Molecular characterization of Cryptococcus neoformans isolates from HIV patients, Guayaquil, Ecuador
Introduction: Neurocryptococcosis is an opportunistic fungal infection that represents a high cost in human lives and for the economy of countries. Its causative agent, the Cryptococcus neoformans/Cryptococcus gattii species complex, has a sexual and an asexual phase, four major serotypes and seven...
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ftdoajarticles:oai:doaj.org/article:f09bed3a838142c98cdea1b4f8e1fa6c 2023-05-15T15:06:19+02:00 Molecular characterization of Cryptococcus neoformans isolates from HIV patients, Guayaquil, Ecuador Sunny Sánchez Dolores Zambrano Maylen García César Bedoya Carlos Fernández María Teresa Illnait-Zaragozí 2017-09-01T00:00:00Z https://doi.org/10.7705/biomedica.v37i3.3322 https://doaj.org/article/f09bed3a838142c98cdea1b4f8e1fa6c EN ES eng spa Instituto Nacional de Salud https://www.revistabiomedica.org/index.php/biomedica/article/view/3322 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v37i3.3322 https://doaj.org/article/f09bed3a838142c98cdea1b4f8e1fa6c Biomédica: revista del Instituto Nacional de Salud, Vol 37, Iss 3, Pp 425-430 (2017) Cryptococcus neoformans cryptococcosis HIV acquired immunodeficiency syndrome genotype Ecuador Medicine R Arctic medicine. Tropical medicine RC955-962 article 2017 ftdoajarticles https://doi.org/10.7705/biomedica.v37i3.3322 2022-12-30T23:55:59Z Introduction: Neurocryptococcosis is an opportunistic fungal infection that represents a high cost in human lives and for the economy of countries. Its causative agent, the Cryptococcus neoformans/Cryptococcus gattii species complex, has a sexual and an asexual phase, four major serotypes and seven molecular varieties with phenotypic, clinical-epidemiological and antifungal susceptibility differences. Objective: To characterize by molecular methods clinical isolates of C. neoformans from Guayaquil, Ecuador. Materials and methods: We determined mating types, serotypes and molecular varieties by PCR and RFLP in 27 yeast isolates previously identified as C. neoformans by conventional methods. The isolates were recovered from cerebrospinal fluid of HIV seropositive patients with neurological syndrome admitted at “Dr. José Daniel Rodríguez Maridueña” Hospital from December, 2013, to January, 2015. Results: We established a wide prevalence of C. neoformans serotype A, MATα and genotype VNI among the studied isolates. Conclusions: These data are similar to those obtained in other countries and the first identified by molecular characterization in Guayaquil, Ecuador. Therefore, they constitute an important contribution to the knowledge on cryptococcosis in this country. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Guayaquil ENVELOPE(-59.767,-59.767,-62.449,-62.449) Biomédica 37 3 425 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English Spanish |
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Cryptococcus neoformans cryptococcosis HIV acquired immunodeficiency syndrome genotype Ecuador Medicine R Arctic medicine. Tropical medicine RC955-962 |
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Cryptococcus neoformans cryptococcosis HIV acquired immunodeficiency syndrome genotype Ecuador Medicine R Arctic medicine. Tropical medicine RC955-962 Sunny Sánchez Dolores Zambrano Maylen García César Bedoya Carlos Fernández María Teresa Illnait-Zaragozí Molecular characterization of Cryptococcus neoformans isolates from HIV patients, Guayaquil, Ecuador |
topic_facet |
Cryptococcus neoformans cryptococcosis HIV acquired immunodeficiency syndrome genotype Ecuador Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: Neurocryptococcosis is an opportunistic fungal infection that represents a high cost in human lives and for the economy of countries. Its causative agent, the Cryptococcus neoformans/Cryptococcus gattii species complex, has a sexual and an asexual phase, four major serotypes and seven molecular varieties with phenotypic, clinical-epidemiological and antifungal susceptibility differences. Objective: To characterize by molecular methods clinical isolates of C. neoformans from Guayaquil, Ecuador. Materials and methods: We determined mating types, serotypes and molecular varieties by PCR and RFLP in 27 yeast isolates previously identified as C. neoformans by conventional methods. The isolates were recovered from cerebrospinal fluid of HIV seropositive patients with neurological syndrome admitted at “Dr. José Daniel Rodríguez Maridueña” Hospital from December, 2013, to January, 2015. Results: We established a wide prevalence of C. neoformans serotype A, MATα and genotype VNI among the studied isolates. Conclusions: These data are similar to those obtained in other countries and the first identified by molecular characterization in Guayaquil, Ecuador. Therefore, they constitute an important contribution to the knowledge on cryptococcosis in this country. |
format |
Article in Journal/Newspaper |
author |
Sunny Sánchez Dolores Zambrano Maylen García César Bedoya Carlos Fernández María Teresa Illnait-Zaragozí |
author_facet |
Sunny Sánchez Dolores Zambrano Maylen García César Bedoya Carlos Fernández María Teresa Illnait-Zaragozí |
author_sort |
Sunny Sánchez |
title |
Molecular characterization of Cryptococcus neoformans isolates from HIV patients, Guayaquil, Ecuador |
title_short |
Molecular characterization of Cryptococcus neoformans isolates from HIV patients, Guayaquil, Ecuador |
title_full |
Molecular characterization of Cryptococcus neoformans isolates from HIV patients, Guayaquil, Ecuador |
title_fullStr |
Molecular characterization of Cryptococcus neoformans isolates from HIV patients, Guayaquil, Ecuador |
title_full_unstemmed |
Molecular characterization of Cryptococcus neoformans isolates from HIV patients, Guayaquil, Ecuador |
title_sort |
molecular characterization of cryptococcus neoformans isolates from hiv patients, guayaquil, ecuador |
publisher |
Instituto Nacional de Salud |
publishDate |
2017 |
url |
https://doi.org/10.7705/biomedica.v37i3.3322 https://doaj.org/article/f09bed3a838142c98cdea1b4f8e1fa6c |
long_lat |
ENVELOPE(-59.767,-59.767,-62.449,-62.449) |
geographic |
Arctic Guayaquil |
geographic_facet |
Arctic Guayaquil |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 37, Iss 3, Pp 425-430 (2017) |
op_relation |
https://www.revistabiomedica.org/index.php/biomedica/article/view/3322 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v37i3.3322 https://doaj.org/article/f09bed3a838142c98cdea1b4f8e1fa6c |
op_doi |
https://doi.org/10.7705/biomedica.v37i3.3322 |
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Biomédica |
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37 |
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425 |
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