Klebsiella species associated with bovine mastitis in Newfoundland.
Klebsiella spp. is a common cause of bovine mastitis, but information regarding its molecular epidemiology is lacking from many parts of the world. On using mass spectrometry and partial sequencing of the rpoB gene, it was found that over a one year study, K. variicola and Enterobacter cloacae were...
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ftdoajarticles:oai:doaj.org/article:ef88c6d637484cb4b0423b9fa9223c3e 2023-05-15T17:21:18+02:00 Klebsiella species associated with bovine mastitis in Newfoundland. Milka P Podder Laura Rogers Peter K Daley Greg P Keefe Hugh G Whitney Kapil Tahlan 2014-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0106518 https://doaj.org/article/ef88c6d637484cb4b0423b9fa9223c3e EN eng Public Library of Science (PLoS) https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25180510/?tool=EBI https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0106518 https://doaj.org/article/ef88c6d637484cb4b0423b9fa9223c3e PLoS ONE, Vol 9, Iss 9, p e106518 (2014) Medicine R Science Q article 2014 ftdoajarticles https://doi.org/10.1371/journal.pone.0106518 2022-12-31T13:42:47Z Klebsiella spp. is a common cause of bovine mastitis, but information regarding its molecular epidemiology is lacking from many parts of the world. On using mass spectrometry and partial sequencing of the rpoB gene, it was found that over a one year study, K. variicola and Enterobacter cloacae were misidentified as K. pneumoniae in a small number of clinical mastitis (CM) cases from Newfoundland. Results suggest that the currently used standard biochemical/phenotypic tests lack the sensitivity required to accurately discriminate among the three mentioned Gram negative bacteria. In addition, a single strain of K. variicola was associated with CM from one farm in the study as demonstrated by Random Amplified Polymorphic DNA (RAPD) PCR. To the best of our knowledge, K. variicola, which is normally found in the environment, has not been isolated previously from milk obtained from cows with CM. Therefore, it is possible that K. variicola was not detected in milk samples in the past due to the inability of standard tests to discriminate it from other Klebsiella species. Article in Journal/Newspaper Newfoundland Directory of Open Access Journals: DOAJ Articles PLoS ONE 9 9 e106518 |
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Directory of Open Access Journals: DOAJ Articles |
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topic |
Medicine R Science Q |
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Medicine R Science Q Milka P Podder Laura Rogers Peter K Daley Greg P Keefe Hugh G Whitney Kapil Tahlan Klebsiella species associated with bovine mastitis in Newfoundland. |
topic_facet |
Medicine R Science Q |
description |
Klebsiella spp. is a common cause of bovine mastitis, but information regarding its molecular epidemiology is lacking from many parts of the world. On using mass spectrometry and partial sequencing of the rpoB gene, it was found that over a one year study, K. variicola and Enterobacter cloacae were misidentified as K. pneumoniae in a small number of clinical mastitis (CM) cases from Newfoundland. Results suggest that the currently used standard biochemical/phenotypic tests lack the sensitivity required to accurately discriminate among the three mentioned Gram negative bacteria. In addition, a single strain of K. variicola was associated with CM from one farm in the study as demonstrated by Random Amplified Polymorphic DNA (RAPD) PCR. To the best of our knowledge, K. variicola, which is normally found in the environment, has not been isolated previously from milk obtained from cows with CM. Therefore, it is possible that K. variicola was not detected in milk samples in the past due to the inability of standard tests to discriminate it from other Klebsiella species. |
format |
Article in Journal/Newspaper |
author |
Milka P Podder Laura Rogers Peter K Daley Greg P Keefe Hugh G Whitney Kapil Tahlan |
author_facet |
Milka P Podder Laura Rogers Peter K Daley Greg P Keefe Hugh G Whitney Kapil Tahlan |
author_sort |
Milka P Podder |
title |
Klebsiella species associated with bovine mastitis in Newfoundland. |
title_short |
Klebsiella species associated with bovine mastitis in Newfoundland. |
title_full |
Klebsiella species associated with bovine mastitis in Newfoundland. |
title_fullStr |
Klebsiella species associated with bovine mastitis in Newfoundland. |
title_full_unstemmed |
Klebsiella species associated with bovine mastitis in Newfoundland. |
title_sort |
klebsiella species associated with bovine mastitis in newfoundland. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pone.0106518 https://doaj.org/article/ef88c6d637484cb4b0423b9fa9223c3e |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_source |
PLoS ONE, Vol 9, Iss 9, p e106518 (2014) |
op_relation |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25180510/?tool=EBI https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0106518 https://doaj.org/article/ef88c6d637484cb4b0423b9fa9223c3e |
op_doi |
https://doi.org/10.1371/journal.pone.0106518 |
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PLoS ONE |
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9 |
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9 |
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e106518 |
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