Smqnr VARIANTS IN CLINICAL ISOLATES OF Stenotrophomonas maltophilia IN BRAZIL

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: Jorge Isaac Gracia-Paez, Juliana Rosa Ferraz, Ivan Avelino Franca E Silva, Flavia Rossi, Anna Sara Levin, Silvia Figueiredo Costa
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 2013
• Subjects: Stenotrophomonas maltophilia; Levofloxacin resistance; qnr genes; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic
• Online Access: https://doi.org/10.1590/S0036-46652013000600008; https://doaj.org/article/ee479223c6c84cf6b52d250426c8bc42

SUMMARY Stenotrophomonas maltophilia contains a novel chromosomally-encoded qnr gene named Smqnr that contributes to low intrinsic resistance to quinolone. We described Smqnr in 13 clinical isolates of S. maltophilia from two Brazilian hospitals, over a 2-year period. The strains were identified by API 20 NE (bioMérieux, France). Susceptibility by microdilution method to trimetroprim/sulfamethoxazole, ciprofloxacin, levofloxacin, minocycline, ceftazidime, chloramphenicol and ticarcillin/clavulanate was performed according to CLSI. PCR detection of Smqnr gene was carried out. The sequence of Smqnr was compared with those deposited in GenBank. Pulsed-field gel electrophoresis (PFGE) of all strains was performed. Thirteen Smqnr positives isolates were sequenced and three novel variants of Smqnr were identified. All 13 Smqnr isolates had distinguishable patterns by PFGE. This is the first report of Smqnr in S. maltophilia isolated in Brazil.