First molecular subtyping and phylogeny of Blastocystis sp. isolated from domestic and synanthropic animals (dogs, cats and brown rats) in southern Iran
Abstract Background Blastocystis sp. is a common intestinal protist that infects humans and many animals globally. Thus far, 22 subtypes (STs) have been identified in mammalian and avian hosts. Since various STs are common to humans and animals, it was suggested that some human infections might aris...
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ftdoajarticles:oai:doaj.org/article:edaac9540e1944eda81c9995a3458347 2023-05-15T15:51:18+02:00 First molecular subtyping and phylogeny of Blastocystis sp. isolated from domestic and synanthropic animals (dogs, cats and brown rats) in southern Iran Iraj Mohammadpour Farzaneh Bozorg-Ghalati Alessia Libera Gazzonis Maria Teresa Manfredi Mohammad Hossein Motazedian Niloofar Mohammadpour 2020-07-01T00:00:00Z https://doi.org/10.1186/s13071-020-04225-9 https://doaj.org/article/edaac9540e1944eda81c9995a3458347 EN eng BMC http://link.springer.com/article/10.1186/s13071-020-04225-9 https://doaj.org/toc/1756-3305 doi:10.1186/s13071-020-04225-9 1756-3305 https://doaj.org/article/edaac9540e1944eda81c9995a3458347 Parasites & Vectors, Vol 13, Iss 1, Pp 1-11 (2020) Blastocystis Subtyping Phylogenetic analysis Canis lupus familiaris Felis catus domesticus Rattus norvegicus Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s13071-020-04225-9 2022-12-31T11:34:32Z Abstract Background Blastocystis sp. is a common intestinal protist that infects humans and many animals globally. Thus far, 22 subtypes (STs) have been identified in mammalian and avian hosts. Since various STs are common to humans and animals, it was suggested that some human infections might arise from zoonotic transmission. Therefore, the aim of this study was to assess the presence of Blastocystis sp. in domestic (dogs and cats) and synanthropic animals (rats) of Fars Province, Iran, and to genetically characterize the samples. Methods A total of 400 fresh faecal samples from 154 dogs, 119 cats, and 127 rats were inspected by direct microscopy, Wheatley’s trichrome staining, in vitro culture, and 18S rRNA gene nested-PCR. Finally, sequencing and phylogenetic analyses were performed. Results Out of 400 samples, 47 (11.8%) and 61 (15.3%) samples were detected as positive by direct wet mount and culture, respectively. Molecular analysis detected a larger number of positive samples (n = 70, 17.5%): nested-PCR showed that 29 (18.8%) dogs, 21 (17.7%) cats, and 20 (15.8%) rats were infected by Blastocystis sp. Sequence analysis of positive samples indicated the presence of zoonotic STs in all investigated host species. Specifically, ST2 (allele 9), ST3 (allele 34), ST4 (allele 94), ST7 (allele 99), ST8 (allele 21), and ST10 (allele 152) were detected in dogs; ST1 (allele 2), ST3 (allele 34), ST4 (allele 94), ST10 (allele 152), and ST14 (allele 159) were detected in cats; and ST1 (allele 2), ST3 (allele 34), and ST4 (allele 92) were detected in rats. Conclusions Our data suggest that domestic dogs and cats can serve as possible reservoirs for in-contact humans, especially those who handle shelter-resident and client-owned animals. Moreover, rats as synanthropic animals can function as a potential source of human infections. Conversely, humans can act as a source of infections to animals. These results should be reinforced in future molecular epidemiological studies. Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles Parasites & Vectors 13 1 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Blastocystis Subtyping Phylogenetic analysis Canis lupus familiaris Felis catus domesticus Rattus norvegicus Infectious and parasitic diseases RC109-216 |
spellingShingle |
Blastocystis Subtyping Phylogenetic analysis Canis lupus familiaris Felis catus domesticus Rattus norvegicus Infectious and parasitic diseases RC109-216 Iraj Mohammadpour Farzaneh Bozorg-Ghalati Alessia Libera Gazzonis Maria Teresa Manfredi Mohammad Hossein Motazedian Niloofar Mohammadpour First molecular subtyping and phylogeny of Blastocystis sp. isolated from domestic and synanthropic animals (dogs, cats and brown rats) in southern Iran |
topic_facet |
Blastocystis Subtyping Phylogenetic analysis Canis lupus familiaris Felis catus domesticus Rattus norvegicus Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Blastocystis sp. is a common intestinal protist that infects humans and many animals globally. Thus far, 22 subtypes (STs) have been identified in mammalian and avian hosts. Since various STs are common to humans and animals, it was suggested that some human infections might arise from zoonotic transmission. Therefore, the aim of this study was to assess the presence of Blastocystis sp. in domestic (dogs and cats) and synanthropic animals (rats) of Fars Province, Iran, and to genetically characterize the samples. Methods A total of 400 fresh faecal samples from 154 dogs, 119 cats, and 127 rats were inspected by direct microscopy, Wheatley’s trichrome staining, in vitro culture, and 18S rRNA gene nested-PCR. Finally, sequencing and phylogenetic analyses were performed. Results Out of 400 samples, 47 (11.8%) and 61 (15.3%) samples were detected as positive by direct wet mount and culture, respectively. Molecular analysis detected a larger number of positive samples (n = 70, 17.5%): nested-PCR showed that 29 (18.8%) dogs, 21 (17.7%) cats, and 20 (15.8%) rats were infected by Blastocystis sp. Sequence analysis of positive samples indicated the presence of zoonotic STs in all investigated host species. Specifically, ST2 (allele 9), ST3 (allele 34), ST4 (allele 94), ST7 (allele 99), ST8 (allele 21), and ST10 (allele 152) were detected in dogs; ST1 (allele 2), ST3 (allele 34), ST4 (allele 94), ST10 (allele 152), and ST14 (allele 159) were detected in cats; and ST1 (allele 2), ST3 (allele 34), and ST4 (allele 92) were detected in rats. Conclusions Our data suggest that domestic dogs and cats can serve as possible reservoirs for in-contact humans, especially those who handle shelter-resident and client-owned animals. Moreover, rats as synanthropic animals can function as a potential source of human infections. Conversely, humans can act as a source of infections to animals. These results should be reinforced in future molecular epidemiological studies. |
format |
Article in Journal/Newspaper |
author |
Iraj Mohammadpour Farzaneh Bozorg-Ghalati Alessia Libera Gazzonis Maria Teresa Manfredi Mohammad Hossein Motazedian Niloofar Mohammadpour |
author_facet |
Iraj Mohammadpour Farzaneh Bozorg-Ghalati Alessia Libera Gazzonis Maria Teresa Manfredi Mohammad Hossein Motazedian Niloofar Mohammadpour |
author_sort |
Iraj Mohammadpour |
title |
First molecular subtyping and phylogeny of Blastocystis sp. isolated from domestic and synanthropic animals (dogs, cats and brown rats) in southern Iran |
title_short |
First molecular subtyping and phylogeny of Blastocystis sp. isolated from domestic and synanthropic animals (dogs, cats and brown rats) in southern Iran |
title_full |
First molecular subtyping and phylogeny of Blastocystis sp. isolated from domestic and synanthropic animals (dogs, cats and brown rats) in southern Iran |
title_fullStr |
First molecular subtyping and phylogeny of Blastocystis sp. isolated from domestic and synanthropic animals (dogs, cats and brown rats) in southern Iran |
title_full_unstemmed |
First molecular subtyping and phylogeny of Blastocystis sp. isolated from domestic and synanthropic animals (dogs, cats and brown rats) in southern Iran |
title_sort |
first molecular subtyping and phylogeny of blastocystis sp. isolated from domestic and synanthropic animals (dogs, cats and brown rats) in southern iran |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s13071-020-04225-9 https://doaj.org/article/edaac9540e1944eda81c9995a3458347 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Parasites & Vectors, Vol 13, Iss 1, Pp 1-11 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s13071-020-04225-9 https://doaj.org/toc/1756-3305 doi:10.1186/s13071-020-04225-9 1756-3305 https://doaj.org/article/edaac9540e1944eda81c9995a3458347 |
op_doi |
https://doi.org/10.1186/s13071-020-04225-9 |
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Parasites & Vectors |
container_volume |
13 |
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1 |
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1766386475502403584 |