Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment.
Distinct lineages of Gammaproteobacteria clade Woeseiales are globally distributed in marine sediments, based on metagenomic and 16S rRNA gene analysis. Yet little is known about why they are dominant or their ecological role in Arctic fjord sediments, where glacial retreat is rapidly imposing chang...
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ftdoajarticles:oai:doaj.org/article:eda30990ee084971bc591184d733781f 2023-05-15T14:54:45+02:00 Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment. Joy Buongiorno Katie Sipes Kenneth Wasmund Alexander Loy Karen G Lloyd 2020-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0234839 https://doaj.org/article/eda30990ee084971bc591184d733781f EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pone.0234839 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0234839 https://doaj.org/article/eda30990ee084971bc591184d733781f PLoS ONE, Vol 15, Iss 8, p e0234839 (2020) Medicine R Science Q article 2020 ftdoajarticles https://doi.org/10.1371/journal.pone.0234839 2022-12-31T10:01:33Z Distinct lineages of Gammaproteobacteria clade Woeseiales are globally distributed in marine sediments, based on metagenomic and 16S rRNA gene analysis. Yet little is known about why they are dominant or their ecological role in Arctic fjord sediments, where glacial retreat is rapidly imposing change. This study combined 16S rRNA gene analysis, metagenome-assembled genomes (MAGs), and genome-resolved metatranscriptomics uncovered the in situ abundance and transcriptional activity of Woeseiales with burial in four shallow sediment sites of Kongsfjorden and Van Keulenfjorden of Svalbard (79°N). We present five novel Woeseiales MAGs and show transcriptional evidence for metabolic plasticity during burial, including sulfur oxidation with reverse dissimilatory sulfite reductase (dsrAB) down to 4 cm depth and nitrite reduction down to 6 cm depth. A single stress protein, spore protein SP21 (hspA), had a tenfold higher mRNA abundance than any other transcript, and was a hundredfold higher on average than other transcripts. At three out of the four sites, SP21 transcript abundance increased with depth, while total mRNA abundance and richness decreased, indicating a shift in investment from metabolism and other cellular processes to build-up of spore protein SP21. The SP21 gene in MAGs was often flanked by genes involved in membrane-associated stress response. The ability of Woeseiales to shift from sulfur oxidation to nitrite reduction with burial into marine sediments with decreasing access to overlying oxic bottom waters, as well as enter into a dormant state dominated by SP21, may account for its ubiquity and high abundance in marine sediments worldwide, including those of the rapidly shifting Arctic. Article in Journal/Newspaper Arctic Kongsfjord* Kongsfjorden Svalbard Van Keulenfjorden Directory of Open Access Journals: DOAJ Articles Arctic Svalbard Van Keulenfjorden ENVELOPE(15.489,15.489,77.559,77.559) PLOS ONE 15 8 e0234839 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Joy Buongiorno Katie Sipes Kenneth Wasmund Alexander Loy Karen G Lloyd Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment. |
topic_facet |
Medicine R Science Q |
description |
Distinct lineages of Gammaproteobacteria clade Woeseiales are globally distributed in marine sediments, based on metagenomic and 16S rRNA gene analysis. Yet little is known about why they are dominant or their ecological role in Arctic fjord sediments, where glacial retreat is rapidly imposing change. This study combined 16S rRNA gene analysis, metagenome-assembled genomes (MAGs), and genome-resolved metatranscriptomics uncovered the in situ abundance and transcriptional activity of Woeseiales with burial in four shallow sediment sites of Kongsfjorden and Van Keulenfjorden of Svalbard (79°N). We present five novel Woeseiales MAGs and show transcriptional evidence for metabolic plasticity during burial, including sulfur oxidation with reverse dissimilatory sulfite reductase (dsrAB) down to 4 cm depth and nitrite reduction down to 6 cm depth. A single stress protein, spore protein SP21 (hspA), had a tenfold higher mRNA abundance than any other transcript, and was a hundredfold higher on average than other transcripts. At three out of the four sites, SP21 transcript abundance increased with depth, while total mRNA abundance and richness decreased, indicating a shift in investment from metabolism and other cellular processes to build-up of spore protein SP21. The SP21 gene in MAGs was often flanked by genes involved in membrane-associated stress response. The ability of Woeseiales to shift from sulfur oxidation to nitrite reduction with burial into marine sediments with decreasing access to overlying oxic bottom waters, as well as enter into a dormant state dominated by SP21, may account for its ubiquity and high abundance in marine sediments worldwide, including those of the rapidly shifting Arctic. |
format |
Article in Journal/Newspaper |
author |
Joy Buongiorno Katie Sipes Kenneth Wasmund Alexander Loy Karen G Lloyd |
author_facet |
Joy Buongiorno Katie Sipes Kenneth Wasmund Alexander Loy Karen G Lloyd |
author_sort |
Joy Buongiorno |
title |
Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment. |
title_short |
Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment. |
title_full |
Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment. |
title_fullStr |
Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment. |
title_full_unstemmed |
Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment. |
title_sort |
woeseiales transcriptional response to shallow burial in arctic fjord surface sediment. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pone.0234839 https://doaj.org/article/eda30990ee084971bc591184d733781f |
long_lat |
ENVELOPE(15.489,15.489,77.559,77.559) |
geographic |
Arctic Svalbard Van Keulenfjorden |
geographic_facet |
Arctic Svalbard Van Keulenfjorden |
genre |
Arctic Kongsfjord* Kongsfjorden Svalbard Van Keulenfjorden |
genre_facet |
Arctic Kongsfjord* Kongsfjorden Svalbard Van Keulenfjorden |
op_source |
PLoS ONE, Vol 15, Iss 8, p e0234839 (2020) |
op_relation |
https://doi.org/10.1371/journal.pone.0234839 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0234839 https://doaj.org/article/eda30990ee084971bc591184d733781f |
op_doi |
https://doi.org/10.1371/journal.pone.0234839 |
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PLOS ONE |
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15 |
container_issue |
8 |
container_start_page |
e0234839 |
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