A novel synthetic DNA vaccine elicits protective immune responses against Powassan virus.
Powassan virus (POWV) infection is a tick-borne emerging infectious disease in the United States and North America. Like Zika virus, POWV is a member of the family Flaviviridae. POWV causes severe neurological sequalae, meningitis, encephalitis, and can cause death. Although the risk of human POWV i...
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ftdoajarticles:oai:doaj.org/article:eda119fe0a6d41708225736a83787c74 2023-05-15T15:05:07+02:00 A novel synthetic DNA vaccine elicits protective immune responses against Powassan virus. Hyeree Choi Sagar B Kudchodkar Michelle Ho Emma L Reuschel Erin Reynolds Ziyang Xu Devivasha Bordoloi Kenneth E Ugen Pablo Tebas Joseph Kim Mohamed Abdel-Mohsen Saravanan Thangamani David B Weiner Kar Muthumani 2020-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008788 https://doaj.org/article/eda119fe0a6d41708225736a83787c74 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008788 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008788 https://doaj.org/article/eda119fe0a6d41708225736a83787c74 PLoS Neglected Tropical Diseases, Vol 14, Iss 10, p e0008788 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008788 2023-01-15T01:29:52Z Powassan virus (POWV) infection is a tick-borne emerging infectious disease in the United States and North America. Like Zika virus, POWV is a member of the family Flaviviridae. POWV causes severe neurological sequalae, meningitis, encephalitis, and can cause death. Although the risk of human POWV infection is low, its incidence in the U.S. in the past 16 years has increased over 300%, urging immediate attention. Despite the disease severity and its growing potential for threatening larger populations, currently there are no licensed vaccines which provide protection against POWV. We developed a novel synthetic DNA vaccine termed POWV-SEV by focusing on the conserved portions of POWV pre-membrane and envelope (prMEnv) genes. A single immunization of POWV-SEV elicited broad T and B cell immunity in mice with minimal cross-reactivity against other flaviviruses. Antibody epitope mapping demonstrated a similarity between POWV-SEV-induced immune responses and those elicited naturally in POWV-infected patients. Finally, POWV-SEV induced immunity provided protection against POWV disease in lethal challenge experiments. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 10 e0008788 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Hyeree Choi Sagar B Kudchodkar Michelle Ho Emma L Reuschel Erin Reynolds Ziyang Xu Devivasha Bordoloi Kenneth E Ugen Pablo Tebas Joseph Kim Mohamed Abdel-Mohsen Saravanan Thangamani David B Weiner Kar Muthumani A novel synthetic DNA vaccine elicits protective immune responses against Powassan virus. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Powassan virus (POWV) infection is a tick-borne emerging infectious disease in the United States and North America. Like Zika virus, POWV is a member of the family Flaviviridae. POWV causes severe neurological sequalae, meningitis, encephalitis, and can cause death. Although the risk of human POWV infection is low, its incidence in the U.S. in the past 16 years has increased over 300%, urging immediate attention. Despite the disease severity and its growing potential for threatening larger populations, currently there are no licensed vaccines which provide protection against POWV. We developed a novel synthetic DNA vaccine termed POWV-SEV by focusing on the conserved portions of POWV pre-membrane and envelope (prMEnv) genes. A single immunization of POWV-SEV elicited broad T and B cell immunity in mice with minimal cross-reactivity against other flaviviruses. Antibody epitope mapping demonstrated a similarity between POWV-SEV-induced immune responses and those elicited naturally in POWV-infected patients. Finally, POWV-SEV induced immunity provided protection against POWV disease in lethal challenge experiments. |
format |
Article in Journal/Newspaper |
author |
Hyeree Choi Sagar B Kudchodkar Michelle Ho Emma L Reuschel Erin Reynolds Ziyang Xu Devivasha Bordoloi Kenneth E Ugen Pablo Tebas Joseph Kim Mohamed Abdel-Mohsen Saravanan Thangamani David B Weiner Kar Muthumani |
author_facet |
Hyeree Choi Sagar B Kudchodkar Michelle Ho Emma L Reuschel Erin Reynolds Ziyang Xu Devivasha Bordoloi Kenneth E Ugen Pablo Tebas Joseph Kim Mohamed Abdel-Mohsen Saravanan Thangamani David B Weiner Kar Muthumani |
author_sort |
Hyeree Choi |
title |
A novel synthetic DNA vaccine elicits protective immune responses against Powassan virus. |
title_short |
A novel synthetic DNA vaccine elicits protective immune responses against Powassan virus. |
title_full |
A novel synthetic DNA vaccine elicits protective immune responses against Powassan virus. |
title_fullStr |
A novel synthetic DNA vaccine elicits protective immune responses against Powassan virus. |
title_full_unstemmed |
A novel synthetic DNA vaccine elicits protective immune responses against Powassan virus. |
title_sort |
novel synthetic dna vaccine elicits protective immune responses against powassan virus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008788 https://doaj.org/article/eda119fe0a6d41708225736a83787c74 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 10, p e0008788 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008788 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008788 https://doaj.org/article/eda119fe0a6d41708225736a83787c74 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008788 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
10 |
container_start_page |
e0008788 |
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1766336862784323584 |