Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.)
The aim of this study was to assess the effect of the transfer from freshwater to seawater on the distal intestinal bacterial communities of Atlantic salmon (Salmo salar L.) and to evaluate the effect of dietary inclusion of Pediococcus acidilactici MA18/5M (at 1.19 × 106 CFU/g). In this context, fi...
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Online Access: | https://doi.org/10.3389/fmicb.2019.02243 https://doaj.org/article/f12e02b533624b6db0106cf157b85c37 |
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ftdoajarticles:oai:doaj.org/article:f12e02b533624b6db0106cf157b85c37 2023-05-15T15:31:12+02:00 Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) Alexander Jaramillo-Torres Mark D. Rawling Ana Rodiles Heidi E. Mikalsen Lill-Heidi Johansen John Tinsley Torunn Forberg Elisabeth Aasum Mathieu Castex Daniel Lee Merrifield 2019-09-01T00:00:00Z https://doi.org/10.3389/fmicb.2019.02243 https://doaj.org/article/f12e02b533624b6db0106cf157b85c37 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fmicb.2019.02243/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2019.02243 https://doaj.org/article/f12e02b533624b6db0106cf157b85c37 Frontiers in Microbiology, Vol 10 (2019) fish microbiota intestine Pediococcus acidilactici seawater transfer high-throughput sequencing Microbiology QR1-502 article 2019 ftdoajarticles https://doi.org/10.3389/fmicb.2019.02243 2022-12-31T12:27:31Z The aim of this study was to assess the effect of the transfer from freshwater to seawater on the distal intestinal bacterial communities of Atlantic salmon (Salmo salar L.) and to evaluate the effect of dietary inclusion of Pediococcus acidilactici MA18/5M (at 1.19 × 106 CFU/g). In this context, fish health and antiviral response were also investigated. A 12-week feeding trial was conducted in a flow-through rearing system involving 6 weeks in freshwater and 6 weeks in seawater. Fish received a control and probiotic diet. The composition of the salmon gut bacterial communities was determined by high-throughput sequencing of digesta and mucosa samples from both the freshwater and seawater stage. The main phyla detected during both freshwater and seawater stages were Firmicutes, Proteobacteria, Fusobacteria, and Actinobacteria. Significant differences were observed between the intestinal microbiota in the digesta and the mucosa. Both probiotic supplementation and the seawater transfer (SWT) had a substantial impact on the microbial communities, with most pronounced changes detected in the mucosal communities after SWT. This last finding together with a significantly higher antiviral response (mx-1 and tlr3 gene expression) in the distal intestine of fish fed the probiotic diet suggest a causal link between the microbiota modulation and activation of antiviral response. Feeding probiotics during the freshwater stage did not significantly increase survival after infectious pancreatic necrosis virus (IPNV) challenge after SWT, although higher survival was observed in one out of two replicate challenge tanks. In conclusion, this study demonstrated that both dietary probiotic supplementation and transfer from freshwater to seawater have an important role in modulating the bacterial communities in the distal intestine of Atlantic salmon. Furthermore, supplementation of the diet with P. acidilactici MA18/5M can modulate antiviral response. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Frontiers in Microbiology 10 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
fish microbiota intestine Pediococcus acidilactici seawater transfer high-throughput sequencing Microbiology QR1-502 |
spellingShingle |
fish microbiota intestine Pediococcus acidilactici seawater transfer high-throughput sequencing Microbiology QR1-502 Alexander Jaramillo-Torres Mark D. Rawling Ana Rodiles Heidi E. Mikalsen Lill-Heidi Johansen John Tinsley Torunn Forberg Elisabeth Aasum Mathieu Castex Daniel Lee Merrifield Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) |
topic_facet |
fish microbiota intestine Pediococcus acidilactici seawater transfer high-throughput sequencing Microbiology QR1-502 |
description |
The aim of this study was to assess the effect of the transfer from freshwater to seawater on the distal intestinal bacterial communities of Atlantic salmon (Salmo salar L.) and to evaluate the effect of dietary inclusion of Pediococcus acidilactici MA18/5M (at 1.19 × 106 CFU/g). In this context, fish health and antiviral response were also investigated. A 12-week feeding trial was conducted in a flow-through rearing system involving 6 weeks in freshwater and 6 weeks in seawater. Fish received a control and probiotic diet. The composition of the salmon gut bacterial communities was determined by high-throughput sequencing of digesta and mucosa samples from both the freshwater and seawater stage. The main phyla detected during both freshwater and seawater stages were Firmicutes, Proteobacteria, Fusobacteria, and Actinobacteria. Significant differences were observed between the intestinal microbiota in the digesta and the mucosa. Both probiotic supplementation and the seawater transfer (SWT) had a substantial impact on the microbial communities, with most pronounced changes detected in the mucosal communities after SWT. This last finding together with a significantly higher antiviral response (mx-1 and tlr3 gene expression) in the distal intestine of fish fed the probiotic diet suggest a causal link between the microbiota modulation and activation of antiviral response. Feeding probiotics during the freshwater stage did not significantly increase survival after infectious pancreatic necrosis virus (IPNV) challenge after SWT, although higher survival was observed in one out of two replicate challenge tanks. In conclusion, this study demonstrated that both dietary probiotic supplementation and transfer from freshwater to seawater have an important role in modulating the bacterial communities in the distal intestine of Atlantic salmon. Furthermore, supplementation of the diet with P. acidilactici MA18/5M can modulate antiviral response. |
format |
Article in Journal/Newspaper |
author |
Alexander Jaramillo-Torres Mark D. Rawling Ana Rodiles Heidi E. Mikalsen Lill-Heidi Johansen John Tinsley Torunn Forberg Elisabeth Aasum Mathieu Castex Daniel Lee Merrifield |
author_facet |
Alexander Jaramillo-Torres Mark D. Rawling Ana Rodiles Heidi E. Mikalsen Lill-Heidi Johansen John Tinsley Torunn Forberg Elisabeth Aasum Mathieu Castex Daniel Lee Merrifield |
author_sort |
Alexander Jaramillo-Torres |
title |
Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) |
title_short |
Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) |
title_full |
Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) |
title_fullStr |
Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) |
title_full_unstemmed |
Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) |
title_sort |
influence of dietary supplementation of probiotic pediococcus acidilactici ma18/5m during the transition from freshwater to seawater on intestinal health and microbiota of atlantic salmon (salmo salar l.) |
publisher |
Frontiers Media S.A. |
publishDate |
2019 |
url |
https://doi.org/10.3389/fmicb.2019.02243 https://doaj.org/article/f12e02b533624b6db0106cf157b85c37 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Frontiers in Microbiology, Vol 10 (2019) |
op_relation |
https://www.frontiersin.org/article/10.3389/fmicb.2019.02243/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2019.02243 https://doaj.org/article/f12e02b533624b6db0106cf157b85c37 |
op_doi |
https://doi.org/10.3389/fmicb.2019.02243 |
container_title |
Frontiers in Microbiology |
container_volume |
10 |
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1766361701533351936 |