Cryptic Zooplankton Diversity Revealed by a Metagenetic Approach to Monitoring Metazoan Communities in the Coastal Waters of the Okhotsk Sea, Northeastern Hokkaido
Monitoring zooplankton communities is important to understand dynamics in marine ecosystems. However, it is difficult to identify cryptic species and immature stages of zooplankton using morphological classification, which is time-consuming and requires high skill levels. Here, we conducted a metage...
Published in: | Frontiers in Marine Science |
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ftdoajarticles:oai:doaj.org/article:f0b93d312db445fcb6bb5088859210c8 2023-05-15T17:52:38+02:00 Cryptic Zooplankton Diversity Revealed by a Metagenetic Approach to Monitoring Metazoan Communities in the Coastal Waters of the Okhotsk Sea, Northeastern Hokkaido Junya Hirai Seiji Katakura Hiromi Kasai Satoshi Nagai 2017-12-01T00:00:00Z https://doi.org/10.3389/fmars.2017.00379 https://doaj.org/article/f0b93d312db445fcb6bb5088859210c8 EN eng Frontiers Media S.A. http://journal.frontiersin.org/article/10.3389/fmars.2017.00379/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2017.00379 https://doaj.org/article/f0b93d312db445fcb6bb5088859210c8 Frontiers in Marine Science, Vol 4 (2017) zooplankton metagenetics diversity coastal waters Okhotsk Sea Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2017 ftdoajarticles https://doi.org/10.3389/fmars.2017.00379 2023-01-08T01:31:56Z Monitoring zooplankton communities is important to understand dynamics in marine ecosystems. However, it is difficult to identify cryptic species and immature stages of zooplankton using morphological classification, which is time-consuming and requires high skill levels. Here, we conducted a metagenetic analysis of the 18S region in 101 zooplankton samples collected weekly throughout 2014 and 2015 at the Okhotsk Tower in Mombetsu, Hokkaido, Japan, and compared the results of this analysis with those provided by morphological analysis. The metagenetic analysis detected 561 molecular taxonomic units (MOTUs), whereas the morphological analysis detected 201 taxonomic groups. Zooplankton communities were dominated by copepods throughout the sampling period; however, non-copepod taxa, which comprised high proportions of both MOTUs (mean 51.1%) and sequence reads (mean 19.1%), were also important. Cryptic diversity detected by the metagenetic analysis was primarily driven by Copepoda and by the larvae of benthic taxa such as Bivalvia, Gastropoda, and Polychaeta. Community structure and diversity varied between periods of warm and cold water, indicating strong correlations with water temperature and thus seasonality. Furthermore, metagenetic analysis revealed detailed seasonal changes in dominant taxa, including larval stages of metazoans with high taxonomic resolutions; these included commercially important organisms such as Japanese scallops. The metagenetic analysis revealed that changes in both water mass and bentho-pelagic interactions sustain ecosystems rich in zooplankton diversity in this area. Metagenetic analysis provides novel insight into zooplankton diversity, and generates massive sequence data that may be used in future research; thus, it is considered an effective tool for monitoring zooplankton communities. Article in Journal/Newspaper okhotsk sea Copepods Directory of Open Access Journals: DOAJ Articles Okhotsk Frontiers in Marine Science 4 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
zooplankton metagenetics diversity coastal waters Okhotsk Sea Science Q General. Including nature conservation geographical distribution QH1-199.5 |
spellingShingle |
zooplankton metagenetics diversity coastal waters Okhotsk Sea Science Q General. Including nature conservation geographical distribution QH1-199.5 Junya Hirai Seiji Katakura Hiromi Kasai Satoshi Nagai Cryptic Zooplankton Diversity Revealed by a Metagenetic Approach to Monitoring Metazoan Communities in the Coastal Waters of the Okhotsk Sea, Northeastern Hokkaido |
topic_facet |
zooplankton metagenetics diversity coastal waters Okhotsk Sea Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
Monitoring zooplankton communities is important to understand dynamics in marine ecosystems. However, it is difficult to identify cryptic species and immature stages of zooplankton using morphological classification, which is time-consuming and requires high skill levels. Here, we conducted a metagenetic analysis of the 18S region in 101 zooplankton samples collected weekly throughout 2014 and 2015 at the Okhotsk Tower in Mombetsu, Hokkaido, Japan, and compared the results of this analysis with those provided by morphological analysis. The metagenetic analysis detected 561 molecular taxonomic units (MOTUs), whereas the morphological analysis detected 201 taxonomic groups. Zooplankton communities were dominated by copepods throughout the sampling period; however, non-copepod taxa, which comprised high proportions of both MOTUs (mean 51.1%) and sequence reads (mean 19.1%), were also important. Cryptic diversity detected by the metagenetic analysis was primarily driven by Copepoda and by the larvae of benthic taxa such as Bivalvia, Gastropoda, and Polychaeta. Community structure and diversity varied between periods of warm and cold water, indicating strong correlations with water temperature and thus seasonality. Furthermore, metagenetic analysis revealed detailed seasonal changes in dominant taxa, including larval stages of metazoans with high taxonomic resolutions; these included commercially important organisms such as Japanese scallops. The metagenetic analysis revealed that changes in both water mass and bentho-pelagic interactions sustain ecosystems rich in zooplankton diversity in this area. Metagenetic analysis provides novel insight into zooplankton diversity, and generates massive sequence data that may be used in future research; thus, it is considered an effective tool for monitoring zooplankton communities. |
format |
Article in Journal/Newspaper |
author |
Junya Hirai Seiji Katakura Hiromi Kasai Satoshi Nagai |
author_facet |
Junya Hirai Seiji Katakura Hiromi Kasai Satoshi Nagai |
author_sort |
Junya Hirai |
title |
Cryptic Zooplankton Diversity Revealed by a Metagenetic Approach to Monitoring Metazoan Communities in the Coastal Waters of the Okhotsk Sea, Northeastern Hokkaido |
title_short |
Cryptic Zooplankton Diversity Revealed by a Metagenetic Approach to Monitoring Metazoan Communities in the Coastal Waters of the Okhotsk Sea, Northeastern Hokkaido |
title_full |
Cryptic Zooplankton Diversity Revealed by a Metagenetic Approach to Monitoring Metazoan Communities in the Coastal Waters of the Okhotsk Sea, Northeastern Hokkaido |
title_fullStr |
Cryptic Zooplankton Diversity Revealed by a Metagenetic Approach to Monitoring Metazoan Communities in the Coastal Waters of the Okhotsk Sea, Northeastern Hokkaido |
title_full_unstemmed |
Cryptic Zooplankton Diversity Revealed by a Metagenetic Approach to Monitoring Metazoan Communities in the Coastal Waters of the Okhotsk Sea, Northeastern Hokkaido |
title_sort |
cryptic zooplankton diversity revealed by a metagenetic approach to monitoring metazoan communities in the coastal waters of the okhotsk sea, northeastern hokkaido |
publisher |
Frontiers Media S.A. |
publishDate |
2017 |
url |
https://doi.org/10.3389/fmars.2017.00379 https://doaj.org/article/f0b93d312db445fcb6bb5088859210c8 |
geographic |
Okhotsk |
geographic_facet |
Okhotsk |
genre |
okhotsk sea Copepods |
genre_facet |
okhotsk sea Copepods |
op_source |
Frontiers in Marine Science, Vol 4 (2017) |
op_relation |
http://journal.frontiersin.org/article/10.3389/fmars.2017.00379/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2017.00379 https://doaj.org/article/f0b93d312db445fcb6bb5088859210c8 |
op_doi |
https://doi.org/10.3389/fmars.2017.00379 |
container_title |
Frontiers in Marine Science |
container_volume |
4 |
_version_ |
1766160323484581888 |