Heterologous production and characterization of a pyomelanin of Antarctic Pseudomonas sp. ANT_H4: a metabolite protecting against UV and free radicals, interacting with iron from minerals and exhibiting priming properties toward plant hairy roots
Abstract Background Antarctica has one of the most extreme environments in the world. This region is inhabited by specifically adapted microorganisms that produce various unique secondary metabolites (e.g. pigments) enabling their survival under the harsh environmental conditions. It was already sho...
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ftdoajarticles:oai:doaj.org/article:f13a3b6acef24787b8504e6c037ce058 2023-05-15T13:50:55+02:00 Heterologous production and characterization of a pyomelanin of Antarctic Pseudomonas sp. ANT_H4: a metabolite protecting against UV and free radicals, interacting with iron from minerals and exhibiting priming properties toward plant hairy roots Michal Styczynski Agata Rogowska Christine Nyabayo Przemyslaw Decewicz Filip Romaniuk Cezary Pączkowski Anna Szakiel Roderich Suessmuth Lukasz Dziewit 2022-12-01T00:00:00Z https://doi.org/10.1186/s12934-022-01990-3 https://doaj.org/article/f13a3b6acef24787b8504e6c037ce058 EN eng BMC https://doi.org/10.1186/s12934-022-01990-3 https://doaj.org/toc/1475-2859 doi:10.1186/s12934-022-01990-3 1475-2859 https://doaj.org/article/f13a3b6acef24787b8504e6c037ce058 Microbial Cell Factories, Vol 21, Iss 1, Pp 1-17 (2022) Antarctica Biopolymer Pseudomonas Priming of hairy roots Pyomelanin Microbiology QR1-502 article 2022 ftdoajarticles https://doi.org/10.1186/s12934-022-01990-3 2022-12-30T22:23:13Z Abstract Background Antarctica has one of the most extreme environments in the world. This region is inhabited by specifically adapted microorganisms that produce various unique secondary metabolites (e.g. pigments) enabling their survival under the harsh environmental conditions. It was already shown that these natural, biologically active molecules may find application in various fields of biotechnology. Results In this study, a cold-active brown-pigment-producing Pseudomonas sp. ANT_H4 strain was characterized. In-depth genomic analysis combined with the application of a fosmid expression system revealed two different pathways of melanin-like compounds biosynthesis by the ANT_H4 strain. The chromatographic behavior and Fourier-transform infrared spectroscopic analyses allowed for the identification of the extracted melanin-like compound as a pyomelanin. Furthermore, optimization of the production and thorough functional analyses of the pyomelanin were performed to test its usability in biotechnology. It was confirmed that ANT_H4-derived pyomelanin increases the sun protection factor, enables scavenging of free radicals, and interacts with the iron from minerals. Moreover, it was shown for the first time that pyomelanin exhibits priming properties toward Calendula officinalis hairy roots in in vitro cultures. Conclusions Results of the study indicate the significant biotechnological potential of ANT_H4-derived pyomelanin and open opportunities for future applications. Taking into account protective features of analyzed pyomelanin it may be potentially used in medical biotechnology and cosmetology. Especially interesting was showing that pyomelanin exhibits priming properties toward hairy roots, which creates a perspective for its usage for the development of novel and sustainable agrotechnical solutions. Article in Journal/Newspaper Antarc* Antarctic Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic Microbial Cell Factories 21 1 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctica Biopolymer Pseudomonas Priming of hairy roots Pyomelanin Microbiology QR1-502 |
spellingShingle |
Antarctica Biopolymer Pseudomonas Priming of hairy roots Pyomelanin Microbiology QR1-502 Michal Styczynski Agata Rogowska Christine Nyabayo Przemyslaw Decewicz Filip Romaniuk Cezary Pączkowski Anna Szakiel Roderich Suessmuth Lukasz Dziewit Heterologous production and characterization of a pyomelanin of Antarctic Pseudomonas sp. ANT_H4: a metabolite protecting against UV and free radicals, interacting with iron from minerals and exhibiting priming properties toward plant hairy roots |
topic_facet |
Antarctica Biopolymer Pseudomonas Priming of hairy roots Pyomelanin Microbiology QR1-502 |
description |
Abstract Background Antarctica has one of the most extreme environments in the world. This region is inhabited by specifically adapted microorganisms that produce various unique secondary metabolites (e.g. pigments) enabling their survival under the harsh environmental conditions. It was already shown that these natural, biologically active molecules may find application in various fields of biotechnology. Results In this study, a cold-active brown-pigment-producing Pseudomonas sp. ANT_H4 strain was characterized. In-depth genomic analysis combined with the application of a fosmid expression system revealed two different pathways of melanin-like compounds biosynthesis by the ANT_H4 strain. The chromatographic behavior and Fourier-transform infrared spectroscopic analyses allowed for the identification of the extracted melanin-like compound as a pyomelanin. Furthermore, optimization of the production and thorough functional analyses of the pyomelanin were performed to test its usability in biotechnology. It was confirmed that ANT_H4-derived pyomelanin increases the sun protection factor, enables scavenging of free radicals, and interacts with the iron from minerals. Moreover, it was shown for the first time that pyomelanin exhibits priming properties toward Calendula officinalis hairy roots in in vitro cultures. Conclusions Results of the study indicate the significant biotechnological potential of ANT_H4-derived pyomelanin and open opportunities for future applications. Taking into account protective features of analyzed pyomelanin it may be potentially used in medical biotechnology and cosmetology. Especially interesting was showing that pyomelanin exhibits priming properties toward hairy roots, which creates a perspective for its usage for the development of novel and sustainable agrotechnical solutions. |
format |
Article in Journal/Newspaper |
author |
Michal Styczynski Agata Rogowska Christine Nyabayo Przemyslaw Decewicz Filip Romaniuk Cezary Pączkowski Anna Szakiel Roderich Suessmuth Lukasz Dziewit |
author_facet |
Michal Styczynski Agata Rogowska Christine Nyabayo Przemyslaw Decewicz Filip Romaniuk Cezary Pączkowski Anna Szakiel Roderich Suessmuth Lukasz Dziewit |
author_sort |
Michal Styczynski |
title |
Heterologous production and characterization of a pyomelanin of Antarctic Pseudomonas sp. ANT_H4: a metabolite protecting against UV and free radicals, interacting with iron from minerals and exhibiting priming properties toward plant hairy roots |
title_short |
Heterologous production and characterization of a pyomelanin of Antarctic Pseudomonas sp. ANT_H4: a metabolite protecting against UV and free radicals, interacting with iron from minerals and exhibiting priming properties toward plant hairy roots |
title_full |
Heterologous production and characterization of a pyomelanin of Antarctic Pseudomonas sp. ANT_H4: a metabolite protecting against UV and free radicals, interacting with iron from minerals and exhibiting priming properties toward plant hairy roots |
title_fullStr |
Heterologous production and characterization of a pyomelanin of Antarctic Pseudomonas sp. ANT_H4: a metabolite protecting against UV and free radicals, interacting with iron from minerals and exhibiting priming properties toward plant hairy roots |
title_full_unstemmed |
Heterologous production and characterization of a pyomelanin of Antarctic Pseudomonas sp. ANT_H4: a metabolite protecting against UV and free radicals, interacting with iron from minerals and exhibiting priming properties toward plant hairy roots |
title_sort |
heterologous production and characterization of a pyomelanin of antarctic pseudomonas sp. ant_h4: a metabolite protecting against uv and free radicals, interacting with iron from minerals and exhibiting priming properties toward plant hairy roots |
publisher |
BMC |
publishDate |
2022 |
url |
https://doi.org/10.1186/s12934-022-01990-3 https://doaj.org/article/f13a3b6acef24787b8504e6c037ce058 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Microbial Cell Factories, Vol 21, Iss 1, Pp 1-17 (2022) |
op_relation |
https://doi.org/10.1186/s12934-022-01990-3 https://doaj.org/toc/1475-2859 doi:10.1186/s12934-022-01990-3 1475-2859 https://doaj.org/article/f13a3b6acef24787b8504e6c037ce058 |
op_doi |
https://doi.org/10.1186/s12934-022-01990-3 |
container_title |
Microbial Cell Factories |
container_volume |
21 |
container_issue |
1 |
_version_ |
1766254298329513984 |