Towards the use of a smartphone imaging-based tool for point-of-care detection of asymptomatic low-density malaria parasitaemia
Abstract Background Globally, there are over 200 million cases of malaria annually and over 400,000 deaths. Early and accurate detection of low-density parasitaemia and asymptomatic individuals is key to achieving the World Health Organization (WHO) 2030 sustainable development goals of reducing mal...
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ftdoajarticles:oai:doaj.org/article:ef3bc0c1edcd4525a95ec3ae6c77d1eb 2023-05-15T15:13:17+02:00 Towards the use of a smartphone imaging-based tool for point-of-care detection of asymptomatic low-density malaria parasitaemia Ashlee J. Colbert Katrina Co Giselle Lima-Cooper Dong Hoon Lee Katherine N. Clayton Steven T. Wereley Chandy C. John Jacqueline C. Linnes Tamara L. Kinzer-Ursem 2021-09-01T00:00:00Z https://doi.org/10.1186/s12936-021-03894-w https://doaj.org/article/ef3bc0c1edcd4525a95ec3ae6c77d1eb EN eng BMC https://doi.org/10.1186/s12936-021-03894-w https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03894-w 1475-2875 https://doaj.org/article/ef3bc0c1edcd4525a95ec3ae6c77d1eb Malaria Journal, Vol 20, Iss 1, Pp 1-13 (2021) Malaria Particle-diffusometry Nucleic-acid based tests Smartphone-detection LAMP-assay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1186/s12936-021-03894-w 2022-12-31T04:42:35Z Abstract Background Globally, there are over 200 million cases of malaria annually and over 400,000 deaths. Early and accurate detection of low-density parasitaemia and asymptomatic individuals is key to achieving the World Health Organization (WHO) 2030 sustainable development goals of reducing malaria-related deaths by 90% and eradication in 35 countries. Current rapid diagnostic tests are neither sensitive nor specific enough to detect the low parasite concentrations in the blood of asymptomatic individuals. Methods Here, an imaging-based sensing technique, particle diffusometry (PD), is combined with loop mediated isothermal amplification (LAMP) on a smartphone-enabled device to detect low levels of parasitaemia often associated with asymptomatic malaria. After amplification, PD quantifies the Brownian motion of fluorescent nanoparticles in the solution during a 30 s video taken on the phone. The resulting diffusion coefficient is used to detect the presence of Plasmodium DNA amplicons. The coefficients of known negative samples are compared to positive samples using a one-way ANOVA post-hoc Dunnett’s test for confirmation of amplification. Results As few as 3 parasite/µL of blood was detectable in 45 min without DNA extraction. Plasmodium falciparum parasites were detected from asymptomatic individuals’ whole blood samples with 89% sensitivity and 100% specificity when compared to quantitative polymerase chain reaction (qPCR). Conclusions PD-LAMP is of value for the detection of low density parasitaemia especially in areas where trained personnel may be scarce. The demonstration of this smartphone biosensor paired with the sensitivity of LAMP provides a proof of concept to achieve widespread asymptomatic malaria testing at the point of care. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 20 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Malaria Particle-diffusometry Nucleic-acid based tests Smartphone-detection LAMP-assay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Particle-diffusometry Nucleic-acid based tests Smartphone-detection LAMP-assay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Ashlee J. Colbert Katrina Co Giselle Lima-Cooper Dong Hoon Lee Katherine N. Clayton Steven T. Wereley Chandy C. John Jacqueline C. Linnes Tamara L. Kinzer-Ursem Towards the use of a smartphone imaging-based tool for point-of-care detection of asymptomatic low-density malaria parasitaemia |
topic_facet |
Malaria Particle-diffusometry Nucleic-acid based tests Smartphone-detection LAMP-assay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Globally, there are over 200 million cases of malaria annually and over 400,000 deaths. Early and accurate detection of low-density parasitaemia and asymptomatic individuals is key to achieving the World Health Organization (WHO) 2030 sustainable development goals of reducing malaria-related deaths by 90% and eradication in 35 countries. Current rapid diagnostic tests are neither sensitive nor specific enough to detect the low parasite concentrations in the blood of asymptomatic individuals. Methods Here, an imaging-based sensing technique, particle diffusometry (PD), is combined with loop mediated isothermal amplification (LAMP) on a smartphone-enabled device to detect low levels of parasitaemia often associated with asymptomatic malaria. After amplification, PD quantifies the Brownian motion of fluorescent nanoparticles in the solution during a 30 s video taken on the phone. The resulting diffusion coefficient is used to detect the presence of Plasmodium DNA amplicons. The coefficients of known negative samples are compared to positive samples using a one-way ANOVA post-hoc Dunnett’s test for confirmation of amplification. Results As few as 3 parasite/µL of blood was detectable in 45 min without DNA extraction. Plasmodium falciparum parasites were detected from asymptomatic individuals’ whole blood samples with 89% sensitivity and 100% specificity when compared to quantitative polymerase chain reaction (qPCR). Conclusions PD-LAMP is of value for the detection of low density parasitaemia especially in areas where trained personnel may be scarce. The demonstration of this smartphone biosensor paired with the sensitivity of LAMP provides a proof of concept to achieve widespread asymptomatic malaria testing at the point of care. |
format |
Article in Journal/Newspaper |
author |
Ashlee J. Colbert Katrina Co Giselle Lima-Cooper Dong Hoon Lee Katherine N. Clayton Steven T. Wereley Chandy C. John Jacqueline C. Linnes Tamara L. Kinzer-Ursem |
author_facet |
Ashlee J. Colbert Katrina Co Giselle Lima-Cooper Dong Hoon Lee Katherine N. Clayton Steven T. Wereley Chandy C. John Jacqueline C. Linnes Tamara L. Kinzer-Ursem |
author_sort |
Ashlee J. Colbert |
title |
Towards the use of a smartphone imaging-based tool for point-of-care detection of asymptomatic low-density malaria parasitaemia |
title_short |
Towards the use of a smartphone imaging-based tool for point-of-care detection of asymptomatic low-density malaria parasitaemia |
title_full |
Towards the use of a smartphone imaging-based tool for point-of-care detection of asymptomatic low-density malaria parasitaemia |
title_fullStr |
Towards the use of a smartphone imaging-based tool for point-of-care detection of asymptomatic low-density malaria parasitaemia |
title_full_unstemmed |
Towards the use of a smartphone imaging-based tool for point-of-care detection of asymptomatic low-density malaria parasitaemia |
title_sort |
towards the use of a smartphone imaging-based tool for point-of-care detection of asymptomatic low-density malaria parasitaemia |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s12936-021-03894-w https://doaj.org/article/ef3bc0c1edcd4525a95ec3ae6c77d1eb |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 20, Iss 1, Pp 1-13 (2021) |
op_relation |
https://doi.org/10.1186/s12936-021-03894-w https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03894-w 1475-2875 https://doaj.org/article/ef3bc0c1edcd4525a95ec3ae6c77d1eb |
op_doi |
https://doi.org/10.1186/s12936-021-03894-w |
container_title |
Malaria Journal |
container_volume |
20 |
container_issue |
1 |
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1766343851072552960 |