Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen

• Published in: Revista da Sociedade Brasileira de Medicina Tropical
• Main Authors: Claudia Lamarca Vitral, Ana Maria Coimbra Gaspar, Clara Fumiko Tachibana Yoshida
• Format: Article in Journal/Newspaper
• Language: English
• Published: Sociedade Brasileira de Medicina Tropical (SBMT) 1991
• Subjects: Enzyme immmunoassay; Anti-HAV; Hepatitis A; Arctic medicine. Tropical medicine; RC955-962; Arctic; Eia
• Online Access: https://doi.org/10.1590/S0037-86821991000200003; https://doaj.org/article/f0528b9c21354377b298a727ba53fc95

Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; in the second (COMP-2), test sera and anti-HA VHRP conjugate competed for HAV epitopes previously adsorbed to anti-HA V IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. Both competitive tests were shown to be reproducible and suitable for routine diagnosis and research purposes.