Transient Receptor Potential (TRP) Channels in the Pacific Oyster ( Crassostrea gigas ): Genome-Wide Identification and Expression Profiling after Heat Stress between C. gigas and C. angulata
Transmembrane proteins are involved in an array of stress responses, particularly in thermo-sensation and thermo-regulation. In this study, we performed a genome-wide identification and characterization of the Transient Receptor Potential (TRP) genes in the Pacific oyster ( Crassostrea gigas ) and i...
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ftdoajarticles:oai:doaj.org/article:ee5006f5d3b343829b833466bc0126cc 2023-05-15T15:57:36+02:00 Transient Receptor Potential (TRP) Channels in the Pacific Oyster ( Crassostrea gigas ): Genome-Wide Identification and Expression Profiling after Heat Stress between C. gigas and C. angulata Huiru Fu Zexin Jiao Yongjing Li Jing Tian Liting Ren Fuqiang Zhang Qi Li Shikai Liu 2021-03-01T00:00:00Z https://doi.org/10.3390/ijms22063222 https://doaj.org/article/ee5006f5d3b343829b833466bc0126cc EN eng MDPI AG https://www.mdpi.com/1422-0067/22/6/3222 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms22063222 1422-0067 1661-6596 https://doaj.org/article/ee5006f5d3b343829b833466bc0126cc International Journal of Molecular Sciences, Vol 22, Iss 3222, p 3222 (2021) Crassostrea gigas TRPs phylogeny gene expression thermal stress Biology (General) QH301-705.5 Chemistry QD1-999 article 2021 ftdoajarticles https://doi.org/10.3390/ijms22063222 2022-12-31T06:20:49Z Transmembrane proteins are involved in an array of stress responses, particularly in thermo-sensation and thermo-regulation. In this study, we performed a genome-wide identification and characterization of the Transient Receptor Potential (TRP) genes in the Pacific oyster ( Crassostrea gigas ) and investigated their expression profiles after heat stress to identify critical TRPs potentially associated with thermal regulation. A total of 66 TRP genes were identified in the C. gigas , which showed significant gene expansion and tandem duplication. Meta-analysis of the available RNA-Seq data generated from samples after acute heat stress revealed a set of heat-inducible TRPs. Further examination of their expression profiles under chronic heat stress, and comparison between C. gigas and C. angulata , two oyster species with different tolerance levels to heat stress, led to the identification of TRPC3.6, TRPC3.7, and TRPV4.7 as important TRPs involved in thermal regulation in oysters. This work provided valuable information for future studies on the molecular mechanism of TRP mediated thermal tolerance, and identification of diagnostic biomarker for thermal stress in the oysters. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific International Journal of Molecular Sciences 22 6 3222 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Crassostrea gigas TRPs phylogeny gene expression thermal stress Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
Crassostrea gigas TRPs phylogeny gene expression thermal stress Biology (General) QH301-705.5 Chemistry QD1-999 Huiru Fu Zexin Jiao Yongjing Li Jing Tian Liting Ren Fuqiang Zhang Qi Li Shikai Liu Transient Receptor Potential (TRP) Channels in the Pacific Oyster ( Crassostrea gigas ): Genome-Wide Identification and Expression Profiling after Heat Stress between C. gigas and C. angulata |
topic_facet |
Crassostrea gigas TRPs phylogeny gene expression thermal stress Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
Transmembrane proteins are involved in an array of stress responses, particularly in thermo-sensation and thermo-regulation. In this study, we performed a genome-wide identification and characterization of the Transient Receptor Potential (TRP) genes in the Pacific oyster ( Crassostrea gigas ) and investigated their expression profiles after heat stress to identify critical TRPs potentially associated with thermal regulation. A total of 66 TRP genes were identified in the C. gigas , which showed significant gene expansion and tandem duplication. Meta-analysis of the available RNA-Seq data generated from samples after acute heat stress revealed a set of heat-inducible TRPs. Further examination of their expression profiles under chronic heat stress, and comparison between C. gigas and C. angulata , two oyster species with different tolerance levels to heat stress, led to the identification of TRPC3.6, TRPC3.7, and TRPV4.7 as important TRPs involved in thermal regulation in oysters. This work provided valuable information for future studies on the molecular mechanism of TRP mediated thermal tolerance, and identification of diagnostic biomarker for thermal stress in the oysters. |
format |
Article in Journal/Newspaper |
author |
Huiru Fu Zexin Jiao Yongjing Li Jing Tian Liting Ren Fuqiang Zhang Qi Li Shikai Liu |
author_facet |
Huiru Fu Zexin Jiao Yongjing Li Jing Tian Liting Ren Fuqiang Zhang Qi Li Shikai Liu |
author_sort |
Huiru Fu |
title |
Transient Receptor Potential (TRP) Channels in the Pacific Oyster ( Crassostrea gigas ): Genome-Wide Identification and Expression Profiling after Heat Stress between C. gigas and C. angulata |
title_short |
Transient Receptor Potential (TRP) Channels in the Pacific Oyster ( Crassostrea gigas ): Genome-Wide Identification and Expression Profiling after Heat Stress between C. gigas and C. angulata |
title_full |
Transient Receptor Potential (TRP) Channels in the Pacific Oyster ( Crassostrea gigas ): Genome-Wide Identification and Expression Profiling after Heat Stress between C. gigas and C. angulata |
title_fullStr |
Transient Receptor Potential (TRP) Channels in the Pacific Oyster ( Crassostrea gigas ): Genome-Wide Identification and Expression Profiling after Heat Stress between C. gigas and C. angulata |
title_full_unstemmed |
Transient Receptor Potential (TRP) Channels in the Pacific Oyster ( Crassostrea gigas ): Genome-Wide Identification and Expression Profiling after Heat Stress between C. gigas and C. angulata |
title_sort |
transient receptor potential (trp) channels in the pacific oyster ( crassostrea gigas ): genome-wide identification and expression profiling after heat stress between c. gigas and c. angulata |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/ijms22063222 https://doaj.org/article/ee5006f5d3b343829b833466bc0126cc |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
International Journal of Molecular Sciences, Vol 22, Iss 3222, p 3222 (2021) |
op_relation |
https://www.mdpi.com/1422-0067/22/6/3222 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms22063222 1422-0067 1661-6596 https://doaj.org/article/ee5006f5d3b343829b833466bc0126cc |
op_doi |
https://doi.org/10.3390/ijms22063222 |
container_title |
International Journal of Molecular Sciences |
container_volume |
22 |
container_issue |
6 |
container_start_page |
3222 |
_version_ |
1766393280794198016 |