Lrp13 is a novel vertebrate lipoprotein receptor that binds vitellogenins in teleost fishes[S]
Transcripts encoding a novel member of the lipoprotein receptor superfamily, termed LDL receptor-related protein (Lrp)13, were sequenced from striped bass (Morone saxatilis) and white perch (Morone americana) ovaries. Receptor proteins were purified from perch ovary membranes by protein-affinity chr...
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ftdoajarticles:oai:doaj.org/article:ee7ae4c28ae0497b87f3610f904510f8 2023-05-15T15:50:38+02:00 Lrp13 is a novel vertebrate lipoprotein receptor that binds vitellogenins in teleost fishes[S] Benjamin J. Reading Naoshi Hiramatsu Justin Schilling Katelyn T. Molloy Norm Glassbrook Hiroko Mizuta Wenshu Luo David A. Baltzegar Valerie N. Williams Takashi Todo Akihiko Hara Craig V. Sullivan 2014-11-01T00:00:00Z https://doi.org/10.1194/jlr.M050286 https://doaj.org/article/ee7ae4c28ae0497b87f3610f904510f8 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S0022227520349816 https://doaj.org/toc/0022-2275 0022-2275 doi:10.1194/jlr.M050286 https://doaj.org/article/ee7ae4c28ae0497b87f3610f904510f8 Journal of Lipid Research, Vol 55, Iss 11, Pp 2287-2295 (2014) affinity purification egg endocytosis oocyte oogenesis ovary Biochemistry QD415-436 article 2014 ftdoajarticles https://doi.org/10.1194/jlr.M050286 2022-12-31T06:06:37Z Transcripts encoding a novel member of the lipoprotein receptor superfamily, termed LDL receptor-related protein (Lrp)13, were sequenced from striped bass (Morone saxatilis) and white perch (Morone americana) ovaries. Receptor proteins were purified from perch ovary membranes by protein-affinity chromatography employing an immobilized mixture of vitellogenins Aa and Ab. RT-PCR revealed lrp13 to be predominantly expressed in striped bass ovary, and in situ hybridization detected lrp13 transcripts in the ooplasm of early secondary growth oocytes. Quantitative RT-PCR confirmed peak lrp13 expression in the ovary during early secondary growth. Quantitative mass spectrometry revealed peak Lrp13 protein levels in striped bass ovary during late-vitellogenesis, and immunohistochemistry localized Lrp13 to the oolemma and zona radiata of vitellogenic oocytes. Previously unreported orthologs of lrp13 were identified in genome sequences of fishes, chicken (Gallus gallus), mouse (Mus musculus), and dog (Canis lupus familiaris). Zebrafish (Danio rerio) and Nile tilapia (Oreochromis niloticus) lrp13 loci are discrete and share genomic synteny. The Lrp13 appears to function as a vitellogenin receptor and may be an important mediator of yolk formation in fishes and other oviparous vertebrates. The presence of lrp13 orthologs in mammals suggests that this lipoprotein receptor is widely distributed among vertebrates, where it may generally play a role in lipoprotein metabolism. Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles Journal of Lipid Research 55 11 2287 2295 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
affinity purification egg endocytosis oocyte oogenesis ovary Biochemistry QD415-436 |
spellingShingle |
affinity purification egg endocytosis oocyte oogenesis ovary Biochemistry QD415-436 Benjamin J. Reading Naoshi Hiramatsu Justin Schilling Katelyn T. Molloy Norm Glassbrook Hiroko Mizuta Wenshu Luo David A. Baltzegar Valerie N. Williams Takashi Todo Akihiko Hara Craig V. Sullivan Lrp13 is a novel vertebrate lipoprotein receptor that binds vitellogenins in teleost fishes[S] |
topic_facet |
affinity purification egg endocytosis oocyte oogenesis ovary Biochemistry QD415-436 |
description |
Transcripts encoding a novel member of the lipoprotein receptor superfamily, termed LDL receptor-related protein (Lrp)13, were sequenced from striped bass (Morone saxatilis) and white perch (Morone americana) ovaries. Receptor proteins were purified from perch ovary membranes by protein-affinity chromatography employing an immobilized mixture of vitellogenins Aa and Ab. RT-PCR revealed lrp13 to be predominantly expressed in striped bass ovary, and in situ hybridization detected lrp13 transcripts in the ooplasm of early secondary growth oocytes. Quantitative RT-PCR confirmed peak lrp13 expression in the ovary during early secondary growth. Quantitative mass spectrometry revealed peak Lrp13 protein levels in striped bass ovary during late-vitellogenesis, and immunohistochemistry localized Lrp13 to the oolemma and zona radiata of vitellogenic oocytes. Previously unreported orthologs of lrp13 were identified in genome sequences of fishes, chicken (Gallus gallus), mouse (Mus musculus), and dog (Canis lupus familiaris). Zebrafish (Danio rerio) and Nile tilapia (Oreochromis niloticus) lrp13 loci are discrete and share genomic synteny. The Lrp13 appears to function as a vitellogenin receptor and may be an important mediator of yolk formation in fishes and other oviparous vertebrates. The presence of lrp13 orthologs in mammals suggests that this lipoprotein receptor is widely distributed among vertebrates, where it may generally play a role in lipoprotein metabolism. |
format |
Article in Journal/Newspaper |
author |
Benjamin J. Reading Naoshi Hiramatsu Justin Schilling Katelyn T. Molloy Norm Glassbrook Hiroko Mizuta Wenshu Luo David A. Baltzegar Valerie N. Williams Takashi Todo Akihiko Hara Craig V. Sullivan |
author_facet |
Benjamin J. Reading Naoshi Hiramatsu Justin Schilling Katelyn T. Molloy Norm Glassbrook Hiroko Mizuta Wenshu Luo David A. Baltzegar Valerie N. Williams Takashi Todo Akihiko Hara Craig V. Sullivan |
author_sort |
Benjamin J. Reading |
title |
Lrp13 is a novel vertebrate lipoprotein receptor that binds vitellogenins in teleost fishes[S] |
title_short |
Lrp13 is a novel vertebrate lipoprotein receptor that binds vitellogenins in teleost fishes[S] |
title_full |
Lrp13 is a novel vertebrate lipoprotein receptor that binds vitellogenins in teleost fishes[S] |
title_fullStr |
Lrp13 is a novel vertebrate lipoprotein receptor that binds vitellogenins in teleost fishes[S] |
title_full_unstemmed |
Lrp13 is a novel vertebrate lipoprotein receptor that binds vitellogenins in teleost fishes[S] |
title_sort |
lrp13 is a novel vertebrate lipoprotein receptor that binds vitellogenins in teleost fishes[s] |
publisher |
Elsevier |
publishDate |
2014 |
url |
https://doi.org/10.1194/jlr.M050286 https://doaj.org/article/ee7ae4c28ae0497b87f3610f904510f8 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Journal of Lipid Research, Vol 55, Iss 11, Pp 2287-2295 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S0022227520349816 https://doaj.org/toc/0022-2275 0022-2275 doi:10.1194/jlr.M050286 https://doaj.org/article/ee7ae4c28ae0497b87f3610f904510f8 |
op_doi |
https://doi.org/10.1194/jlr.M050286 |
container_title |
Journal of Lipid Research |
container_volume |
55 |
container_issue |
11 |
container_start_page |
2287 |
op_container_end_page |
2295 |
_version_ |
1766385634969124864 |