Sex-biased polyparasitism in moose (Alces alces) based on molecular analysis of faecal samples
Simultaneous infection with multiple parasite species in an individual host is often observed in wild populations. The understanding of parasite species distribution across populations of wild animals is of basic and applied importance, because parasites can have pronounced effects on the dynamics o...
Published in: | International Journal for Parasitology: Parasites and Wildlife |
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Online Access: | https://doi.org/10.1016/j.ijppaw.2020.10.008 https://doaj.org/article/f0de4c6ff1f443fd90ef1af7344ec456 |
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ftdoajarticles:oai:doaj.org/article:f0de4c6ff1f443fd90ef1af7344ec456 2023-05-15T13:13:41+02:00 Sex-biased polyparasitism in moose (Alces alces) based on molecular analysis of faecal samples Magdalena Świsłocka Anetta Borkowska Maciej Matosiuk Magdalena Czajkowska Norbert Duda Rafał Kowalczyk Mirosław Ratkiewicz 2020-12-01T00:00:00Z https://doi.org/10.1016/j.ijppaw.2020.10.008 https://doaj.org/article/f0de4c6ff1f443fd90ef1af7344ec456 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2213224420300985 https://doaj.org/toc/2213-2244 2213-2244 doi:10.1016/j.ijppaw.2020.10.008 https://doaj.org/article/f0de4c6ff1f443fd90ef1af7344ec456 International Journal for Parasitology: Parasites and Wildlife, Vol 13, Iss , Pp 171-177 (2020) Endoparasite richness Liver fluke Nematodes Sex-biased parasitism Tapeworms Zoology QL1-991 article 2020 ftdoajarticles https://doi.org/10.1016/j.ijppaw.2020.10.008 2022-12-31T04:56:17Z Simultaneous infection with multiple parasite species in an individual host is often observed in wild populations. The understanding of parasite species distribution across populations of wild animals is of basic and applied importance, because parasites can have pronounced effects on the dynamics of host population. Here, we quantified prevalence and endoparasite species richness in moose and explored sex-biased polyparasitism using diagnostic PCR method coupled with DNA sequencing of moose faecal samples from the Biebrza River valley, North-Eastern Poland. This is the largest moose population in Central Europe that has not been harvested for almost 20 years. We also evaluated the appropriate quantity of faeces for detecting DNA of parasite species. Faecal samples were screened for molecular markers of 10 different species of endoparasites. Endoparasite prevalence was high in the studied population. Almost all of the samples (98%) tested positive for at least one parasite species, and we found polyparasitism in the majority of the tested individuals. The number of different parasite species found in a single individual ranged from 0 to 9. The parasite species richness was significantly higher in male than in female individuals. The most prevalent were liver fluke Parafasciolopsis fasciolaemorpha and gastrointestinal nematodes Ostertargia sp. Of the ten endoparasite species detected, only the prevalence of the tapeworm Moniezia benedeni was significantly higher in males than in females. Additionally, we identified co-occurrence associations of parasite species, which tended to be random, but we noted some evidence of both positive and negative associations. Our findings promote applications of molecular methods for parasite species identification from non-invasively collected faecal samples in management and scientific study of moose population, which should include investigation of parasite status, and in health monitoring programs for other wild cervids. Article in Journal/Newspaper Alces alces Directory of Open Access Journals: DOAJ Articles International Journal for Parasitology: Parasites and Wildlife 13 171 177 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Endoparasite richness Liver fluke Nematodes Sex-biased parasitism Tapeworms Zoology QL1-991 |
spellingShingle |
Endoparasite richness Liver fluke Nematodes Sex-biased parasitism Tapeworms Zoology QL1-991 Magdalena Świsłocka Anetta Borkowska Maciej Matosiuk Magdalena Czajkowska Norbert Duda Rafał Kowalczyk Mirosław Ratkiewicz Sex-biased polyparasitism in moose (Alces alces) based on molecular analysis of faecal samples |
topic_facet |
Endoparasite richness Liver fluke Nematodes Sex-biased parasitism Tapeworms Zoology QL1-991 |
description |
Simultaneous infection with multiple parasite species in an individual host is often observed in wild populations. The understanding of parasite species distribution across populations of wild animals is of basic and applied importance, because parasites can have pronounced effects on the dynamics of host population. Here, we quantified prevalence and endoparasite species richness in moose and explored sex-biased polyparasitism using diagnostic PCR method coupled with DNA sequencing of moose faecal samples from the Biebrza River valley, North-Eastern Poland. This is the largest moose population in Central Europe that has not been harvested for almost 20 years. We also evaluated the appropriate quantity of faeces for detecting DNA of parasite species. Faecal samples were screened for molecular markers of 10 different species of endoparasites. Endoparasite prevalence was high in the studied population. Almost all of the samples (98%) tested positive for at least one parasite species, and we found polyparasitism in the majority of the tested individuals. The number of different parasite species found in a single individual ranged from 0 to 9. The parasite species richness was significantly higher in male than in female individuals. The most prevalent were liver fluke Parafasciolopsis fasciolaemorpha and gastrointestinal nematodes Ostertargia sp. Of the ten endoparasite species detected, only the prevalence of the tapeworm Moniezia benedeni was significantly higher in males than in females. Additionally, we identified co-occurrence associations of parasite species, which tended to be random, but we noted some evidence of both positive and negative associations. Our findings promote applications of molecular methods for parasite species identification from non-invasively collected faecal samples in management and scientific study of moose population, which should include investigation of parasite status, and in health monitoring programs for other wild cervids. |
format |
Article in Journal/Newspaper |
author |
Magdalena Świsłocka Anetta Borkowska Maciej Matosiuk Magdalena Czajkowska Norbert Duda Rafał Kowalczyk Mirosław Ratkiewicz |
author_facet |
Magdalena Świsłocka Anetta Borkowska Maciej Matosiuk Magdalena Czajkowska Norbert Duda Rafał Kowalczyk Mirosław Ratkiewicz |
author_sort |
Magdalena Świsłocka |
title |
Sex-biased polyparasitism in moose (Alces alces) based on molecular analysis of faecal samples |
title_short |
Sex-biased polyparasitism in moose (Alces alces) based on molecular analysis of faecal samples |
title_full |
Sex-biased polyparasitism in moose (Alces alces) based on molecular analysis of faecal samples |
title_fullStr |
Sex-biased polyparasitism in moose (Alces alces) based on molecular analysis of faecal samples |
title_full_unstemmed |
Sex-biased polyparasitism in moose (Alces alces) based on molecular analysis of faecal samples |
title_sort |
sex-biased polyparasitism in moose (alces alces) based on molecular analysis of faecal samples |
publisher |
Elsevier |
publishDate |
2020 |
url |
https://doi.org/10.1016/j.ijppaw.2020.10.008 https://doaj.org/article/f0de4c6ff1f443fd90ef1af7344ec456 |
genre |
Alces alces |
genre_facet |
Alces alces |
op_source |
International Journal for Parasitology: Parasites and Wildlife, Vol 13, Iss , Pp 171-177 (2020) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2213224420300985 https://doaj.org/toc/2213-2244 2213-2244 doi:10.1016/j.ijppaw.2020.10.008 https://doaj.org/article/f0de4c6ff1f443fd90ef1af7344ec456 |
op_doi |
https://doi.org/10.1016/j.ijppaw.2020.10.008 |
container_title |
International Journal for Parasitology: Parasites and Wildlife |
container_volume |
13 |
container_start_page |
171 |
op_container_end_page |
177 |
_version_ |
1766259853814136832 |