Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen
Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; in the second (COMP-2), test sera and anti-HA VHRP conjugate competed for HAV epitopes previo...
Published in: | Revista da Sociedade Brasileira de Medicina Tropical |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Sociedade Brasileira de Medicina Tropical (SBMT)
1991
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Online Access: | https://doi.org/10.1590/S0037-86821991000200003 https://doaj.org/article/f0528b9c21354377b298a727ba53fc95 |
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ftdoajarticles:oai:doaj.org/article:f0528b9c21354377b298a727ba53fc95 2023-05-15T14:58:51+02:00 Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen Claudia Lamarca Vitral Ana Maria Coimbra Gaspar Clara Fumiko Tachibana Yoshida 1991-06-01T00:00:00Z https://doi.org/10.1590/S0037-86821991000200003 https://doaj.org/article/f0528b9c21354377b298a727ba53fc95 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821991000200003&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86821991000200003 https://doaj.org/article/f0528b9c21354377b298a727ba53fc95 Revista da Sociedade Brasileira de Medicina Tropical, Vol 24, Iss 2, Pp 79-85 (1991) Enzyme immmunoassay Anti-HAV Hepatitis A Arctic medicine. Tropical medicine RC955-962 article 1991 ftdoajarticles https://doi.org/10.1590/S0037-86821991000200003 2022-12-31T03:01:13Z Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; in the second (COMP-2), test sera and anti-HA VHRP conjugate competed for HAV epitopes previously adsorbed to anti-HA V IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. Both competitive tests were shown to be reproducible and suitable for routine diagnosis and research purposes. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Eia ENVELOPE(7.755,7.755,63.024,63.024) Revista da Sociedade Brasileira de Medicina Tropical 24 2 79 85 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Enzyme immmunoassay Anti-HAV Hepatitis A Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Enzyme immmunoassay Anti-HAV Hepatitis A Arctic medicine. Tropical medicine RC955-962 Claudia Lamarca Vitral Ana Maria Coimbra Gaspar Clara Fumiko Tachibana Yoshida Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen |
topic_facet |
Enzyme immmunoassay Anti-HAV Hepatitis A Arctic medicine. Tropical medicine RC955-962 |
description |
Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; in the second (COMP-2), test sera and anti-HA VHRP conjugate competed for HAV epitopes previously adsorbed to anti-HA V IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. Both competitive tests were shown to be reproducible and suitable for routine diagnosis and research purposes. |
format |
Article in Journal/Newspaper |
author |
Claudia Lamarca Vitral Ana Maria Coimbra Gaspar Clara Fumiko Tachibana Yoshida |
author_facet |
Claudia Lamarca Vitral Ana Maria Coimbra Gaspar Clara Fumiko Tachibana Yoshida |
author_sort |
Claudia Lamarca Vitral |
title |
Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen |
title_short |
Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen |
title_full |
Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen |
title_fullStr |
Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen |
title_full_unstemmed |
Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen |
title_sort |
two competitive enzyme immunoassays for the detection of igg class antibodies to hepatitis a antigen |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
1991 |
url |
https://doi.org/10.1590/S0037-86821991000200003 https://doaj.org/article/f0528b9c21354377b298a727ba53fc95 |
long_lat |
ENVELOPE(7.755,7.755,63.024,63.024) |
geographic |
Arctic Eia |
geographic_facet |
Arctic Eia |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 24, Iss 2, Pp 79-85 (1991) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821991000200003&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86821991000200003 https://doaj.org/article/f0528b9c21354377b298a727ba53fc95 |
op_doi |
https://doi.org/10.1590/S0037-86821991000200003 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
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24 |
container_issue |
2 |
container_start_page |
79 |
op_container_end_page |
85 |
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1766330977206927360 |