A transcriptomic view of the proteome variability of newborn and adult Bothrops jararaca snake venoms.
BACKGROUND: Snake bite is a neglected public health problem in communities in rural areas of several countries. Bothrops jararaca causes many snake bites in Brazil and previous studies have demonstrated that the pharmacological activities displayed by its venom undergo a significant ontogenetic shif...
Published in: | PLoS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2012
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0001554 https://doaj.org/article/ef87209ac43c45b5a1dcbd10a8d3e03b |
id |
ftdoajarticles:oai:doaj.org/article:ef87209ac43c45b5a1dcbd10a8d3e03b |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:ef87209ac43c45b5a1dcbd10a8d3e03b 2023-05-15T15:16:26+02:00 A transcriptomic view of the proteome variability of newborn and adult Bothrops jararaca snake venoms. André Zelanis Débora Andrade-Silva Marisa M Rocha Maria F Furtado Solange M T Serrano Inácio L M Junqueira-de-Azevedo Paulo Lee Ho 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001554 https://doaj.org/article/ef87209ac43c45b5a1dcbd10a8d3e03b EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3302817?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001554 https://doaj.org/article/ef87209ac43c45b5a1dcbd10a8d3e03b PLoS Neglected Tropical Diseases, Vol 6, Iss 3, p e1554 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001554 2022-12-31T05:19:34Z BACKGROUND: Snake bite is a neglected public health problem in communities in rural areas of several countries. Bothrops jararaca causes many snake bites in Brazil and previous studies have demonstrated that the pharmacological activities displayed by its venom undergo a significant ontogenetic shift. Similarly, the venom proteome of B. jararaca exhibits a considerable variation upon neonate to adult transition, which is associated with changes in diet from ectothermic prey in early life to endothermic prey in adulthood. Moreover, it has been shown that the Brazilian commercial antibothropic antivenom, which is produced by immunization with adult venom, is less effective in neutralizing newborn venom effects. On the other hand, venom gland transcripts of newborn snakes are poorly known since all transcriptomic studies have been carried out using mRNA from adult specimens. METHODS/PRINCIPAL FINDINGS: Here we analyzed venom gland cDNA libraries of newborn and adult B. jararaca in order to evaluate whether the variability demonstrated for its venom proteome and pharmacological activities was correlated with differences in the structure of toxin transcripts. The analysis revealed that the variability in B. jararaca venom gland transcriptomes is quantitative, as illustrated by the very high content of metalloproteinases in the newborn venom glands. Moreover, the variability is also characterized by the structural diversity of SVMP precursors found in newborn and adult transcriptomes. In the adult transcriptome, however, the content of metalloproteinase precursors considerably diminishes and the number of transcripts of serine proteinases, C-type lectins and bradykinin-potentiating peptides increase. Moreover, the comparison of the content of ESTs encoding toxins in adult male and female venom glands showed some gender-related differences. CONCLUSIONS/SIGNIFICANCE: We demonstrate a substantial shift in toxin transcripts upon snake development and a marked decrease in the metalloproteinase P-III/P-I class ratio which ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 6 3 e1554 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 André Zelanis Débora Andrade-Silva Marisa M Rocha Maria F Furtado Solange M T Serrano Inácio L M Junqueira-de-Azevedo Paulo Lee Ho A transcriptomic view of the proteome variability of newborn and adult Bothrops jararaca snake venoms. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Snake bite is a neglected public health problem in communities in rural areas of several countries. Bothrops jararaca causes many snake bites in Brazil and previous studies have demonstrated that the pharmacological activities displayed by its venom undergo a significant ontogenetic shift. Similarly, the venom proteome of B. jararaca exhibits a considerable variation upon neonate to adult transition, which is associated with changes in diet from ectothermic prey in early life to endothermic prey in adulthood. Moreover, it has been shown that the Brazilian commercial antibothropic antivenom, which is produced by immunization with adult venom, is less effective in neutralizing newborn venom effects. On the other hand, venom gland transcripts of newborn snakes are poorly known since all transcriptomic studies have been carried out using mRNA from adult specimens. METHODS/PRINCIPAL FINDINGS: Here we analyzed venom gland cDNA libraries of newborn and adult B. jararaca in order to evaluate whether the variability demonstrated for its venom proteome and pharmacological activities was correlated with differences in the structure of toxin transcripts. The analysis revealed that the variability in B. jararaca venom gland transcriptomes is quantitative, as illustrated by the very high content of metalloproteinases in the newborn venom glands. Moreover, the variability is also characterized by the structural diversity of SVMP precursors found in newborn and adult transcriptomes. In the adult transcriptome, however, the content of metalloproteinase precursors considerably diminishes and the number of transcripts of serine proteinases, C-type lectins and bradykinin-potentiating peptides increase. Moreover, the comparison of the content of ESTs encoding toxins in adult male and female venom glands showed some gender-related differences. CONCLUSIONS/SIGNIFICANCE: We demonstrate a substantial shift in toxin transcripts upon snake development and a marked decrease in the metalloproteinase P-III/P-I class ratio which ... |
format |
Article in Journal/Newspaper |
author |
André Zelanis Débora Andrade-Silva Marisa M Rocha Maria F Furtado Solange M T Serrano Inácio L M Junqueira-de-Azevedo Paulo Lee Ho |
author_facet |
André Zelanis Débora Andrade-Silva Marisa M Rocha Maria F Furtado Solange M T Serrano Inácio L M Junqueira-de-Azevedo Paulo Lee Ho |
author_sort |
André Zelanis |
title |
A transcriptomic view of the proteome variability of newborn and adult Bothrops jararaca snake venoms. |
title_short |
A transcriptomic view of the proteome variability of newborn and adult Bothrops jararaca snake venoms. |
title_full |
A transcriptomic view of the proteome variability of newborn and adult Bothrops jararaca snake venoms. |
title_fullStr |
A transcriptomic view of the proteome variability of newborn and adult Bothrops jararaca snake venoms. |
title_full_unstemmed |
A transcriptomic view of the proteome variability of newborn and adult Bothrops jararaca snake venoms. |
title_sort |
transcriptomic view of the proteome variability of newborn and adult bothrops jararaca snake venoms. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001554 https://doaj.org/article/ef87209ac43c45b5a1dcbd10a8d3e03b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 3, p e1554 (2012) |
op_relation |
http://europepmc.org/articles/PMC3302817?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001554 https://doaj.org/article/ef87209ac43c45b5a1dcbd10a8d3e03b |
op_doi |
https://doi.org/10.1371/journal.pntd.0001554 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
6 |
container_issue |
3 |
container_start_page |
e1554 |
_version_ |
1766346721114193920 |