Immunogenicity analysis of conserved fragments in Plasmodium ovale species merozoite surface protein 4
Abstract Background There is an urgent need for an effective vaccine to control and eradicate malaria, one of the most serious global infectious diseases. Plasmodium merozoite surface protein 4 (MSP4) has been listed as a blood-stage subunit vaccine candidate for malaria. Infection with Plasmodium o...
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ftdoajarticles:oai:doaj.org/article:efdaba21564347b4903ea184c35a940e 2023-05-15T15:15:54+02:00 Immunogenicity analysis of conserved fragments in Plasmodium ovale species merozoite surface protein 4 Juliette Uwase Ruilin Chu Kokouvi Kassegne Yao Lei Feihu Shen Haitian Fu Yifan Sun Yinghua Xuan Jun Cao Yang Cheng 2020-03-01T00:00:00Z https://doi.org/10.1186/s12936-020-03207-7 https://doaj.org/article/efdaba21564347b4903ea184c35a940e EN eng BMC http://link.springer.com/article/10.1186/s12936-020-03207-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03207-7 1475-2875 https://doaj.org/article/efdaba21564347b4903ea184c35a940e Malaria Journal, Vol 19, Iss 1, Pp 1-11 (2020) Plasmodium ovale species MSP4 Conservation Immunogenicity Cross-reactivity Avidity index Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-03207-7 2022-12-31T14:16:05Z Abstract Background There is an urgent need for an effective vaccine to control and eradicate malaria, one of the most serious global infectious diseases. Plasmodium merozoite surface protein 4 (MSP4) has been listed as a blood-stage subunit vaccine candidate for malaria. Infection with Plasmodium ovale species including P. ovale wallikeri and P. ovale curtisi, is also a source of malaria burden in tropical regions where it is sometimes mixed with other Plasmodium species. However, little is known about P. ovale MSP4. Methods The msp4 gene was amplified through polymerase chain reaction using genomic DNA extracted from blood samples of 46 patients infected with P. ovale spp. and amplified products were sequenced. Open reading frames predicted as immunogenic peptides consisting of 119 and 97 amino acids of P. ovale curtisi MSP4 (PocMSP4) and P. ovale wallikeri MSP4 (PowMSP4), respectively, were selected for protein expression. Recombinant proteins (rPoMSP4) were expressed in Escherichia coli, purified, analysed, and immunized in BALB/c mice. The specificity of anti-MSP4-immunoglobulin (Ig) G antibodies was evaluated by Western blot and enzyme-linked immunosorbent assays, and cellular immune responses were analysed via lymphocyte proliferation assays. Results Full peptide sequences of PocMSP4 and PowMSP4 were completely conserved in all clinical isolates, except in the epidermal growth factor-like domain at the carboxyl terminus where only one mutation was observed in one P. o. wallikeri isolate. Further, truncated PoMSP4 segments were successfully expressed and purified as ~ 32 kDa proteins. Importantly, high antibody responses with end-point titres ranging from 1:10,000 to 1:2,560,000 in all immunized mouse groups were observed, with high IgG avidity to PocMSP4 (80.5%) and PowMSP4 (92.3%). Furthermore, rPocMSP4 and rPowMSP4 cross-reacted with anti-PowMSP4-specific or anti-PocMSP4-specific antibodies. Additionally, anti-PoMSP4 IgG antibodies showed broad immuno-specificity in reacting against rPoMSP1 and rPoAMA1. ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Plasmodium ovale species MSP4 Conservation Immunogenicity Cross-reactivity Avidity index Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Plasmodium ovale species MSP4 Conservation Immunogenicity Cross-reactivity Avidity index Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Juliette Uwase Ruilin Chu Kokouvi Kassegne Yao Lei Feihu Shen Haitian Fu Yifan Sun Yinghua Xuan Jun Cao Yang Cheng Immunogenicity analysis of conserved fragments in Plasmodium ovale species merozoite surface protein 4 |
topic_facet |
Plasmodium ovale species MSP4 Conservation Immunogenicity Cross-reactivity Avidity index Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background There is an urgent need for an effective vaccine to control and eradicate malaria, one of the most serious global infectious diseases. Plasmodium merozoite surface protein 4 (MSP4) has been listed as a blood-stage subunit vaccine candidate for malaria. Infection with Plasmodium ovale species including P. ovale wallikeri and P. ovale curtisi, is also a source of malaria burden in tropical regions where it is sometimes mixed with other Plasmodium species. However, little is known about P. ovale MSP4. Methods The msp4 gene was amplified through polymerase chain reaction using genomic DNA extracted from blood samples of 46 patients infected with P. ovale spp. and amplified products were sequenced. Open reading frames predicted as immunogenic peptides consisting of 119 and 97 amino acids of P. ovale curtisi MSP4 (PocMSP4) and P. ovale wallikeri MSP4 (PowMSP4), respectively, were selected for protein expression. Recombinant proteins (rPoMSP4) were expressed in Escherichia coli, purified, analysed, and immunized in BALB/c mice. The specificity of anti-MSP4-immunoglobulin (Ig) G antibodies was evaluated by Western blot and enzyme-linked immunosorbent assays, and cellular immune responses were analysed via lymphocyte proliferation assays. Results Full peptide sequences of PocMSP4 and PowMSP4 were completely conserved in all clinical isolates, except in the epidermal growth factor-like domain at the carboxyl terminus where only one mutation was observed in one P. o. wallikeri isolate. Further, truncated PoMSP4 segments were successfully expressed and purified as ~ 32 kDa proteins. Importantly, high antibody responses with end-point titres ranging from 1:10,000 to 1:2,560,000 in all immunized mouse groups were observed, with high IgG avidity to PocMSP4 (80.5%) and PowMSP4 (92.3%). Furthermore, rPocMSP4 and rPowMSP4 cross-reacted with anti-PowMSP4-specific or anti-PocMSP4-specific antibodies. Additionally, anti-PoMSP4 IgG antibodies showed broad immuno-specificity in reacting against rPoMSP1 and rPoAMA1. ... |
format |
Article in Journal/Newspaper |
author |
Juliette Uwase Ruilin Chu Kokouvi Kassegne Yao Lei Feihu Shen Haitian Fu Yifan Sun Yinghua Xuan Jun Cao Yang Cheng |
author_facet |
Juliette Uwase Ruilin Chu Kokouvi Kassegne Yao Lei Feihu Shen Haitian Fu Yifan Sun Yinghua Xuan Jun Cao Yang Cheng |
author_sort |
Juliette Uwase |
title |
Immunogenicity analysis of conserved fragments in Plasmodium ovale species merozoite surface protein 4 |
title_short |
Immunogenicity analysis of conserved fragments in Plasmodium ovale species merozoite surface protein 4 |
title_full |
Immunogenicity analysis of conserved fragments in Plasmodium ovale species merozoite surface protein 4 |
title_fullStr |
Immunogenicity analysis of conserved fragments in Plasmodium ovale species merozoite surface protein 4 |
title_full_unstemmed |
Immunogenicity analysis of conserved fragments in Plasmodium ovale species merozoite surface protein 4 |
title_sort |
immunogenicity analysis of conserved fragments in plasmodium ovale species merozoite surface protein 4 |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-03207-7 https://doaj.org/article/efdaba21564347b4903ea184c35a940e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-11 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s12936-020-03207-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03207-7 1475-2875 https://doaj.org/article/efdaba21564347b4903ea184c35a940e |
op_doi |
https://doi.org/10.1186/s12936-020-03207-7 |
container_title |
Malaria Journal |
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19 |
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1 |
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1766346224269524992 |