Comprehensive Characterization of Secondary Metabolites in Fruits and Leaves of Cloudberry ( Rubus chamaemorus L.)
Cloudberry ( Rubus chamaemorus L.) is a circumpolar boreal plant rich in bioactive compounds and is widely used in food and in folk medicine. In this study, a combination of two-dimensional NMR spectroscopy and liquid chromatography–high-resolution mass spectrometry was used for the comprehensive ch...
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ftdoajarticles:oai:doaj.org/article:ef10f3e7e1f24782a2787389d69b2892 2023-06-11T04:16:19+02:00 Comprehensive Characterization of Secondary Metabolites in Fruits and Leaves of Cloudberry ( Rubus chamaemorus L.) Anna V. Faleva Nikolay V. Ul’yanovskii Aleksandra A. Onuchina Danil I. Falev Dmitry S. Kosyakov 2023-04-01T00:00:00Z https://doi.org/10.3390/metabo13050598 https://doaj.org/article/ef10f3e7e1f24782a2787389d69b2892 EN eng MDPI AG https://www.mdpi.com/2218-1989/13/5/598 https://doaj.org/toc/2218-1989 doi:10.3390/metabo13050598 2218-1989 https://doaj.org/article/ef10f3e7e1f24782a2787389d69b2892 Metabolites, Vol 13, Iss 598, p 598 (2023) Rubus chamaemorus cloudberry secondary metabolites extractives polyphenols antioxidant activity Microbiology QR1-502 article 2023 ftdoajarticles https://doi.org/10.3390/metabo13050598 2023-05-28T00:33:35Z Cloudberry ( Rubus chamaemorus L.) is a circumpolar boreal plant rich in bioactive compounds and is widely used in food and in folk medicine. In this study, a combination of two-dimensional NMR spectroscopy and liquid chromatography–high-resolution mass spectrometry was used for the comprehensive characterization of secondary metabolites in cloudberry lipophilic and hydrophilic extracts. Special attention was paid to the leaf extractives, which are highly enriched in polyphenolic compounds, the content of which reaches 19% in the extract (in gallic acid equivalent). The chemical composition of the polyphenolic fraction is represented mainly by the glycosylated derivatives of flavonoids, hydroxycinnamic (primarily caffeic), gallic (including the structure of galloyl ascorbate) and ellagic acids, catechin, and procyanidins. The contents of aglycones in the polyphenolic fraction were 64 and 100 mg g −1 for flavonoids and hydroxycinnamic acids, respectively, while the content of free caffeic acid was 1.2 mg g −1 . This determines the exceptionally high antioxidant activity of this fraction (750 mg g −1 in gallic acid equivalent) and the ability to scavenge superoxide anion radicals, which is 60% higher than that of Trolox. The lower polar fractions consist mainly of glycolipids, which include polyunsaturated linolenic acid (18:3), pentacyclic triterpenic acids, carotenoid lutein, and chlorophyll derivatives, among which pheophytin a dominates. Along with the availability, the high antioxidant and biological activities of cloudberry leaf extracts make them a promising source of food additives, cosmetics, and pharmaceuticals. Article in Journal/Newspaper Rubus chamaemorus Directory of Open Access Journals: DOAJ Articles Metabolites 13 5 598 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Rubus chamaemorus cloudberry secondary metabolites extractives polyphenols antioxidant activity Microbiology QR1-502 |
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Rubus chamaemorus cloudberry secondary metabolites extractives polyphenols antioxidant activity Microbiology QR1-502 Anna V. Faleva Nikolay V. Ul’yanovskii Aleksandra A. Onuchina Danil I. Falev Dmitry S. Kosyakov Comprehensive Characterization of Secondary Metabolites in Fruits and Leaves of Cloudberry ( Rubus chamaemorus L.) |
topic_facet |
Rubus chamaemorus cloudberry secondary metabolites extractives polyphenols antioxidant activity Microbiology QR1-502 |
description |
Cloudberry ( Rubus chamaemorus L.) is a circumpolar boreal plant rich in bioactive compounds and is widely used in food and in folk medicine. In this study, a combination of two-dimensional NMR spectroscopy and liquid chromatography–high-resolution mass spectrometry was used for the comprehensive characterization of secondary metabolites in cloudberry lipophilic and hydrophilic extracts. Special attention was paid to the leaf extractives, which are highly enriched in polyphenolic compounds, the content of which reaches 19% in the extract (in gallic acid equivalent). The chemical composition of the polyphenolic fraction is represented mainly by the glycosylated derivatives of flavonoids, hydroxycinnamic (primarily caffeic), gallic (including the structure of galloyl ascorbate) and ellagic acids, catechin, and procyanidins. The contents of aglycones in the polyphenolic fraction were 64 and 100 mg g −1 for flavonoids and hydroxycinnamic acids, respectively, while the content of free caffeic acid was 1.2 mg g −1 . This determines the exceptionally high antioxidant activity of this fraction (750 mg g −1 in gallic acid equivalent) and the ability to scavenge superoxide anion radicals, which is 60% higher than that of Trolox. The lower polar fractions consist mainly of glycolipids, which include polyunsaturated linolenic acid (18:3), pentacyclic triterpenic acids, carotenoid lutein, and chlorophyll derivatives, among which pheophytin a dominates. Along with the availability, the high antioxidant and biological activities of cloudberry leaf extracts make them a promising source of food additives, cosmetics, and pharmaceuticals. |
format |
Article in Journal/Newspaper |
author |
Anna V. Faleva Nikolay V. Ul’yanovskii Aleksandra A. Onuchina Danil I. Falev Dmitry S. Kosyakov |
author_facet |
Anna V. Faleva Nikolay V. Ul’yanovskii Aleksandra A. Onuchina Danil I. Falev Dmitry S. Kosyakov |
author_sort |
Anna V. Faleva |
title |
Comprehensive Characterization of Secondary Metabolites in Fruits and Leaves of Cloudberry ( Rubus chamaemorus L.) |
title_short |
Comprehensive Characterization of Secondary Metabolites in Fruits and Leaves of Cloudberry ( Rubus chamaemorus L.) |
title_full |
Comprehensive Characterization of Secondary Metabolites in Fruits and Leaves of Cloudberry ( Rubus chamaemorus L.) |
title_fullStr |
Comprehensive Characterization of Secondary Metabolites in Fruits and Leaves of Cloudberry ( Rubus chamaemorus L.) |
title_full_unstemmed |
Comprehensive Characterization of Secondary Metabolites in Fruits and Leaves of Cloudberry ( Rubus chamaemorus L.) |
title_sort |
comprehensive characterization of secondary metabolites in fruits and leaves of cloudberry ( rubus chamaemorus l.) |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/metabo13050598 https://doaj.org/article/ef10f3e7e1f24782a2787389d69b2892 |
genre |
Rubus chamaemorus |
genre_facet |
Rubus chamaemorus |
op_source |
Metabolites, Vol 13, Iss 598, p 598 (2023) |
op_relation |
https://www.mdpi.com/2218-1989/13/5/598 https://doaj.org/toc/2218-1989 doi:10.3390/metabo13050598 2218-1989 https://doaj.org/article/ef10f3e7e1f24782a2787389d69b2892 |
op_doi |
https://doi.org/10.3390/metabo13050598 |
container_title |
Metabolites |
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13 |
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5 |
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598 |
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1768373963241553920 |