR NOT |
title_full_unstemmed |
REPRODUCIBILITY OF ALKALINE ANTIGENS OF Leishmania major-LIKE AND Leishmania (V.) braziliensis EVALUATED BY IgG-ELISA. COMPARISON OF ANTIGENS ADDED OF A PROTEIN INHIBITOR (PMSF) OR NOT |
title_sort |
reproducibility of alkaline antigens of leishmania major-like and leishmania (v.) braziliensis evaluated by igg-elisa. comparison of antigens added of a protein inhibitor (pmsf) or not |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1998 |
url |
https://doi.org/10.1590/S0036-46651998000500004 https://doaj.org/article/f20241566ffb447f8edc333335659a0d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 40, Iss 5 (1998) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651998000500004 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651998000500004 0036-4665 1678-9946 https://doaj.org/article/f20241566ffb447f8edc333335659a0d |
op_doi |
https://doi.org/10.1590/S0036-46651998000500004 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
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40 |
container_issue |
5 |
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287 |
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