Antiproliferative activity of marine stingray Dasyatis sephenvenom on human cervical carcinoma cell line
AbstractBackgroundVenoms comprise mixtures of numerous bioactive compounds that have a wide range of pharmacologic actions. Toxins from venomous animals have attracted the attention of researchers because of their affinity for primary sites responsible for lethality and their efficacy at extremely l...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:ee9169a1a38d4193bfe24e71e1440154 2023-05-15T15:10:40+02:00 Antiproliferative activity of marine stingray Dasyatis sephenvenom on human cervical carcinoma cell line RK Rajeshkumar R Vennila S Karthikeyan N Rajendra Prasad M Arumugam T Velpandian T Balasubramaniam 2015-11-01T00:00:00Z https://doi.org/10.1186/s40409-015-0036-5 https://doaj.org/article/ee9169a1a38d4193bfe24e71e1440154 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100345&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/s40409-015-0036-5 https://doaj.org/article/ee9169a1a38d4193bfe24e71e1440154 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 21, Iss 0, Pp 1-1 (2015) Marine organisms Membrane potential Oxidative stress ROS Stingray Venom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2015 ftdoajarticles https://doi.org/10.1186/s40409-015-0036-5 2022-12-31T14:12:39Z AbstractBackgroundVenoms comprise mixtures of numerous bioactive compounds that have a wide range of pharmacologic actions. Toxins from venomous animals have attracted the attention of researchers because of their affinity for primary sites responsible for lethality and their efficacy at extremely low concentrations. The venoms of marine stingrays have not been extensively studied and limited data is available on them. The present study aims to evaluate the antiproliferative and biochemical properties of the venom obtained from a species of marine stingray (Dasyatis sephen) on human cervical cancer cell line HeLa.MethodsThe antiproliferative effect of D. sephen venom was determined by MTT assay, and the oxidative stress was determined by lipid peroxidation method along with assessment of changes in the enzymatic and non-enzymatic antioxidant status. We observed intracellular reactive oxygen species (ROS) levels by DCFH-DA method, mitochondrial membrane potential alterations by rhodamine 123 staining and apoptotic morphological changes by acridine orange/ethidium bromide dual staining method.ResultsD. sephen venom enhances lipid peroxidative markers such as thiobarbituric acid reactive substance, conjugated diene, and lipid hydroperoxide in HeLa cell lines. Stingray venom enhances the ROS levels, which is evidenced by the increased 2–7-diacetyl dichlorofluorescein fluorescence. Further, D. sephen venom treatment altered the mitochondrial membrane potential in HeLa cells. Additionally, we observed increased apoptotic morphological changes in D. sephen venom-treated groups. ConclusionsDasyatis sephen venom exhibits potent antiproliferative effect on HeLa cell line and upon further purification it could be a promising antiproliferative agent. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 21 1 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Marine organisms Membrane potential Oxidative stress ROS Stingray Venom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Marine organisms Membrane potential Oxidative stress ROS Stingray Venom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 RK Rajeshkumar R Vennila S Karthikeyan N Rajendra Prasad M Arumugam T Velpandian T Balasubramaniam Antiproliferative activity of marine stingray Dasyatis sephenvenom on human cervical carcinoma cell line |
topic_facet |
Marine organisms Membrane potential Oxidative stress ROS Stingray Venom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
AbstractBackgroundVenoms comprise mixtures of numerous bioactive compounds that have a wide range of pharmacologic actions. Toxins from venomous animals have attracted the attention of researchers because of their affinity for primary sites responsible for lethality and their efficacy at extremely low concentrations. The venoms of marine stingrays have not been extensively studied and limited data is available on them. The present study aims to evaluate the antiproliferative and biochemical properties of the venom obtained from a species of marine stingray (Dasyatis sephen) on human cervical cancer cell line HeLa.MethodsThe antiproliferative effect of D. sephen venom was determined by MTT assay, and the oxidative stress was determined by lipid peroxidation method along with assessment of changes in the enzymatic and non-enzymatic antioxidant status. We observed intracellular reactive oxygen species (ROS) levels by DCFH-DA method, mitochondrial membrane potential alterations by rhodamine 123 staining and apoptotic morphological changes by acridine orange/ethidium bromide dual staining method.ResultsD. sephen venom enhances lipid peroxidative markers such as thiobarbituric acid reactive substance, conjugated diene, and lipid hydroperoxide in HeLa cell lines. Stingray venom enhances the ROS levels, which is evidenced by the increased 2–7-diacetyl dichlorofluorescein fluorescence. Further, D. sephen venom treatment altered the mitochondrial membrane potential in HeLa cells. Additionally, we observed increased apoptotic morphological changes in D. sephen venom-treated groups. ConclusionsDasyatis sephen venom exhibits potent antiproliferative effect on HeLa cell line and upon further purification it could be a promising antiproliferative agent. |
format |
Article in Journal/Newspaper |
author |
RK Rajeshkumar R Vennila S Karthikeyan N Rajendra Prasad M Arumugam T Velpandian T Balasubramaniam |
author_facet |
RK Rajeshkumar R Vennila S Karthikeyan N Rajendra Prasad M Arumugam T Velpandian T Balasubramaniam |
author_sort |
RK Rajeshkumar |
title |
Antiproliferative activity of marine stingray Dasyatis sephenvenom on human cervical carcinoma cell line |
title_short |
Antiproliferative activity of marine stingray Dasyatis sephenvenom on human cervical carcinoma cell line |
title_full |
Antiproliferative activity of marine stingray Dasyatis sephenvenom on human cervical carcinoma cell line |
title_fullStr |
Antiproliferative activity of marine stingray Dasyatis sephenvenom on human cervical carcinoma cell line |
title_full_unstemmed |
Antiproliferative activity of marine stingray Dasyatis sephenvenom on human cervical carcinoma cell line |
title_sort |
antiproliferative activity of marine stingray dasyatis sephenvenom on human cervical carcinoma cell line |
publisher |
SciELO |
publishDate |
2015 |
url |
https://doi.org/10.1186/s40409-015-0036-5 https://doaj.org/article/ee9169a1a38d4193bfe24e71e1440154 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 21, Iss 0, Pp 1-1 (2015) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100345&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/s40409-015-0036-5 https://doaj.org/article/ee9169a1a38d4193bfe24e71e1440154 |
op_doi |
https://doi.org/10.1186/s40409-015-0036-5 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
21 |
container_issue |
1 |
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1766341650626379776 |