Detection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test targeting the groEL gene
Abstract Background Candidatus Neoehrlichia mikurensis is an emerging tick-borne pathogen. It is widely distributed in Ixodes ricinus ticks in Europe, but knowledge of its distribution in Norway, where I. ricinus reaches its northern limit, is limited. In this study we have developed a real time PCR...
Published in: | BMC Microbiology |
---|---|
Main Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2019
|
Subjects: | |
Online Access: | https://doi.org/10.1186/s12866-019-1502-y https://doaj.org/article/eebcff7025a848499b9e2acaf50dd784 |
id |
ftdoajarticles:oai:doaj.org/article:eebcff7025a848499b9e2acaf50dd784 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:eebcff7025a848499b9e2acaf50dd784 2023-05-15T14:59:53+02:00 Detection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test targeting the groEL gene Andrew Jenkins Cecilie Raasok Benedikte N. Pedersen Kristine Jensen Åshild Andreassen Arnulf Soleng Kristin Skarsfjord Edgar Heidi Heggen Lindstedt Vivian Kjelland Snorre Stuen Dag Hvidsten Bjørn-Erik Kristiansen 2019-08-01T00:00:00Z https://doi.org/10.1186/s12866-019-1502-y https://doaj.org/article/eebcff7025a848499b9e2acaf50dd784 EN eng BMC http://link.springer.com/article/10.1186/s12866-019-1502-y https://doaj.org/toc/1471-2180 doi:10.1186/s12866-019-1502-y 1471-2180 https://doaj.org/article/eebcff7025a848499b9e2acaf50dd784 BMC Microbiology, Vol 19, Iss 1, Pp 1-12 (2019) Neoehrlichia mikurensis Ixodes ricinus Ticks Tick-borne diseases Norway Scandinavia Microbiology QR1-502 article 2019 ftdoajarticles https://doi.org/10.1186/s12866-019-1502-y 2022-12-31T09:54:48Z Abstract Background Candidatus Neoehrlichia mikurensis is an emerging tick-borne pathogen. It is widely distributed in Ixodes ricinus ticks in Europe, but knowledge of its distribution in Norway, where I. ricinus reaches its northern limit, is limited. In this study we have developed a real time PCR test for Ca. N. mikurensis and used it to investigate the distribution of Ca. N. mikurensis in Norway. Results Real time PCR targeting the groEL gene was developed and shown to be highly sensitive. It was used to detect Ca. N. mikurensis in 1651 I. ricinus nymphs and adults collected from twelve locations in Norway, from the eastern Oslo Fjord in the south to near the Arctic Circle in the north. The overall prevalence was 6.5% and varied locally between 0 and 16%. Prevalence in adults and nymphs was similar, suggesting that ticks acquire Ca. N. mikurensis predominantly during their first blood meal. In addition, 123 larvae were investigated; Ca. N. mikurensis was not found in larvae, suggesting that transovarial transmission is rare or absent. Sequence analysis suggests that a single variant dominates in Norway. Conclusions Ca. N. mikurensis is widespread and common in ticks in Norway and reaches up to their northern limit near the Arctic Circle. Ticks appear to acquire Ca. N. mikurensis during their first blood meal. No evidence for transovarial transmission was found. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Norway BMC Microbiology 19 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Neoehrlichia mikurensis Ixodes ricinus Ticks Tick-borne diseases Norway Scandinavia Microbiology QR1-502 |
spellingShingle |
Neoehrlichia mikurensis Ixodes ricinus Ticks Tick-borne diseases Norway Scandinavia Microbiology QR1-502 Andrew Jenkins Cecilie Raasok Benedikte N. Pedersen Kristine Jensen Åshild Andreassen Arnulf Soleng Kristin Skarsfjord Edgar Heidi Heggen Lindstedt Vivian Kjelland Snorre Stuen Dag Hvidsten Bjørn-Erik Kristiansen Detection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test targeting the groEL gene |
topic_facet |
Neoehrlichia mikurensis Ixodes ricinus Ticks Tick-borne diseases Norway Scandinavia Microbiology QR1-502 |
description |
Abstract Background Candidatus Neoehrlichia mikurensis is an emerging tick-borne pathogen. It is widely distributed in Ixodes ricinus ticks in Europe, but knowledge of its distribution in Norway, where I. ricinus reaches its northern limit, is limited. In this study we have developed a real time PCR test for Ca. N. mikurensis and used it to investigate the distribution of Ca. N. mikurensis in Norway. Results Real time PCR targeting the groEL gene was developed and shown to be highly sensitive. It was used to detect Ca. N. mikurensis in 1651 I. ricinus nymphs and adults collected from twelve locations in Norway, from the eastern Oslo Fjord in the south to near the Arctic Circle in the north. The overall prevalence was 6.5% and varied locally between 0 and 16%. Prevalence in adults and nymphs was similar, suggesting that ticks acquire Ca. N. mikurensis predominantly during their first blood meal. In addition, 123 larvae were investigated; Ca. N. mikurensis was not found in larvae, suggesting that transovarial transmission is rare or absent. Sequence analysis suggests that a single variant dominates in Norway. Conclusions Ca. N. mikurensis is widespread and common in ticks in Norway and reaches up to their northern limit near the Arctic Circle. Ticks appear to acquire Ca. N. mikurensis during their first blood meal. No evidence for transovarial transmission was found. |
format |
Article in Journal/Newspaper |
author |
Andrew Jenkins Cecilie Raasok Benedikte N. Pedersen Kristine Jensen Åshild Andreassen Arnulf Soleng Kristin Skarsfjord Edgar Heidi Heggen Lindstedt Vivian Kjelland Snorre Stuen Dag Hvidsten Bjørn-Erik Kristiansen |
author_facet |
Andrew Jenkins Cecilie Raasok Benedikte N. Pedersen Kristine Jensen Åshild Andreassen Arnulf Soleng Kristin Skarsfjord Edgar Heidi Heggen Lindstedt Vivian Kjelland Snorre Stuen Dag Hvidsten Bjørn-Erik Kristiansen |
author_sort |
Andrew Jenkins |
title |
Detection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test targeting the groEL gene |
title_short |
Detection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test targeting the groEL gene |
title_full |
Detection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test targeting the groEL gene |
title_fullStr |
Detection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test targeting the groEL gene |
title_full_unstemmed |
Detection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test targeting the groEL gene |
title_sort |
detection of candidatus neoehrlichia mikurensis in norway up to the northern limit of ixodes ricinus distribution using a novel real time pcr test targeting the groel gene |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12866-019-1502-y https://doaj.org/article/eebcff7025a848499b9e2acaf50dd784 |
geographic |
Arctic Norway |
geographic_facet |
Arctic Norway |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
BMC Microbiology, Vol 19, Iss 1, Pp 1-12 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s12866-019-1502-y https://doaj.org/toc/1471-2180 doi:10.1186/s12866-019-1502-y 1471-2180 https://doaj.org/article/eebcff7025a848499b9e2acaf50dd784 |
op_doi |
https://doi.org/10.1186/s12866-019-1502-y |
container_title |
BMC Microbiology |
container_volume |
19 |
container_issue |
1 |
_version_ |
1766332002978496512 |