Transcriptomic and functional analyses of the piRNA pathway in the Chagas disease vector Rhodnius prolixus.
The piRNA pathway is a surveillance system that guarantees oogenesis and adult fertility in a range of animal species. The pathway is centered on PIWI clade Argonaute proteins and the associated small non-coding RNAs termed piRNAs. In this study, we set to investigate the evolutionary conservation o...
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ftdoajarticles:oai:doaj.org/article:f0655a1f157c40bc81b1de2e03a61ca3 2023-05-15T15:10:40+02:00 Transcriptomic and functional analyses of the piRNA pathway in the Chagas disease vector Rhodnius prolixus. Tarcisio Brito Alison Julio Mateus Berni Lisiane de Castro Poncio Emerson Soares Bernardes Helena Araujo Michael Sammeth Attilio Pane 2018-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006760 https://doaj.org/article/f0655a1f157c40bc81b1de2e03a61ca3 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6179187?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006760 https://doaj.org/article/f0655a1f157c40bc81b1de2e03a61ca3 PLoS Neglected Tropical Diseases, Vol 12, Iss 10, p e0006760 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006760 2022-12-31T14:37:39Z The piRNA pathway is a surveillance system that guarantees oogenesis and adult fertility in a range of animal species. The pathway is centered on PIWI clade Argonaute proteins and the associated small non-coding RNAs termed piRNAs. In this study, we set to investigate the evolutionary conservation of the piRNA pathway in the hemimetabolous insect Rhodnius prolixus. Our transcriptome profiling reveals that core components of the pathway are expressed during previtellogenic stages of oogenesis. Rhodnius' genome harbors four putative piwi orthologs. We show that Rp-piwi2, Rp-piwi3 and Rp-ago3, but not Rp-piwi1 transcripts are produced in the germline tissues and maternally deposited in the mature eggs. Consistent with a role in Rhodnius oogenesis, parental RNAi against the Rp-piwi2, Rp-piwi3 and Rp-ago3 results in severe egg laying and female adult fertility defects. Furthermore, we show that the reduction of the Rp-piwi2 levels by parental RNAi disrupts oogenesis by causing a dramatic loss of trophocytes, egg chamber degeneration and oogenesis arrest. Intriguingly, the putative Rp-Piwi2 protein features a polyglutamine tract at its N-terminal region, which is conserved in PIWI proteins encoded in the genome of other Triatomine species. Together with R. prolixus, these hematophagous insects are primary vectors of the Chagas disease. Thus, our data shed more light on the evolution of the piRNA pathway and provide a framework for the development of new control strategies for Chagas disease insect vectors. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 10 e0006760 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Tarcisio Brito Alison Julio Mateus Berni Lisiane de Castro Poncio Emerson Soares Bernardes Helena Araujo Michael Sammeth Attilio Pane Transcriptomic and functional analyses of the piRNA pathway in the Chagas disease vector Rhodnius prolixus. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The piRNA pathway is a surveillance system that guarantees oogenesis and adult fertility in a range of animal species. The pathway is centered on PIWI clade Argonaute proteins and the associated small non-coding RNAs termed piRNAs. In this study, we set to investigate the evolutionary conservation of the piRNA pathway in the hemimetabolous insect Rhodnius prolixus. Our transcriptome profiling reveals that core components of the pathway are expressed during previtellogenic stages of oogenesis. Rhodnius' genome harbors four putative piwi orthologs. We show that Rp-piwi2, Rp-piwi3 and Rp-ago3, but not Rp-piwi1 transcripts are produced in the germline tissues and maternally deposited in the mature eggs. Consistent with a role in Rhodnius oogenesis, parental RNAi against the Rp-piwi2, Rp-piwi3 and Rp-ago3 results in severe egg laying and female adult fertility defects. Furthermore, we show that the reduction of the Rp-piwi2 levels by parental RNAi disrupts oogenesis by causing a dramatic loss of trophocytes, egg chamber degeneration and oogenesis arrest. Intriguingly, the putative Rp-Piwi2 protein features a polyglutamine tract at its N-terminal region, which is conserved in PIWI proteins encoded in the genome of other Triatomine species. Together with R. prolixus, these hematophagous insects are primary vectors of the Chagas disease. Thus, our data shed more light on the evolution of the piRNA pathway and provide a framework for the development of new control strategies for Chagas disease insect vectors. |
format |
Article in Journal/Newspaper |
author |
Tarcisio Brito Alison Julio Mateus Berni Lisiane de Castro Poncio Emerson Soares Bernardes Helena Araujo Michael Sammeth Attilio Pane |
author_facet |
Tarcisio Brito Alison Julio Mateus Berni Lisiane de Castro Poncio Emerson Soares Bernardes Helena Araujo Michael Sammeth Attilio Pane |
author_sort |
Tarcisio Brito |
title |
Transcriptomic and functional analyses of the piRNA pathway in the Chagas disease vector Rhodnius prolixus. |
title_short |
Transcriptomic and functional analyses of the piRNA pathway in the Chagas disease vector Rhodnius prolixus. |
title_full |
Transcriptomic and functional analyses of the piRNA pathway in the Chagas disease vector Rhodnius prolixus. |
title_fullStr |
Transcriptomic and functional analyses of the piRNA pathway in the Chagas disease vector Rhodnius prolixus. |
title_full_unstemmed |
Transcriptomic and functional analyses of the piRNA pathway in the Chagas disease vector Rhodnius prolixus. |
title_sort |
transcriptomic and functional analyses of the pirna pathway in the chagas disease vector rhodnius prolixus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006760 https://doaj.org/article/f0655a1f157c40bc81b1de2e03a61ca3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 10, p e0006760 (2018) |
op_relation |
http://europepmc.org/articles/PMC6179187?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006760 https://doaj.org/article/f0655a1f157c40bc81b1de2e03a61ca3 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006760 |
container_title |
PLOS Neglected Tropical Diseases |
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12 |
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10 |
container_start_page |
e0006760 |
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1766341652298858496 |