Species Identification of Birds Nasal Trichobilharzia in Sari, North of Iran
Background: Cercarial dermatitis is known as an endemic parasitic disease in North of Iran, a hypersensitive skin reaction to the penetration of nonhuman schistosome larvae into human skin. In recent studies in this region, final and intermediate hosts were recognized and Trichobilharzia was identi...
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Tehran University of Medical Sciences
2012
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ftdoajarticles:oai:doaj.org/article:eea58d8fa2cc48d194a0fe3bd093178d 2023-05-15T13:25:00+02:00 Species Identification of Birds Nasal Trichobilharzia in Sari, North of Iran SH Gohardehi N Taghipour A Haghighi A Athari SH Maleki S Seyyed Tabaei 2012-12-01T00:00:00Z https://doaj.org/article/eea58d8fa2cc48d194a0fe3bd093178d EN eng Tehran University of Medical Sciences http://ijpa.tums.ac.ir/index.php/ijpa/article/view/473/347 https://doaj.org/toc/1735-7020 https://doaj.org/toc/2008-238X 1735-7020 2008-238X https://doaj.org/article/eea58d8fa2cc48d194a0fe3bd093178d Iranian Journal of Parasitology, Vol 7, Iss 4, Pp 82-85 (2012) Trichobilharzia Regenti Cercarial Dermatitis PCR Iran Anatid Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles 2022-12-31T05:45:22Z Background: Cercarial dermatitis is known as an endemic parasitic disease in North of Iran, a hypersensitive skin reaction to the penetration of nonhuman schistosome larvae into human skin. In recent studies in this region, final and intermediate hosts were recognized and Trichobilharzia was identified as the main causative agent of cercarial dermatitis in this region, but to date the parasite species haven’t been identified. Therefore this study was performed to species identification of nasal Trichobilharzia in infected birds for the first time.Methods: A total of 45 Anas clypeata birds identified as final host, were collected from Sari in North of Iran and infected nasal tissues analyzed using molecular techniques. Genomic DNA was isolated by phenol/chloroform extraction method and ITS region of rDNA were amplified with specific primers its5Trem and its4Trem, then sequenced area were compared with existing records in GenBank.Results: Twelve samples were infected with Trichobilharzia and results of PCR reaction indicated that all of them belonged to T. regenti. The sequence alignment of present work isolates and those deposited in GenBank showed differences in nucleotide sequences of repeat region in ITS1.Conclusion: Trichobilharzia regenti is the most frequent parasite of Anatid birds in North of Iran. This corresponds to the distribution of this parasite along the flyway of migratory birds, which annually migrate from Siberia and northern countries of Caspian Sea to wintering areas in southern regions of it. Article in Journal/Newspaper Anas clypeata Siberia Directory of Open Access Journals: DOAJ Articles |
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English |
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Trichobilharzia Regenti Cercarial Dermatitis PCR Iran Anatid Infectious and parasitic diseases RC109-216 |
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Trichobilharzia Regenti Cercarial Dermatitis PCR Iran Anatid Infectious and parasitic diseases RC109-216 SH Gohardehi N Taghipour A Haghighi A Athari SH Maleki S Seyyed Tabaei Species Identification of Birds Nasal Trichobilharzia in Sari, North of Iran |
topic_facet |
Trichobilharzia Regenti Cercarial Dermatitis PCR Iran Anatid Infectious and parasitic diseases RC109-216 |
description |
Background: Cercarial dermatitis is known as an endemic parasitic disease in North of Iran, a hypersensitive skin reaction to the penetration of nonhuman schistosome larvae into human skin. In recent studies in this region, final and intermediate hosts were recognized and Trichobilharzia was identified as the main causative agent of cercarial dermatitis in this region, but to date the parasite species haven’t been identified. Therefore this study was performed to species identification of nasal Trichobilharzia in infected birds for the first time.Methods: A total of 45 Anas clypeata birds identified as final host, were collected from Sari in North of Iran and infected nasal tissues analyzed using molecular techniques. Genomic DNA was isolated by phenol/chloroform extraction method and ITS region of rDNA were amplified with specific primers its5Trem and its4Trem, then sequenced area were compared with existing records in GenBank.Results: Twelve samples were infected with Trichobilharzia and results of PCR reaction indicated that all of them belonged to T. regenti. The sequence alignment of present work isolates and those deposited in GenBank showed differences in nucleotide sequences of repeat region in ITS1.Conclusion: Trichobilharzia regenti is the most frequent parasite of Anatid birds in North of Iran. This corresponds to the distribution of this parasite along the flyway of migratory birds, which annually migrate from Siberia and northern countries of Caspian Sea to wintering areas in southern regions of it. |
format |
Article in Journal/Newspaper |
author |
SH Gohardehi N Taghipour A Haghighi A Athari SH Maleki S Seyyed Tabaei |
author_facet |
SH Gohardehi N Taghipour A Haghighi A Athari SH Maleki S Seyyed Tabaei |
author_sort |
SH Gohardehi |
title |
Species Identification of Birds Nasal Trichobilharzia in Sari, North of Iran |
title_short |
Species Identification of Birds Nasal Trichobilharzia in Sari, North of Iran |
title_full |
Species Identification of Birds Nasal Trichobilharzia in Sari, North of Iran |
title_fullStr |
Species Identification of Birds Nasal Trichobilharzia in Sari, North of Iran |
title_full_unstemmed |
Species Identification of Birds Nasal Trichobilharzia in Sari, North of Iran |
title_sort |
species identification of birds nasal trichobilharzia in sari, north of iran |
publisher |
Tehran University of Medical Sciences |
publishDate |
2012 |
url |
https://doaj.org/article/eea58d8fa2cc48d194a0fe3bd093178d |
genre |
Anas clypeata Siberia |
genre_facet |
Anas clypeata Siberia |
op_source |
Iranian Journal of Parasitology, Vol 7, Iss 4, Pp 82-85 (2012) |
op_relation |
http://ijpa.tums.ac.ir/index.php/ijpa/article/view/473/347 https://doaj.org/toc/1735-7020 https://doaj.org/toc/2008-238X 1735-7020 2008-238X https://doaj.org/article/eea58d8fa2cc48d194a0fe3bd093178d |
_version_ |
1766383021888372736 |