Leishmania infantum INFECTION IN DOGS FROM THE SOUTHERN REGION OF MINAS GERAIS STATE, BRAZIL
SUMMARY Visceral leishmaniasis is a systemic and chronic disease and dogs are the main reservoir of the etiologic agent, Leishmania infantum (syn L. chagasi). A serological and molecular investigation of canine visceral leishmaniasis (CVL) was performed in the municipality of Alfenas, located in the...
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ftdoajarticles:oai:doaj.org/article:eef54aa0dd96458ab54b484104c4967c 2024-09-09T19:27:16+00:00 Leishmania infantum INFECTION IN DOGS FROM THE SOUTHERN REGION OF MINAS GERAIS STATE, BRAZIL Juliana Barbosa NUNES Márcia Dalastra LAURENTI Herminia Yohko KANAMURA Alessandro Antônio Costa PEREIRA Fabio Antonio COLOMBO Marcos José MARQUES https://doi.org/10.1590/S1678-9946201658075 https://doaj.org/article/eef54aa0dd96458ab54b484104c4967c EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652016005000257&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S1678-9946201658075 https://doaj.org/article/eef54aa0dd96458ab54b484104c4967c Revista do Instituto de Medicina Tropical de São Paulo, Vol 58, Iss 0 Canine visceral leishmaniasis Leishmania infantum Serology Polymerase chain reaction Sequencing Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article ftdoajarticles https://doi.org/10.1590/S1678-9946201658075 2024-08-05T17:49:30Z SUMMARY Visceral leishmaniasis is a systemic and chronic disease and dogs are the main reservoir of the etiologic agent, Leishmania infantum (syn L. chagasi). A serological and molecular investigation of canine visceral leishmaniasis (CVL) was performed in the municipality of Alfenas, located in the southern region of Minas Gerais, where the disease is not endemic. Samples from 87 dogs were submitted to serological tests including the Dual Path Platform (DPP (r) ) CVL Bio-Manguinhos rapid test, an in-house enzyme-linked immunosorbent assay (ELISA) and an immunofluorescence antibody test (IFAT), as well as molecular techniques such as a conventional polymerase chain reaction (PCR) with the RV1/RV2 primers and a quantitative PCR (qPCR) with the LinJ31, Ldon and DNApol primers. Of the 87 serum samples, eight (9.2%) were positive for Leishmania using the DPP rapid test, but only four (4.6%) were confirmed by ELISA and two (2.3%) by IFAT. In these two serologically confirmed cases, spleen and liver samples were positive by all the employed molecular and parasitological procedures performed on spleen samples. When whole blood samples were used in the molecular assays, two samples (2.3%) were positive only by qPCR. DNA extracted and amplified from the spleens of seropositive dogs was sequenced, showing 100% of similarity with the Leishmania infantum (syn L. chagasi) sequence. Thus, the first cases of CVL have been confirmed in the Alfenas region, suggesting the importance of canine surveys in non-endemic municipalities for CVL to monitor disease progression and to prevent outbreaks. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 58 0 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Canine visceral leishmaniasis Leishmania infantum Serology Polymerase chain reaction Sequencing Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Canine visceral leishmaniasis Leishmania infantum Serology Polymerase chain reaction Sequencing Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Juliana Barbosa NUNES Márcia Dalastra LAURENTI Herminia Yohko KANAMURA Alessandro Antônio Costa PEREIRA Fabio Antonio COLOMBO Marcos José MARQUES Leishmania infantum INFECTION IN DOGS FROM THE SOUTHERN REGION OF MINAS GERAIS STATE, BRAZIL |
topic_facet |
Canine visceral leishmaniasis Leishmania infantum Serology Polymerase chain reaction Sequencing Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
SUMMARY Visceral leishmaniasis is a systemic and chronic disease and dogs are the main reservoir of the etiologic agent, Leishmania infantum (syn L. chagasi). A serological and molecular investigation of canine visceral leishmaniasis (CVL) was performed in the municipality of Alfenas, located in the southern region of Minas Gerais, where the disease is not endemic. Samples from 87 dogs were submitted to serological tests including the Dual Path Platform (DPP (r) ) CVL Bio-Manguinhos rapid test, an in-house enzyme-linked immunosorbent assay (ELISA) and an immunofluorescence antibody test (IFAT), as well as molecular techniques such as a conventional polymerase chain reaction (PCR) with the RV1/RV2 primers and a quantitative PCR (qPCR) with the LinJ31, Ldon and DNApol primers. Of the 87 serum samples, eight (9.2%) were positive for Leishmania using the DPP rapid test, but only four (4.6%) were confirmed by ELISA and two (2.3%) by IFAT. In these two serologically confirmed cases, spleen and liver samples were positive by all the employed molecular and parasitological procedures performed on spleen samples. When whole blood samples were used in the molecular assays, two samples (2.3%) were positive only by qPCR. DNA extracted and amplified from the spleens of seropositive dogs was sequenced, showing 100% of similarity with the Leishmania infantum (syn L. chagasi) sequence. Thus, the first cases of CVL have been confirmed in the Alfenas region, suggesting the importance of canine surveys in non-endemic municipalities for CVL to monitor disease progression and to prevent outbreaks. |
format |
Article in Journal/Newspaper |
author |
Juliana Barbosa NUNES Márcia Dalastra LAURENTI Herminia Yohko KANAMURA Alessandro Antônio Costa PEREIRA Fabio Antonio COLOMBO Marcos José MARQUES |
author_facet |
Juliana Barbosa NUNES Márcia Dalastra LAURENTI Herminia Yohko KANAMURA Alessandro Antônio Costa PEREIRA Fabio Antonio COLOMBO Marcos José MARQUES |
author_sort |
Juliana Barbosa NUNES |
title |
Leishmania infantum INFECTION IN DOGS FROM THE SOUTHERN REGION OF MINAS GERAIS STATE, BRAZIL |
title_short |
Leishmania infantum INFECTION IN DOGS FROM THE SOUTHERN REGION OF MINAS GERAIS STATE, BRAZIL |
title_full |
Leishmania infantum INFECTION IN DOGS FROM THE SOUTHERN REGION OF MINAS GERAIS STATE, BRAZIL |
title_fullStr |
Leishmania infantum INFECTION IN DOGS FROM THE SOUTHERN REGION OF MINAS GERAIS STATE, BRAZIL |
title_full_unstemmed |
Leishmania infantum INFECTION IN DOGS FROM THE SOUTHERN REGION OF MINAS GERAIS STATE, BRAZIL |
title_sort |
leishmania infantum infection in dogs from the southern region of minas gerais state, brazil |
publisher |
Universidade de São Paulo (USP) |
url |
https://doi.org/10.1590/S1678-9946201658075 https://doaj.org/article/eef54aa0dd96458ab54b484104c4967c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 58, Iss 0 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652016005000257&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S1678-9946201658075 https://doaj.org/article/eef54aa0dd96458ab54b484104c4967c |
op_doi |
https://doi.org/10.1590/S1678-9946201658075 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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58 |
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1809896723765526528 |