Rhodoliths holobionts in a changing ocean: host-microbes interactions mediate coralline algae resilience under ocean acidification
Abstract Background Life in the ocean will increasingly have to contend with a complex matrix of concurrent shifts in environmental properties that impact their physiology and control their life histories. Rhodoliths are coralline red algae (Corallinales, Rhodophyta) that are photosynthesizers, calc...
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ftdoajarticles:oai:doaj.org/article:ef216aa64bd44e1e8b91fb46463ae9d5 2023-05-15T17:50:07+02:00 Rhodoliths holobionts in a changing ocean: host-microbes interactions mediate coralline algae resilience under ocean acidification Giselle S. Cavalcanti Priya Shukla Megan Morris Bárbara Ribeiro Mariah Foley Michael P. Doane Cristiane C. Thompson Matthew S. Edwards Elizabeth A. Dinsdale Fabiano L. Thompson 2018-09-01T00:00:00Z https://doi.org/10.1186/s12864-018-5064-4 https://doaj.org/article/ef216aa64bd44e1e8b91fb46463ae9d5 EN eng BMC http://link.springer.com/article/10.1186/s12864-018-5064-4 https://doaj.org/toc/1471-2164 doi:10.1186/s12864-018-5064-4 1471-2164 https://doaj.org/article/ef216aa64bd44e1e8b91fb46463ae9d5 BMC Genomics, Vol 19, Iss 1, Pp 1-13 (2018) Climate change Coralline crustose algae Host-microbe interactions Metagenomics Ocean acidification Rhodolith Biotechnology TP248.13-248.65 Genetics QH426-470 article 2018 ftdoajarticles https://doi.org/10.1186/s12864-018-5064-4 2022-12-31T01:16:39Z Abstract Background Life in the ocean will increasingly have to contend with a complex matrix of concurrent shifts in environmental properties that impact their physiology and control their life histories. Rhodoliths are coralline red algae (Corallinales, Rhodophyta) that are photosynthesizers, calcifiers, and ecosystem engineers and therefore represent important targets for ocean acidification (OA) research. Here, we exposed live rhodoliths to near-future OA conditions to investigate responses in their photosynthetic capacity, calcium carbonate production, and associated microbiome using carbon uptake, decalcification assays, and whole genome shotgun sequencing metagenomic analysis, respectively. The results from our live rhodolith assays were compared to similar manipulations on dead rhodolith (calcareous skeleton) biofilms and water column microbial communities, thereby enabling the assessment of host-microbiome interaction under climate-driven environmental perturbations. Results Under high pCO2 conditions, live rhodoliths exhibited positive physiological responses, i.e. increased photosynthetic activity, and no calcium carbonate biomass loss over time. Further, whereas the microbiome associated with live rhodoliths remained stable and resembled a healthy holobiont, the microbial community associated with the water column changed after exposure to elevated pCO2. Conclusions Our results suggest that a tightly regulated microbial-host interaction, as evidenced by the stability of the rhodolith microbiome recorded here under OA-like conditions, is important for host resilience to environmental stress. This study extends the scarce comprehension of microbes associated with rhodolith beds and their reaction to increased pCO2, providing a more comprehensive approach to OA studies by assessing the host holobiont. Article in Journal/Newspaper Ocean acidification Directory of Open Access Journals: DOAJ Articles BMC Genomics 19 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Climate change Coralline crustose algae Host-microbe interactions Metagenomics Ocean acidification Rhodolith Biotechnology TP248.13-248.65 Genetics QH426-470 |
spellingShingle |
Climate change Coralline crustose algae Host-microbe interactions Metagenomics Ocean acidification Rhodolith Biotechnology TP248.13-248.65 Genetics QH426-470 Giselle S. Cavalcanti Priya Shukla Megan Morris Bárbara Ribeiro Mariah Foley Michael P. Doane Cristiane C. Thompson Matthew S. Edwards Elizabeth A. Dinsdale Fabiano L. Thompson Rhodoliths holobionts in a changing ocean: host-microbes interactions mediate coralline algae resilience under ocean acidification |
topic_facet |
Climate change Coralline crustose algae Host-microbe interactions Metagenomics Ocean acidification Rhodolith Biotechnology TP248.13-248.65 Genetics QH426-470 |
description |
Abstract Background Life in the ocean will increasingly have to contend with a complex matrix of concurrent shifts in environmental properties that impact their physiology and control their life histories. Rhodoliths are coralline red algae (Corallinales, Rhodophyta) that are photosynthesizers, calcifiers, and ecosystem engineers and therefore represent important targets for ocean acidification (OA) research. Here, we exposed live rhodoliths to near-future OA conditions to investigate responses in their photosynthetic capacity, calcium carbonate production, and associated microbiome using carbon uptake, decalcification assays, and whole genome shotgun sequencing metagenomic analysis, respectively. The results from our live rhodolith assays were compared to similar manipulations on dead rhodolith (calcareous skeleton) biofilms and water column microbial communities, thereby enabling the assessment of host-microbiome interaction under climate-driven environmental perturbations. Results Under high pCO2 conditions, live rhodoliths exhibited positive physiological responses, i.e. increased photosynthetic activity, and no calcium carbonate biomass loss over time. Further, whereas the microbiome associated with live rhodoliths remained stable and resembled a healthy holobiont, the microbial community associated with the water column changed after exposure to elevated pCO2. Conclusions Our results suggest that a tightly regulated microbial-host interaction, as evidenced by the stability of the rhodolith microbiome recorded here under OA-like conditions, is important for host resilience to environmental stress. This study extends the scarce comprehension of microbes associated with rhodolith beds and their reaction to increased pCO2, providing a more comprehensive approach to OA studies by assessing the host holobiont. |
format |
Article in Journal/Newspaper |
author |
Giselle S. Cavalcanti Priya Shukla Megan Morris Bárbara Ribeiro Mariah Foley Michael P. Doane Cristiane C. Thompson Matthew S. Edwards Elizabeth A. Dinsdale Fabiano L. Thompson |
author_facet |
Giselle S. Cavalcanti Priya Shukla Megan Morris Bárbara Ribeiro Mariah Foley Michael P. Doane Cristiane C. Thompson Matthew S. Edwards Elizabeth A. Dinsdale Fabiano L. Thompson |
author_sort |
Giselle S. Cavalcanti |
title |
Rhodoliths holobionts in a changing ocean: host-microbes interactions mediate coralline algae resilience under ocean acidification |
title_short |
Rhodoliths holobionts in a changing ocean: host-microbes interactions mediate coralline algae resilience under ocean acidification |
title_full |
Rhodoliths holobionts in a changing ocean: host-microbes interactions mediate coralline algae resilience under ocean acidification |
title_fullStr |
Rhodoliths holobionts in a changing ocean: host-microbes interactions mediate coralline algae resilience under ocean acidification |
title_full_unstemmed |
Rhodoliths holobionts in a changing ocean: host-microbes interactions mediate coralline algae resilience under ocean acidification |
title_sort |
rhodoliths holobionts in a changing ocean: host-microbes interactions mediate coralline algae resilience under ocean acidification |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s12864-018-5064-4 https://doaj.org/article/ef216aa64bd44e1e8b91fb46463ae9d5 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
BMC Genomics, Vol 19, Iss 1, Pp 1-13 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s12864-018-5064-4 https://doaj.org/toc/1471-2164 doi:10.1186/s12864-018-5064-4 1471-2164 https://doaj.org/article/ef216aa64bd44e1e8b91fb46463ae9d5 |
op_doi |
https://doi.org/10.1186/s12864-018-5064-4 |
container_title |
BMC Genomics |
container_volume |
19 |
container_issue |
1 |
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1766156717946568704 |